##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7247471
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330693975871032	32.0	32.0	32.0	32.0	32.0
2	31.47497892713196	32.0	32.0	32.0	32.0	32.0
3	31.58385359527482	32.0	32.0	32.0	32.0	32.0
4	31.659549103404483	32.0	32.0	32.0	32.0	32.0
5	31.631481381574346	32.0	32.0	32.0	32.0	32.0
6	35.288650620333634	36.0	36.0	36.0	36.0	36.0
7	35.287667104842505	36.0	36.0	36.0	36.0	36.0
8	35.24369424865584	36.0	36.0	36.0	36.0	36.0
9	35.32254727200702	36.0	36.0	36.0	36.0	36.0
10	35.206495479595574	36.0	36.0	36.0	36.0	36.0
11	35.32564545618741	36.0	36.0	36.0	36.0	36.0
12	35.243452371178854	36.0	36.0	36.0	36.0	36.0
13	35.283358981360536	36.0	36.0	36.0	36.0	36.0
14	35.242710733164714	36.0	36.0	36.0	36.0	36.0
15	35.21906827912799	36.0	36.0	36.0	36.0	36.0
16	35.23031427100571	36.0	36.0	36.0	36.0	36.0
17	35.20903153665603	36.0	36.0	36.0	36.0	36.0
18	35.208688934388285	36.0	36.0	36.0	36.0	36.0
19	35.200979348520335	36.0	36.0	36.0	36.0	36.0
20	35.20017451604843	36.0	36.0	36.0	36.0	36.0
21	35.19063063515535	36.0	36.0	36.0	36.0	36.0
22	35.179666396733424	36.0	36.0	36.0	36.0	36.0
23	35.144637763986914	36.0	36.0	36.0	36.0	36.0
24	35.12796891494978	36.0	36.0	36.0	36.0	36.0
25	35.11203025165606	36.0	36.0	36.0	36.0	36.0
26	35.07041504546896	36.0	36.0	36.0	36.0	36.0
27	35.058481641389115	36.0	36.0	36.0	36.0	36.0
28	35.0367006642731	36.0	36.0	36.0	36.0	36.0
29	35.01821863102315	36.0	36.0	36.0	36.0	36.0
30	34.998764672531976	36.0	36.0	36.0	36.0	36.0
31	35.00578215490617	36.0	36.0	36.0	36.0	36.0
32	34.982543979824136	36.0	36.0	36.0	36.0	36.0
33	34.959271723888236	36.0	36.0	36.0	36.0	36.0
34	34.950500802279855	36.0	36.0	36.0	36.0	36.0
35	34.936388155261334	36.0	36.0	36.0	36.0	36.0
36	34.91932496176942	36.0	36.0	36.0	32.0	36.0
37	34.908544925533334	36.0	36.0	36.0	32.0	36.0
38	34.88677609058387	36.0	36.0	36.0	32.0	36.0
39	34.88272943762038	36.0	36.0	36.0	32.0	36.0
40	34.86530791223587	36.0	36.0	36.0	32.0	36.0
41	34.843884163179126	36.0	36.0	36.0	32.0	36.0
42	34.81002807738037	36.0	36.0	36.0	32.0	36.0
43	34.81831814159725	36.0	36.0	36.0	32.0	36.0
44	34.76816326688303	36.0	36.0	36.0	32.0	36.0
45	34.76366845759024	36.0	36.0	36.0	32.0	36.0
46	34.75485683212806	36.0	36.0	36.0	32.0	36.0
47	34.73482763849624	36.0	36.0	36.0	32.0	36.0
48	34.710288871800934	36.0	36.0	36.0	32.0	36.0
49	34.704091399606845	36.0	36.0	36.0	32.0	36.0
50	34.2927895813588	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	18.0
20	52.0
21	190.0
22	456.0
23	1294.0
24	3244.0
25	7774.0
26	16124.0
27	30828.0
28	52830.0
29	84780.0
30	127511.0
31	189866.0
32	290119.0
33	500918.0
34	1176072.0
35	4765392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.840353671564664	17.69176303011398	12.346124436518826	26.12175886180253
2	16.746116695020486	19.83305628048024	36.18427089518267	27.236556129316597
3	18.786804303966605	23.494222936772	27.50972962367198	30.209243135589407
4	13.055992374717299	15.856652853568603	34.37044021850939	36.71691455320471
5	15.734373512411917	35.88270113570667	31.898979919889285	16.48394543199213
6	35.61268852986957	33.90836705664702	16.4349187763601	14.0440256371233
7	31.81079303387347	29.216067232280057	19.894939903864397	19.078199829982072
8	29.142786497524447	31.646418454106268	18.803524705376535	20.40727034299275
9	27.444883877424274	14.584039039273147	18.388662748702274	39.5824143346003
10	17.444294997532932	25.88795197878872	29.271618320560126	27.396134703118225
