##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4061991_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 808553 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13764836689741 32.0 32.0 32.0 32.0 32.0 2 30.59429252009454 32.0 32.0 32.0 32.0 32.0 3 30.63443089073938 32.0 32.0 32.0 32.0 32.0 4 30.64369187919654 32.0 32.0 32.0 32.0 32.0 5 30.523745505860468 32.0 32.0 32.0 32.0 32.0 6 34.17245128025003 36.0 36.0 36.0 32.0 36.0 7 34.10294686928377 36.0 36.0 36.0 32.0 36.0 8 34.03315057887362 36.0 36.0 36.0 32.0 36.0 9 34.20367743363762 36.0 36.0 36.0 32.0 36.0 10 33.83304990520102 36.0 36.0 36.0 32.0 36.0 11 34.181699900934134 36.0 36.0 36.0 32.0 36.0 12 33.96591689103868 36.0 36.0 36.0 32.0 36.0 13 34.06722626717111 36.0 36.0 36.0 32.0 36.0 14 33.95833791971584 36.0 36.0 36.0 32.0 36.0 15 33.90203363292202 36.0 36.0 36.0 32.0 36.0 16 33.902367562794275 36.0 36.0 36.0 32.0 36.0 17 33.826347808987165 36.0 36.0 36.0 32.0 36.0 18 33.825569876062545 36.0 36.0 36.0 32.0 36.0 19 33.84241601972907 36.0 36.0 36.0 32.0 36.0 20 33.83031539058046 36.0 36.0 36.0 32.0 36.0 21 33.80927286151928 36.0 36.0 36.0 32.0 36.0 22 33.77640674142573 36.0 36.0 36.0 32.0 36.0 23 33.74013206308059 36.0 36.0 36.0 32.0 36.0 24 33.71873457893298 36.0 36.0 36.0 27.0 36.0 25 33.331281932044035 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 13.0 5 72.0 6 170.0 7 39.0 8 132.0 9 108.0 10 73.0 11 13.0 12 40.0 13 46.0 14 163.0 15 186.0 16 326.0 17 398.0 18 654.0 19 854.0 20 1323.0 21 1945.0 22 3014.0 23 4458.0 24 6237.0 25 8477.0 26 11573.0 27 14773.0 28 18878.0 29 24845.0 30 31968.0 31 42377.0 32 58061.0 33 79953.0 34 165130.0 35 332253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.970384927913365 17.809239110941807 12.017943431440088 25.202432529704737 2 16.98905448024241 18.661554634840147 37.9903531012306 26.359037783686844 3 20.079896110320945 21.64640405664461 27.737307525817823 30.53639230721662 4 13.454585486107417 15.171815801289624 33.87766512091612 37.495933591686835 5 15.433665894649327 35.87752637725914 32.872844784923885 15.815962943167646 6 36.171260756084855 33.420871228950375 16.035523389572926 14.372344625391847 7 31.975782488114106 29.450029714738513 18.8100485340729 19.764139263074483 8 28.604416203808032 33.70766483999495 17.981655692604086 19.706263263592938 9 26.220079223270194 15.58424994830304 18.825866063390997 39.36980476503577 10 18.120999032770623 26.445611614678604 29.282528815633203 26.150860536917577 11 37.02259632295748 21.66145644968714 20.797187969105313 20.51875925825006 12 24.37058256022332 23.715683761160093 27.70811428033582 24.205619398280767 13 29.96942426220088 18.99968173492854 24.664800006934957 26.366093995935618 14 23.358726259141577 19.568609192470287 24.555644436299335 32.5170201120888 15 25.563103369269736 26.784486869074676 21.522456637913418 26.129953123742172 16 26.094030937486068 25.932557667256095 22.906791288368474 25.06662010688936 17 24.528577602807665 26.21575399719877 24.333160372159607 24.922508027833953 18 25.032812948856375 25.125803607690862 25.221394537944153 24.619988905508613 19 25.822829630161525 25.112197006181514 24.56708978607977 24.49788357757719 20 25.886866356024107 24.5257419025784 24.53131267114639 25.05607907025111 21 26.542797225997976 24.22658971559299 24.376629453840497 24.853983604568537 22 26.117715811082192 24.14947119753762 24.639148963566985 25.0936640278132 23 24.82125704326471 24.351994890229047 25.217982234633553 25.60876583187269 24 24.871565697704646 24.76844687957257 25.019992423923565 25.339994998799213 25 25.147931191570272 24.53330494374901 24.767519014419268 25.55124485026145 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 82.0 1 82.0 2 150.0 3 218.0 4 218.0 5 