FastQCFastQC Report
Thu 2 Feb 2017
SRR4061992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7247471
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGT101090.1394831383250792No Hit
GCGCAAGACGGACCAGAGCGAAAGC97250.13418473837287517No Hit
ATCAGATACCGTCGTAGTTCCGACC95180.1313285696486402No Hit
GATTAAGAGGGACGGCCGGGGGCAT93700.1292864780003949No Hit
GGGTAGGCACACGCTGAGCCAGTCA93390.12885874258758676No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG92590.12775490926421093No Hit
TCGTAGTTCCGACCATAAACGATGC90400.12473316554146958No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC82330.1135982468919158No Hit
GTATCTGATCGTCTTCGAACCTCCG81390.1123012427369492No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT77400.10679587403661223No Hit
GAACTACGACGGTATCTGATCGTCT76900.10610597820950231No Hit
GTACATGGGGAATAATTGCAATCCC74560.102877265738628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.013.8487951
GTATCAA78050.013.4558441
CGAACGA6150.012.04853516
TCGCGTA11000.012.0066239
GCGTAAC11600.011.54957311
CGCGTAA11450.011.53466810
ACGGTAT13700.010.9581019
CCGACCA21000.010.9495149
CGACCAT20700.010.924520510
CGGTCCA14200.010.63909210
CGGACCA18050.010.580789
CGGACAT12200.010.4354395
CGTCGTA17850.010.37987710
CGGTTCT20250.010.3689812
GGTTCTA20800.010.32254713
CCGTCGT17650.010.2822339
CGCATCG12500.010.26042913
ACGAACG7150.010.23105515
GACCATA21900.010.06580811
TCCGATA7650.010.0601488
Produced by FastQC (version 0.11.5)