##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061992_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7247471
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29082406814736	32.0	32.0	32.0	32.0	32.0
2	30.812631330294387	32.0	32.0	32.0	32.0	32.0
3	30.83078856058893	32.0	32.0	32.0	32.0	32.0
4	30.844663331526267	32.0	32.0	32.0	32.0	32.0
5	30.74765018031807	32.0	32.0	32.0	32.0	32.0
6	34.42061858543484	36.0	36.0	36.0	32.0	36.0
7	34.35192041472122	36.0	36.0	36.0	32.0	36.0
8	34.3145199201211	36.0	36.0	36.0	32.0	36.0
9	34.45416670173637	36.0	36.0	36.0	32.0	36.0
10	34.12839002736265	36.0	36.0	36.0	32.0	36.0
11	34.43060910488638	36.0	36.0	36.0	32.0	36.0
12	34.241485340196604	36.0	36.0	36.0	32.0	36.0
13	34.33871815423615	36.0	36.0	36.0	32.0	36.0
14	34.23814693428922	36.0	36.0	36.0	32.0	36.0
15	34.18002859204266	36.0	36.0	36.0	32.0	36.0
16	34.18840227163379	36.0	36.0	36.0	32.0	36.0
17	34.11211303915531	36.0	36.0	36.0	32.0	36.0
18	34.12832241757159	36.0	36.0	36.0	32.0	36.0
19	34.13078893313268	36.0	36.0	36.0	32.0	36.0
20	34.11580301597619	36.0	36.0	36.0	32.0	36.0
21	34.09693533095889	36.0	36.0	36.0	32.0	36.0
22	34.065847245197666	36.0	36.0	36.0	32.0	36.0
23	34.035303487244036	36.0	36.0	36.0	32.0	36.0
24	34.00925674624983	36.0	36.0	36.0	32.0	36.0
25	33.6591488258456	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	104.0
5	560.0
6	1539.0
7	321.0
8	1299.0
9	1047.0
10	509.0
11	143.0
12	303.0
13	305.0
14	799.0
15	1196.0
16	1968.0
17	2763.0
18	4066.0
19	5650.0
20	8643.0
21	13061.0
22	20245.0
23	29687.0
24	42885.0
25	60645.0
26	83074.0
27	107332.0
28	143097.0
29	189356.0
30	248999.0
31	339220.0
32	482030.0
33	687644.0
34	1512694.0
35	3256282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25192875906221	17.149722259246964	11.930599720782856	25.667749260907975
2	17.379531093069826	19.321027489527008	36.4664486865796	26.83299273082357
3	19.516146057191268	23.062904324081554	27.1978844929275	30.223065125799682
4	13.385619998683543	15.455177841221065	34.228423712420394	36.930778447674996
5	16.078293183425252	35.52176108868867	32.074902142492455	16.325043585393622
6	35.53486563726085	33.738948881644006	16.57075787756121	14.155427603533926
7	31.79589522922337	29.18251013595271	19.911248946070874	19.110345688753043
8	29.05243063373424	31.564327222849187	18.931952200920783	20.45128994249579
9	27.30566486681831	14.861582608508256	18.443605933504063	39.38914659116937
10	17.437108252090358	25.865966154693847	29.29143488435777	27.405490708858025
11	37.51072061163757	21.15680494753032	21.548115044615844	19.78435939621626
12	25.779112445779113	23.035305837238205	27.282202837758398	23.90337887922429
13	29.302364843627927	19.195131686218847	24.803924426576778	26.698579043576448
14	24.511280127376462	19.417989798058134	23.98064156251114	32.090088512054265
15	25.7799661610536	26.652536249204523	21.295020844691507	26.272476745050373
16	26.805335828050204	25.31222181024274	22.710506931846375	25.171935429860675
17	24.894390256908878	25.6528960237576	24.12807894295485	25.324634776378673
18	25.734504619554144	24.458006156642842	25.0760049092907	24.731484314512315
19	26.31989004904342	24.51190341199055	24.227288880503746	24.940917658462286
20	26.62633332131785	23.723821270964425	23.656645065041733	25.993200342675994
21	27.610469457820503	23.69419385293554	23.486494575083793	25.208842114160163
22	26.92082710915805	23.5334807107542	24.028597020821376	25.517095159266372
23	24.99160383351286	23.789239649791476	24.78190681359958	26.43724970309609
24	25.42236505648959	24.363933672296177	24.44987822335225	25.763823047861983
25	25.4918016033304	23.953131421828804	24.281169485458218	26.273897489382573
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	362.0
2	986.5
3	1611.0
4	1611.0
5	1611.0
6	3063.5
7	4516.0
8	4516.0
9	4516.0
10	5405.5
11	6295.0
12	6295.0
13	6295.0
14	8446.5
15	10598.0
16	10598.0
17	10598.0
18	18336.5
19	26075.0
20	26075.0
21	26075.0
22	46475.5
23	66876.0
24	66876.0
25	66876.0
26	105533.5
27	144191.0
28	144191.0
29	144191.0
30	189278.5
31	234366.0
32	234366.0
33	234366.0
34	314339.5
35	394313.0
36	394313.0
37	394313.0
38	475094.0
39	555875.0
40	555875.0
41	555875.0
42	653534.5
43	751194.0
44	751194.0
45	751194.0
46	845920.0
47	940646.0
48	940646.0
49	940646.0
50	978440.5
51	1016235.0
