##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59308
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.165070479530588	32.0	32.0	32.0	32.0	32.0
2	31.53478451473663	32.0	32.0	32.0	32.0	32.0
3	31.608450799217643	32.0	32.0	32.0	32.0	32.0
4	31.644196398462263	32.0	32.0	32.0	32.0	32.0
5	31.62399676266271	32.0	32.0	32.0	32.0	32.0
6	35.26325284953126	36.0	36.0	36.0	36.0	36.0
7	35.2741114183584	36.0	36.0	36.0	36.0	36.0
8	35.22504552505564	36.0	36.0	36.0	36.0	36.0
9	35.3316584609159	36.0	36.0	36.0	36.0	36.0
10	35.207864031833815	36.0	36.0	36.0	36.0	36.0
11	35.308811627436434	36.0	36.0	36.0	36.0	36.0
12	35.26256154313077	36.0	36.0	36.0	36.0	36.0
13	35.27947325824509	36.0	36.0	36.0	36.0	36.0
14	35.246830107236796	36.0	36.0	36.0	36.0	36.0
15	35.2062622243205	36.0	36.0	36.0	36.0	36.0
16	35.26792338301747	36.0	36.0	36.0	36.0	36.0
17	35.24881972077966	36.0	36.0	36.0	36.0	36.0
18	35.215148040736494	36.0	36.0	36.0	36.0	36.0
19	35.205031361705	36.0	36.0	36.0	36.0	36.0
20	35.21270317663721	36.0	36.0	36.0	36.0	36.0
21	35.18650097794564	36.0	36.0	36.0	36.0	36.0
22	35.16380589465165	36.0	36.0	36.0	36.0	36.0
23	35.14122883927969	36.0	36.0	36.0	36.0	36.0
24	35.13360760774263	36.0	36.0	36.0	36.0	36.0
25	35.06854050043839	36.0	36.0	36.0	36.0	36.0
26	35.05815404329939	36.0	36.0	36.0	36.0	36.0
27	34.99371079786875	36.0	36.0	36.0	36.0	36.0
28	34.981250421528294	36.0	36.0	36.0	36.0	36.0
29	34.90690969177851	36.0	36.0	36.0	36.0	36.0
30	34.86202535914211	36.0	36.0	36.0	32.0	36.0
31	34.87190598232954	36.0	36.0	36.0	32.0	36.0
32	34.7741114183584	36.0	36.0	36.0	32.0	36.0
33	34.79662102920348	36.0	36.0	36.0	32.0	36.0
34	34.8026235920955	36.0	36.0	36.0	32.0	36.0
35	34.75328792068524	36.0	36.0	36.0	32.0	36.0
36	34.699028798812975	36.0	36.0	36.0	32.0	36.0
37	34.638851419707294	36.0	36.0	36.0	32.0	36.0
38	34.63446752546031	36.0	36.0	36.0	32.0	36.0
39	34.47460713563094	36.0	36.0	36.0	32.0	36.0
40	34.575453564443244	36.0	36.0	36.0	32.0	36.0
41	34.55363525999865	36.0	36.0	36.0	32.0	36.0
42	34.541680717609765	36.0	36.0	36.0	32.0	36.0
43	34.579921764348825	36.0	36.0	36.0	32.0	36.0
44	34.469666824037226	36.0	36.0	36.0	32.0	36.0
45	34.44181223443717	36.0	36.0	36.0	32.0	36.0
46	34.35956363391111	36.0	36.0	36.0	32.0	36.0
47	34.35474135023943	36.0	36.0	36.0	32.0	36.0
48	34.34674917380455	36.0	36.0	36.0	32.0	36.0
49	34.25129830714238	36.0	36.0	36.0	32.0	36.0
50	33.87232751062251	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	6.0
23	13.0
24	31.0
25	69.0
26	149.0
27	264.0
28	493.0
29	858.0
30	1301.0
31	1875.0
32	2762.0
33	4623.0
34	10171.0
35	36691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.601639316614104	20.06476312549542	13.556406321150893	26.777191236739583
2	13.702510834190512	20.442473399322125	41.734819486366625	24.120196280120737
3	17.316382275578338	24.517771632831995	30.32980373642679	27.836042355162878
4	11.661158696971741	17.97733863896945	37.912254670533486	32.449247993525326
5	12.522762527820866	38.39954137721724	35.01888446752546	14.058811627436432
6	31.371821676670937	37.21420381736022	18.838942469818573	12.575032036150265
7	28.991029877925406	31.558980238753627	21.612598637620557	17.837391245700413
8	25.17704188305119	38.10447157213192	19.49484049369394	17.22364605112295
9	26.81088554663789	15.62015242463074	19.874215957375057	37.69474607135631
10	16.136103055237065	28.716193430903083	33.87569973696635	21.272003776893506
11	33.634585553382344	21.54684022391583	25.95939839482026	18.859175827881565
12	25.925676131381937	23.88885141970729	30.845754367033116	19.339718081877656
13	29.483374924124906	22.784447292102247	24.967963849733593	22.764213934039255
14	21.0511229513725	22.76590004721117	26.57820192891347	29.604775072502864