218.0 6 456.5 7 695.0 8 695.0 9 695.0 10 846.0 11 997.0 12 997.0 13 997.0 14 1427.0 15 1857.0 16 1857.0 17 1857.0 18 3092.0 19 4327.0 20 4327.0 21 4327.0 22 6904.0 23 9481.0 24 9481.0 25 9481.0 26 13742.0 27 18003.0 28 18003.0 29 18003.0 30 23250.5 31 28498.0 32 28498.0 33 28498.0 34 36658.5 35 44819.0 36 44819.0 37 44819.0 38 53774.5 39 62730.0 40 62730.0 41 62730.0 42 72576.5 43 82423.0 44 82423.0 45 82423.0 46 93332.5 47 104242.0 48 104242.0 49 104242.0 50 109319.5 51 114397.0 52 114397.0 53 114397.0 54 108611.0 55 102825.0 56 102825.0 57 102825.0 58 94852.5 59 86880.0 60 86880.0 61 86880.0 62 75931.5 63 64983.0 64 64983.0 65 64983.0 66 52675.0 67 40367.0 68 40367.0 69 40367.0 70 30611.0 71 20855.0 72 20855.0 73 20855.0 74 15610.5 75 10366.0 76 10366.0 77 10366.0 78 7944.0 79 5522.0 80 5522.0 81 5522.0 82 3879.0 83 2236.0 84 2236.0 85 2236.0 86 1606.0 87 976.0 88 976.0 89 976.0 90 657.5 91 339.0 92 339.0 93 339.0 94 219.0 95 99.0 96 99.0 97 99.0 98 217.5 99 336.0 100 336.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014841327655700986 2 3.7103319139252466E-4 3 3.7103319139252466E-4 4 0.01236777304641749 5 0.03475344226043314 6 0.0645597753022993 7 0.10797065869522467 8 0.11019685784357983 9 0.11897797670653623 10 0.135056081666879 11 0.14408455599076375 12 0.13604550351059239 13 0.12986161698738363 14 0.12108049812442723 15 0.13703492535430578 16 0.12231727542906896 17 0.12986161698738363 18 0.11687545528864526 19 0.10166309444155175 20 0.09461346380509379 21 0.09486081926602215 22 0.10871272507800972 23 0.0852139562898165 24 0.09238726465673863 25 0.09213990919581029 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 808553.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.9189447216253 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27898076396485 53.41464603967505 2 11.140250537354191 14.464266176391064 3 2.997887218480475 5.838590238546028 4 1.2058564932249252 3.1313172410352736 5 0.6328143925974687 2.0540821286041977 6 0.36021569915532664 1.4030893836795753 7 0.2701483514070667 1.2276422124143498 8 0.1736822202828433 0.9020213166136841 9 0.13587460943498397 0.7938752633083934 >10 0.7054533715852805 8.08699433042215 >50 0.05533436466174041 2.5115730865527883 >100 0.04102160192943277 5.105308437900312 >500 0.002289577773622641 0.9372799784152316 >1k 1.9079814780188675E-4 0.129314166441853 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1044 0.1291195506045986 No Hit TATCAACGCAGAGTACTTTTTTTTT 941 0.11638074436678857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.236777304641749E-4 0.0 0.0 0.0 6 0.0 1.236777304641749E-4 0.0 0.0 0.0 7 0.0 1.236777304641749E-4 0.0 0.0 0.0 8 0.0 1.236777304641749E-4 0.0 0.0 0.0 9 0.0 1.236777304641749E-4 0.0 0.0 0.0 10 0.0 1.236777304641749E-4 0.0 0.0 0.0 11 0.0 1.236777304641749E-4 0.0 0.0 1.236777304641749E-4 12 0.0 1.236777304641749E-4 0.0 0.0 3.7103319139252466E-4 13 0.0 1.236777304641749E-4 0.0 0.0 4.947109218566996E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTAT 50 4.5795205E-6 17.091084 1 CGTTATT 55 1.1305603E-5 15.540237 2 TCCGATA 65 3.3595352E-6 14.618626 8 CCGATAA 65 3.3595352E-6 14.618626 9 ATTTGCG 65 3.3660217E-6 14.615909 16 ACGAACG 65 3.3660217E-6 14.615909 15 GGTATCA 460 0.0 14.448986 1 GATAACG 70 7.244884E-6 13.572756 11 AATTCCG 70 7.244884E-6 13.572756 5 CGTCGTA 70 7.244884E-6 13.572756 10 CGATAAC 70 7.244884E-6 13.572756 10 AACGAAC 70 7.2495222E-6 13.571915 14 GGGTTAG 50 0.0015043602 13.293065 1 CCGTCGT 75 1.4731377E-5 12.669475 9 ACGGTAT 75 1.4731377E-5 12.669475 9 GTTTTCG 60 4.0870035E-4 12.667122 15 CGAACGA 75 1.4759558E-5 12.667121 16 TTGCGCG 75 1.4787791E-5 12.664767 18 ATAACGA 85 3.9323822E-6 12.2953205 12 ACCGTAT 55 0.0030611604 12.093591 8 >>END_MODULE