52	1016235.0
53	1016235.0
54	960422.0
55	904609.0
56	904609.0
57	904609.0
58	843548.5
59	782488.0
60	782488.0
61	782488.0
62	692199.5
63	601911.0
64	601911.0
65	601911.0
66	493157.5
67	384404.0
68	384404.0
69	384404.0
70	292902.0
71	201400.0
72	201400.0
73	201400.0
74	153367.5
75	105335.0
76	105335.0
77	105335.0
78	86056.0
79	66777.0
80	66777.0
81	66777.0
82	46730.5
83	26684.0
84	26684.0
85	26684.0
86	19613.0
87	12542.0
88	12542.0
89	12542.0
90	8488.0
91	4434.0
92	4434.0
93	4434.0
94	2695.5
95	957.0
96	957.0
97	957.0
98	1867.0
99	2777.0
100	2777.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.968645752428675E-4
2	2.759583308439592E-4
3	4.967249955191266E-4
4	0.010403629072817264
5	0.03446719552241051
6	0.06418790775430491
7	0.1089345511006529
8	0.11039713025412588
9	0.12022124683217082
10	0.1340743550405376
11	0.14134585705827593
12	0.1340605571239954
13	0.1285689863402006
14	0.11668898019736816
15	0.1332464800480057
16	0.12013845933291764
17	0.12458138845950541
18	0.11225984898732262
19	0.09868269910979982
20	0.09399140748545251
21	0.09263921166431711
22	0.1042570573928478
23	0.08376715132768382
24	0.09321872415908942
25	0.09027976793560126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7247471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.047544179388424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.80495030275408	24.081543974403758
2	15.737223400829167	11.345765116045897
3	6.7151352749304225	7.261924064808722
4	3.3139588374330216	4.7784031040416615
5	1.9376256403199625	3.4923322936274817
6	1.1879001202481771	2.5692529239208195
7	0.7942259340304311	2.0040926111764596
8	0.566964073688007	1.6350129995515574
9	0.4231533313432472	1.3728274565625984
>10	2.2507863415436393	14.652664159544438
>50	0.15248968099376062	3.7883905874668264
>100	0.08738268731484816	6.2899447471234184
>500	0.01289351922706645	3.277544907673206
>1k	0.014006170929387057	10.17530657957221
>5k	0.0012663113439156917	3.1351612292430824
>10k+	3.837307102774823E-5	0.13983324523791346
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGT	10109	0.1394831383250792	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	9725	0.13418473837287517	No Hit
ATCAGATACCGTCGTAGTTCCGACC	9518	0.1313285696486402	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	9370	0.1292864780003949	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	9339	0.12885874258758676	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	9259	0.12775490926421093	No Hit
TCGTAGTTCCGACCATAAACGATGC	9040	0.12473316554146958	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	8233	0.1135982468919158	No Hit
GTATCTGATCGTCTTCGAACCTCCG	8139	0.1123012427369492	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	7740	0.10679587403661223	No Hit
GAACTACGACGGTATCTGATCGTCT	7690	0.10610597820950231	No Hit
GTACATGGGGAATAATTGCAATCCC	7456	0.102877265738628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1038333233758369E-4
2	0.0	0.0	0.0	0.0	1.1038333233758369E-4
3	0.0	0.0	0.0	0.0	1.1038333233758369E-4
4	0.0	0.0	0.0	0.0	1.2418124887978165E-4
5	2.759583308439592E-5	0.0	0.0	0.0	1.3797916542197962E-4
6	2.759583308439592E-5	0.0	0.0	0.0	2.2076666467516737E-4
7	5.519166616879184E-5	0.0	0.0	0.0	2.2076666467516737E-4
8	5.519166616879184E-5	0.0	0.0	0.0	2.3456458121736535E-4
9	5.519166616879184E-5	0.0	0.0	0.0	2.6216041430176124E-4
10	5.519166616879184E-5	0.0	0.0	6.898958271098981E-5	3.0355416392835514E-4
11	5.519166616879184E-5	0.0	0.0	6.898958271098981E-5	3.0355416392835514E-4
12	6.898958271098981E-5	0.0	0.0	8.278749925318776E-5	6.760979105677001E-4
13	6.898958271098981E-5	0.0	0.0	1.1038333233758369E-4	8.002791594474818E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2345	0.0	13.848795	1
GTATCAA	7805	0.0	13.455844	1
CGAACGA	615	0.0	12.048535	16
TCGCGTA	1100	0.0	12.006623	9
GCGTAAC	1160	0.0	11.549573	11
CGCGTAA	1145	0.0	11.534668	10
ACGGTAT	1370	0.0	10.958101	9
CCGACCA	2100	0.0	10.949514	9
CGACCAT	2070	0.0	10.9245205	10
CGGTCCA	1420	0.0	10.639092	10
CGGACCA	1805	0.0	10.58078	9
CGGACAT	1220	0.0	10.435439	5
CGTCGTA	1785	0.0	10.379877	10
CGGTTCT	2025	0.0	10.36898	12
GGTTCTA	2080	0.0	10.322547	13
CCGTCGT	1765	0.0	10.282233	9
CGCATCG	1250	0.0	10.260429	13
ACGAACG	715	0.0	10.231055	15
GACCATA	2190	0.0	10.065808	11
TCCGATA	765	0.0	10.060148	8
>>END_MODULE