##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061993_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59308
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429587913940782	32.0	32.0	32.0	32.0	32.0
2	31.23715181763	32.0	32.0	32.0	32.0	32.0
3	31.22142038173602	32.0	32.0	32.0	32.0	32.0
4	31.207341336750524	32.0	32.0	32.0	32.0	32.0
5	31.248718553989345	32.0	32.0	32.0	32.0	32.0
6	34.82474539691104	36.0	36.0	36.0	36.0	36.0
7	34.8071086531328	36.0	36.0	36.0	32.0	36.0
8	34.76996357995549	36.0	36.0	36.0	32.0	36.0
9	34.84216294597693	36.0	36.0	36.0	32.0	36.0
10	34.727726444998986	36.0	36.0	36.0	32.0	36.0
11	34.820867336615635	36.0	36.0	36.0	36.0	36.0
12	34.75209078033318	36.0	36.0	36.0	32.0	36.0
13	34.745110271801444	36.0	36.0	36.0	32.0	36.0
14	34.75020233358063	36.0	36.0	36.0	32.0	36.0
15	34.7294968638295	36.0	36.0	36.0	32.0	36.0
16	34.74517771632832	36.0	36.0	36.0	32.0	36.0
17	34.66264247656303	36.0	36.0	36.0	32.0	36.0
18	34.68860861941054	36.0	36.0	36.0	32.0	36.0
19	34.59336008632899	36.0	36.0	36.0	32.0	36.0
20	34.603594793282525	36.0	36.0	36.0	32.0	36.0
21	34.605837323801175	36.0	36.0	36.0	32.0	36.0
22	34.551982869090175	36.0	36.0	36.0	32.0	36.0
23	34.55328117623255	36.0	36.0	36.0	32.0	36.0
24	34.476411276724896	36.0	36.0	36.0	32.0	36.0
25	34.02043569164362	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	4.0
6	8.0
7	1.0
8	9.0
9	6.0
10	1.0
11	0.0
12	0.0
13	4.0
14	6.0
15	10.0
16	31.0
17	34.0
18	50.0
19	58.0
20	60.0
21	89.0
22	110.0
23	130.0
24	191.0
25	283.0
26	347.0
27	482.0
28	683.0
29	1001.0
30	1320.0
31	2050.0
32	3101.0
33	4967.0
34	13259.0
35	31011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.370472786133405	19.07499831388683	13.0707493086936	26.483779591286165
2	14.315100829567681	20.115330140959063	42.103932015916904	23.46563701355635
3	17.697443852431373	24.477304916706007	29.94536993322992	27.879881297632696
4	11.911908535968433	17.29621260665745	37.67326565714478	33.11861320022933
5	12.428320852728868	38.15691830263779	35.54442420562639	13.87033663900695
6	31.230071027280548	37.22099438192769	18.65773623740995	12.89119835338181
7	29.013533124093012	31.434983632007018	21.491006040970607	18.060477202929366
8	25.46620538351194	37.54788625432453	19.65572525525272	17.33018310691081
9	27.088607594936708	15.689451476793248	19.826160337552743	37.3957805907173
10	16.341171108822984	28.621102915112335	33.69680806171193	21.34091791435275
11	33.47286690859988	21.563001097138997	25.94986918727319	19.01426280698793
12	26.316144822347876	23.52941176470588	30.71989197400625	19.434551438939994
13	29.17060491493384	22.886848501215233	25.038819875776397	22.903726708074533
14	20.993384189563223	23.059137244312428	26.60500911361642	29.34246945250793
15	21.576735472328647	33.78284865567351	22.21134533916184	22.429070532835997
16	20.47560378727785	27.731177532868646	29.290644883630655	22.50257379622285
17	20.87594936708861	29.741772151898733	27.851476793248946	21.530801687763713
18	20.751628472105033	26.86725842924162	31.934928617233115	20.44618448142023
19	23.926908281028545	26.397043936019436	27.26429101707498	22.411756765877033
20	24.991985421904264	27.96497207552264	27.497595626571282	19.545446876001822
21	22.836027401883037	26.365875881618468	25.82424999156346	24.97384672493504
22	24.168944686308258	29.39826533022848	26.46890081333738	19.96388917012588
23	21.325774830861636	29.10023451603651	28.0052639570785	21.56872669602335
24	23.26550583788891	26.950462306809747	28.70857798474725	21.075453870554092
25	22.297958495022776	28.621562341825545	27.884258478150837	21.196220685000846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	7.5
3	13.0
4	13.0
5	13.0
6	35.5
7	58.0
8	58.0
9	58.0
10	73.5
11	89.0
12	89.0
13	89.0
14	141.0
15	193.0
16	193.0
17	193.0
18	365.0
19	537.0
20	537.0
21	537.0
22	951.5
23	1366.0
24	1366.0
25	1366.0
26	2143.0
27	2920.0
28	2920.0
29	2920.0
30	3952.5
31	4985.0
32	4985.0
33	4985.0
34	5451.5
35	5918.0
36	5918.0
37	5918.0
38	6508.0
39	7098.0
40	7098.0
41	7098.0
42	7402.0
43	7706.0
44	7706.0
45	7706.0
46	7940.5
