FastQCFastQC Report
Thu 2 Feb 2017
SRR4061993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59308
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA1030.17366965670735818No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC970.1635529776758616No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA920.1551224118162811No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA650.10959735617454643No Hit
CCCCAGAACCATGCAATGCAAGAATTGTCCATGAAACCACAGCCTTGGAA610.10285290348688203No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT610.10285290348688203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA351.4152647E-744.01
GTGGAAA554.857475E-628.039
AAATTTA655.7729085E-727.07692144
GAAATGT655.7729085E-727.0769216
ATTTAGA655.7729085E-727.0769211
AAATGTC609.550553E-625.6666667
ATGTCCA701.1022075E-625.1428599
TTTAGAA801.2665623E-724.752
AATGTCC651.7759758E-523.6923078
GTATCAA952.5778718E-823.1578941
TTAGAAA803.5205449E-622.03
TAGAAAT803.5205449E-622.04
ATGGGAG602.8284267E-422.05
GGCGAGG1004.4397893E-822.019
AGGTGGA903.948262E-722.037
GCGAGGA1004.4397893E-822.020
CGAGGAA1057.438939E-820.95238121
AAAAGGT956.642713E-720.84210634
GGTGGAA956.642713E-720.84210638
TCTCGCC654.8565376E-420.30769243
Produced by FastQC (version 0.11.5)