FastQCFastQC Report
Thu 2 Feb 2017
SRR4061992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7247471
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT105220.14518167785700695No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA102040.140793940396588No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA98930.13650278835196444No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG98690.1361716383549517No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT95980.13243240297201603No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC93080.12843100717477862No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT92980.12829302800935666No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA79590.10981761775935357No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC75860.10467099488911374No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA75170.10371893864770207No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA73400.10127670741973303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84450.028.637971
GGTATCA31250.024.5060561
TCAACGC132100.018.102274
ATCAACG134500.017.811973
CAACGCA137000.017.4869335
AACGCAG137250.017.4550826
TAGGACG8150.017.0054954
TATCAAC145350.016.6947082
CGCAATA19650.016.34634236
ATACCGT32100.015.9682466
GTATTAC6500.015.9121441
ACCGTCG31700.015.8921478
TACCGTC32750.015.71849257
CCGTCGT32200.015.5770549
CGTCGTA32250.015.48490210
TACGAAT20650.015.34283841
GTCCTAT22800.015.0568441
ATACGAA21750.014.66753140
GTCGTAG33900.014.66631711
CAATACG22050.014.56775238
Produced by FastQC (version 0.11.5)