11	37.81049969016778	21.00320235845028	21.501307145623624	19.68499080575831
12	25.76658809673057	22.995759486309087	27.291188885060734	23.94646353189961
13	29.43247375532789	19.040490124072246	24.861927698641363	26.6651084219585
14	24.58188518450091	19.250094274264775	23.959433573449278	32.20858696778504
15	25.841234825223864	26.609751180791203	21.262437614445094	26.286576379539845
16	26.946902367917534	25.21399675443258	22.63132702479212	25.207773852857766
17	25.0315454866946	25.641661760357508	23.997433035606488	25.329359717341397
18	25.88723497611577	24.31377337715558	25.08528505159049	24.713706595138156
19	26.453452032226423	24.352277415429107	24.23985197593091	24.954418576413563
20	26.81336564079306	23.544311936517246	23.68844541024021	25.95387701244948
21	27.793730473355595	23.603067498460497	23.450766053638862	25.152435974545046
22	26.99468545648544	23.460252548785636	24.05098274970676	25.49407924502216
23	25.06240108617214	23.636648375226113	24.82867814561495	26.472272392986795
24	25.572746541071332	24.246119610017548	24.451393140411813	25.729740708499303
25	25.686179276849096	23.732912955591274	24.37215848332862	26.208749284231004
26	25.497310427758237	24.918443559686978	24.601503842393896	24.98274217016089
27	26.1415636002179	23.995519513957227	24.1037786094598	25.75913827636508
28	25.52396422083339	24.045422972768236	24.67597103822558	25.754641768172792
29	25.352200516762206	24.211631316683945	24.763095462476787	25.673072704077054
30	25.218386087865518	24.36558029111522	25.027406489884402	25.38862713113486
31	26.20649859902236	24.097891682841347	23.77223181871555	25.923377899420746
32	25.99042415210972	23.970182410243673	23.671620167232387	26.36777327041422
33	25.243620136238242	23.806825323827645	24.49186617474199	26.457688365192123
34	26.19738011848895	24.01081100920368	24.40765529614855	25.38415357615882
35	26.60541660934765	23.612170296058654	24.66213974047972	25.12027335411398
36	25.207959035585013	24.532853643503564	24.14867772313642	26.110509597775
37	26.428428519587104	24.160930102920094	24.143213331180828	25.267428046311974
38	25.513768871927876	23.85241693274799	24.495413641530956	26.13840055379318
39	26.470065534937063	23.717421563941546	23.885011960098417	25.927500941022974
40	26.419755437178498	24.1120099543799	24.283242287680533	25.184992320761072
41	25.27758966402792	24.812332387889263	24.637552177418954	25.272525770663854
42	26.873082086391957	24.48104650487303	24.057748077757093	24.58812333097792
43	26.192487151907944	23.558264346777406	24.302042793167278	25.94720570814737
44	25.57751540395239	24.289329097746624	23.93163065396874	26.20152484433225
45	25.747567692795297	24.480260818559977	24.070137762325665	25.702033726319062
46	25.520554279145557	24.361643602748213	24.16092121187368	25.95688090623255
47	25.750044084933382	24.012531987448142	24.70723878745598	25.53018514016249
48	26.556878857356065	24.724348409701303	23.501433382750175	25.217339350192457
49	25.667303925593178	24.79208133916824	23.606338185102217	25.934276550136364
50	25.940195252030705	24.823745945242614	23.78030262367588	25.4557561790508
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	63.0
2	61.0
3	173.0
4	285.0
5	326.5
6	368.0
7	337.0
8	306.0
9	426.5
10	547.0
11	887.0
12	1227.0
13	2168.5
14	3110.0
15	4263.0
16	5416.0
17	5467.5
18	5519.0
19	5747.0
20	5975.0
21	6914.0
22	7853.0
23	9940.0
24	12027.0
25	16140.0
26	20253.0
27	26659.0
28	33065.0
29	42113.5
30	51162.0
31	63863.5
32	76565.0
