FastQCFastQC Report
Thu 2 Feb 2017
SRR4061991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808553
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG401.8145243E-533.012761
GGTATCA5350.029.6189231
TATCGAG300.00574520529.33197410
CGTCGTA1650.022.66561510
GTATAGG803.6009933E-622.0085071
ATACCGT2000.021.998986
ACCGTCG1850.021.4044138
GTAGGAC956.8595E-720.8424283
CCGTCGT1800.020.7768149
GTATCAA9700.020.4202631
GTACTAA654.9173826E-420.3155461
CACGACA901.0016884E-519.5546474
GAGTAAT708.099067E-418.8644331
GCATGCG708.1226806E-418.85626813
GGTAGTA600.007406729318.3347512
AACGCTT1801.4551915E-1118.33248330
GTAAACG1801.4551915E-1118.33248327
TAATACC851.4305991E-418.1168064
TACCGTC2250.017.5991847
GTCGTAG2150.017.39454311