15	21.730626559654684	33.55871046064612	22.110002023335806	22.60066095636339
16	20.398597153840967	27.43306130707493	29.515411074391313	22.65293046469279
17	20.865650502461726	29.545761111485803	28.33681796722196	21.251770418830514
18	21.039320159169083	26.738382680245497	31.606191407567273	20.616105753018143
19	23.860187495784718	26.134754164699537	27.716328319956833	22.288730019558916
20	25.185472448910772	27.601672624266545	27.67923383017468	19.53362109664801
21	23.416739731570782	26.256154313077495	25.82450934106697	24.502596614284748
22	24.677952384164026	29.169757874148512	26.148243070074862	20.0040466716126
23	21.65812369326229	29.272610777635393	27.635394887704862	21.43387064139745
24	23.612328859513052	26.900249544749443	28.222162271531666	21.26525932420584
25	22.87718351655763	28.227220611047414	28.001281446010655	20.8943144263843
26	20.12038848047481	29.001146556956904	28.84265191879679	22.035813043771498
27	21.261887097862008	29.464827679233828	27.240844405476494	22.032440817427666
28	21.216719780127136	28.440150403830916	27.376195052860542	22.966934763181413
29	22.616217309929688	27.52288937224948	26.27008616183587	23.59080715598496
30	20.74256424091185	27.458353004653674	28.097389896809872	23.701692857624604
31	23.291967356848993	26.227490389154923	27.39428070412086	23.08626154987523
32	21.71376542793552	28.63357388547919	27.88325352397653	21.769407162608754
33	21.125311930936803	28.66392392257368	29.84251702974304	20.368247116746478
34	22.228029945369933	28.083900991434547	29.34342753085587	20.34464153233965
35	21.100376009577285	29.30514104574502	28.074257676159643	21.52022526851805
36	20.879139407837055	27.834356241990964	29.080393876036958	22.206110474135023
37	21.061239630403993	28.992715991097324	26.453429554191676	23.492614824307008
38	22.115060362851555	29.680650165239093	27.082349767316384	21.121939704592975
39	23.528419916704603	27.49253882341039	27.033908307619676	21.945132952265332
40	24.293518580967156	26.266270992108993	28.80387131584272	20.636339111081135
41	23.117001365774698	26.68319085436795	28.64923196250021	21.550575817357142
42	22.03524154793019	27.972346345164826	28.68223589916533	21.31017620773965
43	20.95199554858617	29.1331546023235	28.344040332507127	21.570809516583203
44	20.42794995447341	28.238289549118136	28.19613529828348	23.13762519812498
45	21.30909826667566	28.78195184460781	27.12281648344237	22.78613340527416
46	21.728940446482767	27.07560531462872	27.965873069400416	23.229581169488096
47	23.177311661158697	26.90193565792136	27.900114655695692	22.020638025224255
48	20.589822277678483	28.548544835261186	27.449161973493407	23.412470913566924
49	22.669252170980524	26.857769159430067	27.925132788129165	22.547845881460248
50	21.798806191616364	29.474252183590195	26.707247158803494	22.01969446598995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	3.5
6	2.0
7	4.5
8	7.0
9	8.5
10	10.0
11	13.0
12	16.0
13	32.5
14	49.0
15	74.5
16	100.0
17	107.0
18	114.0
19	116.0
20	118.0
21	175.0
22	232.0
23	269.5
24	307.0
25	399.0
26	491.0
27	671.5
28	852.0
29	1182.5
30	1513.0
31	2039.0
32	2565.0
33	3188.5
34	3812.0
35	4503.5
36	5195.0
37	5070.0
38	4945.0
39	4561.0
40	4177.0
41	4077.5
42	3978.0
43	3889.0
44	3800.0
45	3828.5
46	3857.0
47	3876.5
48	3896.0
49	3725.5
50	3555.0
51	3348.0
52	3141.0
53	3073.5
54	3006.0
55	2691.0
56	2376.0
57	2085.5
58	1795.0
59	1517.5
60	1240.0
61	1010.0
62	780.0
63	656.5
64	533.0
65	531.5
66	530.0
67	438.0
68	346.0
69	359.5
70	373.0
71	326.5
72	280.0
73	283.5
74	287.0
75	285.0
76	283.0
77	263.5
78	244.0
79	221.0
80	198.0
81	186.0
82	174.0
83	118.5
84	63.0
85	49.5
86	36.0
87	24.0
88	12.0
89	7.5
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	3.5