33	92208.0
34	107851.0
35	129179.5
36	150508.0
37	186674.0
38	222840.0
39	253048.5
40	283257.0
41	317901.0
42	352545.0
43	368723.5
44	384902.0
45	427736.0
46	470570.0
47	510481.0
48	550392.0
49	579078.0
50	607764.0
51	588911.0
52	570058.0
53	571449.0
54	572840.0
55	577750.5
56	582661.0
57	565559.5
58	548458.0
59	506250.0
60	464042.0
61	406628.5
62	349215.0
63	298657.0
64	248099.0
65	209500.5
66	170902.0
67	149230.5
68	127559.0
69	115096.5
70	102634.0
71	78555.0
72	54476.0
73	47967.0
74	41458.0
75	30011.5
76	18565.0
77	17011.5
78	15458.0
79	13243.5
80	11029.0
81	8736.5
82	6444.0
83	5625.0
84	4806.0
85	3781.0
86	2756.0
87	1839.0
88	922.0
89	593.5
90	265.0
91	197.5
92	130.0
93	110.0
94	90.0
95	75.5
96	61.0
97	67.0
98	73.0
99	55.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032162943459863445
2	0.00493965412210687
3	2.3456458121736535E-4
4	2.7595833084395925E-5
5	1.3797916542197962E-5
6	1.6557499850637555E-4
7	0.0
8	0.0
9	0.0
10	9.658541579538574E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.622999940255022E-4
17	0.0
18	1.5177708196417757E-4
19	1.3797916542197962E-5
20	3.0355416392835514E-4
21	2.7595833084395925E-5
22	0.0
23	1.3797916542197962E-5
24	9.382583248694614E-4
25	2.897562473861572E-4
26	4.967249955191266E-4
27	5.933104113145123E-4
28	0.0017247395677747453
29	5.105229120613246E-4
30	0.001379791654219796
31	4.415333293503348E-4
32	9.796520744960553E-4
33	2.759583308439592E-4
34	6.071083278567103E-4
35	3.0355416392835514E-4
36	1.379791654219796E-4
37	0.001393589570761994
38	0.0
39	5.381187451457205E-4
40	1.103833323375837E-4
41	0.0011452270730024307
42	0.004663695791262911
43	0.003946204131068617
44	0.004208364545370378
45	0.001848920816654527
46	0.0020420916482452984
47	0.0013383979045932023
48	0.003849618715273231
49	0.003946204131068617
50	0.0016557499850637554
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7247471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.285636488030484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.06616748514922	23.609673010354328
2	16.65652854961249	12.087854802074807
3	7.058991193270353	7.684199652336497
4	3.5653887960558834	5.174896071687217
5	2.0184482605769793	3.6620339926596843
6	1.2454380436027246	2.7114907271119395
7	0.8498527972079369	2.1586214767476157
8	0.5831991639782579	1.6929402291390676
9	0.44438428311657674	1.4512289902345912
>10	2.2564373553394015	14.773541089098313
>50	0.1492533690602753	3.718627071434391
>100	0.08019428845509753	5.843959747989723
>500	0.011710254924080477	2.983802410801638
>1k	0.01281984558239537	9.421068922153296
>5k	0.001109777676799163	2.738263504958046
>10k+	7.653639150339055E-5	0.2877983012188788
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	10522	0.14518167785700695	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	10204	0.140793940396588	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	9893	0.13650278835196444	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	9869	0.1361716383549517	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	9598	0.13243240297201603	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	9308	0.12843100717477862	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	9298	0.12829302800935666	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	7959	0.10981761775935357	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	7586	0.10467099488911374	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	7517	0.10371893864770207	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	7340	0.10127670741973303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3797916542197962E-4