47	8175.0
48	8175.0
49	8175.0
50	7486.0
51	6797.0
52	6797.0
53	6797.0
54	5812.5
55	4828.0
56	4828.0
57	4828.0
58	4061.0
59	3294.0
60	3294.0
61	3294.0
62	2753.0
63	2212.0
64	2212.0
65	2212.0
66	1697.0
67	1182.0
68	1182.0
69	1182.0
70	945.5
71	709.0
72	709.0
73	709.0
74	617.0
75	525.0
76	525.0
77	525.0
78	448.0
79	371.0
80	371.0
81	371.0
82	272.5
83	174.0
84	174.0
85	174.0
86	139.0
87	104.0
88	104.0
89	104.0
90	56.5
91	9.0
92	9.0
93	9.0
94	5.5
95	2.0
96	2.0
97	2.0
98	21.5
99	41.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.010116679031496593
5	0.026977810750657583
6	0.059013961017063465
7	0.07756120590814056
8	0.08936399811155324
9	0.09779456397113373
10	0.10959735617454643
11	0.10622512983071423
12	0.10622512983071423
13	0.10116679031496593
14	0.09442233762730154
15	0.09948067714304984
16	0.09610845079921763
17	0.09779456397113373
18	0.08430565859580494
19	0.06744452687664396
20	0.06913064004856005
21	0.07081675322047616
22	0.07756120590814056
23	0.06238618736089566
24	0.06744452687664396
25	0.06407230053281177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	59308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.66641937006812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.37212592854617	30.683887502529174
2	16.74566678457729	15.964119511701625
3	7.435443933498408	10.63262966210292
4	3.80969225327202	7.263775544614555
5	2.3735408560311284	5.656909691778512
6	1.3371064732932436	3.8241046739057127
7	0.9939865581888928	3.3165846091589666
8	0.5978068623983022	2.279625008430566
9	0.4633887513264945	1.9879274296890805
>10	1.7792713123452424	13.556349902205437
>50	0.056597099398655815	1.888446752546031
>100	0.035373187124159884	2.945639711337425
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	326	0.5496728940446483	No Hit
CTTTAGGACGTGAAATATGGCGAGG	259	0.4367033115262696	No Hit
GTCCTACAGTGGACATTTCTAAATT	230	0.38780602954070276	No Hit
CTGTAGGACGTGGAATATGGCAAGA	202	0.34059486072705203	No Hit
CTGTAGGACATGGAATATGGCAAGA	140	0.23605584406825386	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	134	0.22593916503675726	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	127	0.21413637283334458	No Hit
GTATCAACGCAGAGTACTTTTTTTT	111	0.187158562082687	No Hit
CTGAAGGACCTGGAATATGGCGAGA	110	0.1854724489107709	No Hit
ATTTAGAAATGTCCACTGTAGGACG	108	0.1821002225669387	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	93	0.1568085249881972	No Hit
GTCCTACAGTGTGCATTTCTCATTT	93	0.1568085249881972	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	90	0.15175018547244892	No Hit
GAATATGGCAAGAAAACTGAAAATC	87	0.14669184595670062	No Hit
GGAATATGGCGAGAAAACTGAAAAT	85	0.1433196196128684	No Hit
TATCAACGCAGAGTACTTTTTTTTT	77	0.1298307142375396	No Hit
GTCCTACAGTGGACATTTCTAAATA	73	0.12308626154987523	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	66	0.11128346934646254	No Hit
GAAATATGGCGAGGAAAACTGAAAA	60	0.10116679031496593	No Hit
CCATATTTCACGTCCTAAAGTGTGT	60	0.10116679031496593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.001686113171916099	0.0
8	0.0	0.0	0.0	0.001686113171916099	0.0
9	0.0	0.0	0.0	0.001686113171916099	0.0
10	0.0	0.0	0.0	0.001686113171916099	0.0
11	0.0	0.0	0.0	0.001686113171916099	0.0
12	0.0	0.0	0.0	0.001686113171916099	0.0
13	0.0	0.0	0.0	0.001686113171916099	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	25	0.005959111	19.003382	4
GGTATCA	30	7.637685E-4	18.98733	1
CCTTTTT	45	6.618391E-4	14.780408	16
TCCACCT	45	6.618391E-4	14.780408	12
GTATCAA	125	0.0	14.430372	1
GTCCTAC	100	6.1118044E-10	14.240499	1
CCTACAG	110	1.6916601E-10	13.820641	3
TTCCACC	50	0.001468058	13.302368	11
CTGTAGG	100	9.440555E-9	13.291132	1
TCCTACA	115	3.45608E-10	13.208578	2
GTCCTAA	65	5.3150503E-5	13.145076	1
TTTTCAG	60	4.0122427E-4	12.65822	19
TGTAGGA	120	6.8030204E-10	12.65822	2
GTGGAAT	70	1.0553614E-4	12.216459	10
GGACGTG	125	1.2787496E-9	12.162164	6
CCACCTT	55	0.0030048832	12.0930605	13
ACCTTTT	55	0.0030048832	12.0930605	15
GTAGGAC	110	3.5361154E-8	12.0930605	3
CTTTTTC	55	0.0030048832	12.0930605	17
GGCAAGA	120	9.304131E-9	11.867082	19
>>END_MODULE