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025291697578741484
2	0.008430565859580495
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.001686113171916099
29	0.001686113171916099
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.001686113171916099
36	0.0
37	0.0
38	0.0
39	0.001686113171916099
40	0.0
41	0.001686113171916099
42	0.005058339515748297
43	0.001686113171916099
44	0.003372226343832198
45	0.0
46	0.0
47	0.0
48	0.003372226343832198
49	0.005058339515748297
50	0.003372226343832198
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	59308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.972617522088086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.18973803991469	35.59216294597694
2	15.781264920266096	16.719498212720037
3	6.830696756533087	10.855196600795844
4	3.501289110990865	7.418897956430835
5	2.0912244962918165	5.5388817697443855
6	1.2922939809657192	4.107371686787617
7	0.8594073272432122	3.186753894921427
8	0.6238660597765541	2.6438254535644434
9	0.4074227329153007	1.942402374047346
>10	1.3941496641945443	10.907466109125243
>50	0.025463920807206292	0.9138733391785256
>100	0.0031829901009007865	0.17366965670735818
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	103	0.17366965670735818	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	97	0.1635529776758616	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	92	0.1551224118162811	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	65	0.10959735617454643	No Hit
CCCCAGAACCATGCAATGCAAGAATTGTCCATGAAACCACAGCCTTGGAA	61	0.10285290348688203	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	61	0.10285290348688203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.001686113171916099	0.0
8	0.0	0.0	0.0	0.001686113171916099	0.0
9	0.0	0.0	0.0	0.001686113171916099	0.0
10	0.0	0.0	0.0	0.001686113171916099	0.0
11	0.0	0.0	0.0	0.001686113171916099	0.0
12	0.0	0.0	0.0	0.001686113171916099	0.0
13	0.0	0.0	0.0	0.001686113171916099	0.0
14	0.0	0.0	0.0	0.001686113171916099	0.0
15	0.0	0.0	0.0	0.001686113171916099	0.0
16	0.0	0.0	0.0	0.001686113171916099	0.0
17	0.0	0.0	0.0	0.001686113171916099	0.0
18	0.0	0.0	0.0	0.001686113171916099	0.0
19	0.0	0.0	0.0	0.001686113171916099	0.0
20	0.0	0.0	0.0	0.001686113171916099	0.0
21	0.0	0.0	0.0	0.001686113171916099	0.0
22	0.0	0.0	0.0	0.001686113171916099	0.0
23	0.0	0.0	0.0	0.001686113171916099	0.0
24	0.0	0.0	0.0	0.001686113171916099	0.0
25	0.0	0.0	0.0	0.001686113171916099	0.0
26	0.0	0.0	0.0	0.001686113171916099	0.0
27	0.0	0.0	0.0	0.001686113171916099	0.0
28	0.0	0.0	0.0	0.001686113171916099	0.0
29	0.0	0.0	0.0	0.003372226343832198	0.0
30	0.0	0.0	0.0	0.005058339515748297	0.0
31	0.0	0.0	0.0	0.013488905375328791	0.0
32	0.0	0.0	0.0	0.021919471234909286	0.0
33	0.0	0.0	0.0	0.03372226343832198	0.0
34	0.0	0.0	0.0	0.042152829297902476	0.0
35	0.0	0.0	0.0	0.053955621501315165	0.0
36	0.0	0.0	0.0	0.07250286639239226	0.0
37	0.0	0.0	0.0	0.09105011128346935	0.0
38	0.0	0.0	0.0	0.12645848789370742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	1.4152647E-7	44.0	1
GTGGAAA	55	4.857475E-6	28.0	39
AAATTTA	65	5.7729085E-7	27.076921	44
GAAATGT	65	5.7729085E-7	27.076921	6
ATTTAGA	65	5.7729085E-7	27.076921	1
AAATGTC	60	9.550553E-6	25.666666	7
ATGTCCA	70	1.1022075E-6	25.142859	9
TTTAGAA	80	1.2665623E-7	24.75	2
AATGTCC	65	1.7759758E-5	23.692307	8
GTATCAA	95	2.5778718E-8	23.157894	1
TTAGAAA	80	3.5205449E-6	22.0	3
TAGAAAT	80	3.5205449E-6	22.0	4
ATGGGAG	60	2.8284267E-4	22.0	5
GGCGAGG	100	4.4397893E-8	22.0	19
AGGTGGA	90	3.948262E-7	22.0	37
GCGAGGA	100	4.4397893E-8	22.0	20
CGAGGAA	105	7.438939E-8	20.952381	21
AAAAGGT	95	6.642713E-7	20.842106	34
GGTGGAA	95	6.642713E-7	20.842106	38
TCTCGCC	65	4.8565376E-4	20.307692	43
>>END_MODULE