2	0.0	0.0	0.0	0.0	1.3797916542197962E-4
3	0.0	0.0	0.0	0.0	1.3797916542197962E-4
4	0.0	0.0	0.0	1.379791654219796E-5	1.5177708196417757E-4
5	0.0	0.0	0.0	1.379791654219796E-5	1.793729150485735E-4
6	0.0	0.0	0.0	1.379791654219796E-5	2.483624977595633E-4
7	1.379791654219796E-5	0.0	0.0	1.379791654219796E-5	2.483624977595633E-4
8	1.379791654219796E-5	0.0	0.0	5.519166616879184E-5	2.6216041430176124E-4
9	1.379791654219796E-5	0.0	0.0	1.2418124887978165E-4	2.7595833084395925E-4
10	1.379791654219796E-5	0.0	0.0	1.793729150485735E-4	2.7595833084395925E-4
11	1.379791654219796E-5	0.0	0.0	1.793729150485735E-4	2.897562473861572E-4
12	1.379791654219796E-5	0.0	0.0	2.0696874813296942E-4	7.864812429052838E-4
13	1.379791654219796E-5	0.0	0.0	2.3456458121736535E-4	0.001007247907580451
14	2.759583308439592E-5	0.0	0.0	2.483624977595633E-4	0.0010210458241226492
15	2.759583308439592E-5	0.0	0.0	3.173520804705531E-4	0.0013521958211354002
16	2.759583308439592E-5	0.0	0.0	3.58745830097147E-4	0.001462579153472984
17	2.759583308439592E-5	0.0	0.0	3.7254374663934494E-4	0.0015039729030995777
18	2.759583308439592E-5	0.0	0.0	4.8292707897692864E-4	0.0016971437346903493
19	1.1038333233758369E-4	0.0	0.0	5.381187451457205E-4	0.0016971437346903493
20	1.1038333233758369E-4	0.0	0.0	6.760979105677001E-4	0.0019593041489921106
21	1.1038333233758369E-4	0.0	0.0	7.17491660194294E-4	0.002124879147498486
22	1.1038333233758369E-4	0.0	0.0	9.382583248694614E-4	0.002152474980582882
23	1.1038333233758369E-4	0.0	0.0	0.0012280145722556185	0.0021938687302094756
24	1.793729150485735E-4	0.0	0.0	0.001752335400859141	0.002207666646751674
25	1.793729150485735E-4	0.0	0.0	0.002138677064040684	0.002207666646751674
26	1.793729150485735E-4	0.0	0.0	0.002566412476848821	0.0023180499790892576
27	1.793729150485735E-4	0.0	0.0	0.0030217437227413537	0.002373241645258049
28	2.0696874813296942E-4	0.0	0.0	0.003573660384429272	0.0024008374783424454
29	2.483624977595633E-4	0.0	0.0	0.004373939543876754	0.0024008374783424454
30	2.7595833084395925E-4	0.0	0.0	0.005532964533421382	0.0024008374783424454
31	2.7595833084395925E-4	0.0	0.0	0.007575056181666681	0.002428433311426841
32	2.7595833084395925E-4	0.0	0.0	0.010113872825431105	0.002511220810680029
33	2.897562473861572E-4	0.0	0.0	0.012749274884990915	0.002525018727222227
34	2.897562473861572E-4	0.0	0.0	0.016212551937082605	0.0026905937257286025
35	3.173520804705531E-4	0.0	0.0	0.019275689409450552	0.0027043916422708003
36	3.4494791355494904E-4	0.0	0.0	0.023111510208181585	0.0027043916422708003
37	3.4494791355494904E-4	0.0	0.0	0.028796251823567143	0.0027181895588129982
38	3.7254374663934494E-4	0.0	0.0	0.03667486216916218	0.002787179141523988
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8445	0.0	28.63797	1
GGTATCA	3125	0.0	24.506056	1
TCAACGC	13210	0.0	18.10227	4
ATCAACG	13450	0.0	17.81197	3
CAACGCA	13700	0.0	17.486933	5
AACGCAG	13725	0.0	17.455082	6
TAGGACG	815	0.0	17.005495	4
TATCAAC	14535	0.0	16.694708	2
CGCAATA	1965	0.0	16.346342	36
ATACCGT	3210	0.0	15.968246	6
GTATTAC	650	0.0	15.912144	1
ACCGTCG	3170	0.0	15.892147	8
TACCGTC	3275	0.0	15.7184925	7
CCGTCGT	3220	0.0	15.577054	9
CGTCGTA	3225	0.0	15.484902	10
TACGAAT	2065	0.0	15.342838	41
GTCCTAT	2280	0.0	15.056844	1
ATACGAA	2175	0.0	14.667531	40
GTCGTAG	3390	0.0	14.666317	11
CAATACG	2205	0.0	14.567752	38
>>END_MODULE