FastQCFastQC Report
Thu 2 Feb 2017
SRR4061993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59308
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT3260.5496728940446483No Hit
CTTTAGGACGTGAAATATGGCGAGG2590.4367033115262696No Hit
GTCCTACAGTGGACATTTCTAAATT2300.38780602954070276No Hit
CTGTAGGACGTGGAATATGGCAAGA2020.34059486072705203No Hit
CTGTAGGACATGGAATATGGCAAGA1400.23605584406825386No Hit
GTCCTTCAGTGTGCATTTCTCATTT1340.22593916503675726No Hit
ATTCCAGGTCCTTCAGTGTGCATTT1270.21413637283334458No Hit
GTATCAACGCAGAGTACTTTTTTTT1110.187158562082687No Hit
CTGAAGGACCTGGAATATGGCGAGA1100.1854724489107709No Hit
ATTTAGAAATGTCCACTGTAGGACG1080.1821002225669387No Hit
TTCCAGGTCCTTCAGTGTGCATTTC930.1568085249881972No Hit
GTCCTACAGTGTGCATTTCTCATTT930.1568085249881972No Hit
TTTCTAAATTTTCCACCTTTTTCAG900.15175018547244892No Hit
GAATATGGCAAGAAAACTGAAAATC870.14669184595670062No Hit
GGAATATGGCGAGAAAACTGAAAAT850.1433196196128684No Hit
TATCAACGCAGAGTACTTTTTTTTT770.1298307142375396No Hit
GTCCTACAGTGGACATTTCTAAATA730.12308626154987523No Hit
CCATATTCCAGGTCCTTCAGTGTGC660.11128346934646254No Hit
GAAATATGGCGAGGAAAACTGAAAA600.10116679031496593No Hit
CCATATTTCACGTCCTAAAGTGTGT600.10116679031496593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA250.00595911119.0033824
GGTATCA307.637685E-418.987331
CCTTTTT456.618391E-414.78040816
TCCACCT456.618391E-414.78040812
GTATCAA1250.014.4303721
GTCCTAC1006.1118044E-1014.2404991
CCTACAG1101.6916601E-1013.8206413
TTCCACC500.00146805813.30236811
CTGTAGG1009.440555E-913.2911321
TCCTACA1153.45608E-1013.2085782
GTCCTAA655.3150503E-513.1450761
TTTTCAG604.0122427E-412.6582219
TGTAGGA1206.8030204E-1012.658222
GTGGAAT701.0553614E-412.21645910
GGACGTG1251.2787496E-912.1621646
CCACCTT550.003004883212.093060513
ACCTTTT550.003004883212.093060515
GTAGGAC1103.5361154E-812.09306053
CTTTTTC550.003004883212.093060517
GGCAAGA1209.304131E-911.86708219
Produced by FastQC (version 0.11.5)