FastQCFastQC Report
Thu 2 Feb 2017
SRR4061991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808553
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10440.1291195506045986No Hit
TATCAACGCAGAGTACTTTTTTTTT9410.11638074436678857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT504.5795205E-617.0910841
CGTTATT551.1305603E-515.5402372
TCCGATA653.3595352E-614.6186268
CCGATAA653.3595352E-614.6186269
ATTTGCG653.3660217E-614.61590916
ACGAACG653.3660217E-614.61590915
GGTATCA4600.014.4489861
GATAACG707.244884E-613.57275611
AATTCCG707.244884E-613.5727565
CGTCGTA707.244884E-613.57275610
CGATAAC707.244884E-613.57275610
AACGAAC707.2495222E-613.57191514
GGGTTAG500.001504360213.2930651
CCGTCGT751.4731377E-512.6694759
ACGGTAT751.4731377E-512.6694759
GTTTTCG604.0870035E-412.66712215
CGAACGA751.4759558E-512.66712116
TTGCGCG751.4787791E-512.66476718
ATAACGA853.9323822E-612.295320512
ACCGTAT550.003061160412.0935918
Produced by FastQC (version 0.11.5)