##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808553
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227241751622962	32.0	32.0	32.0	32.0	32.0
2	31.37830296838921	32.0	32.0	32.0	32.0	32.0
3	31.547532443760645	32.0	32.0	32.0	32.0	32.0
4	31.629071934678368	32.0	32.0	32.0	32.0	32.0
5	31.59525225928294	32.0	32.0	32.0	32.0	32.0
6	35.22833011565105	36.0	36.0	36.0	36.0	36.0
7	35.23138866592542	36.0	36.0	36.0	36.0	36.0
8	35.17665879664042	36.0	36.0	36.0	36.0	36.0
9	35.2796885300036	36.0	36.0	36.0	36.0	36.0
10	35.15389714712579	36.0	36.0	36.0	36.0	36.0
11	35.27605487828256	36.0	36.0	36.0	36.0	36.0
12	35.201035677314906	36.0	36.0	36.0	36.0	36.0
13	35.237733333498234	36.0	36.0	36.0	36.0	36.0
14	35.19335281669847	36.0	36.0	36.0	36.0	36.0
15	35.17103517023621	36.0	36.0	36.0	36.0	36.0
16	35.18563656309481	36.0	36.0	36.0	36.0	36.0
17	35.163020853302136	36.0	36.0	36.0	36.0	36.0
18	35.15516237030844	36.0	36.0	36.0	36.0	36.0
19	35.14887954160086	36.0	36.0	36.0	36.0	36.0
20	35.15258368962826	36.0	36.0	36.0	36.0	36.0
21	35.1464937981802	36.0	36.0	36.0	36.0	36.0
22	35.12554526419419	36.0	36.0	36.0	36.0	36.0
23	35.09452936294838	36.0	36.0	36.0	36.0	36.0
24	35.07936152608425	36.0	36.0	36.0	36.0	36.0
25	35.06173250238389	36.0	36.0	36.0	36.0	36.0
26	35.02340724726765	36.0	36.0	36.0	36.0	36.0
27	35.00923501613376	36.0	36.0	36.0	36.0	36.0
28	34.99179769291562	36.0	36.0	36.0	36.0	36.0
29	34.960603695737944	36.0	36.0	36.0	36.0	36.0
30	34.95020610893782	36.0	36.0	36.0	36.0	36.0
31	34.9467369485983	36.0	36.0	36.0	36.0	36.0
32	34.916463113735276	36.0	36.0	36.0	32.0	36.0
33	34.89836906176837	36.0	36.0	36.0	32.0	36.0
34	34.88802589316965	36.0	36.0	36.0	32.0	36.0
35	34.8835895729779	36.0	36.0	36.0	32.0	36.0
36	34.85652270166582	36.0	36.0	36.0	32.0	36.0
37	34.84589260073242	36.0	36.0	36.0	32.0	36.0
38	34.829548588651576	36.0	36.0	36.0	32.0	36.0
39	34.81264431645174	36.0	36.0	36.0	32.0	36.0
40	34.800929561822166	36.0	36.0	36.0	32.0	36.0
41	34.77583411353368	36.0	36.0	36.0	32.0	36.0
42	34.74613909044923	36.0	36.0	36.0	32.0	36.0
43	34.7396027223942	36.0	36.0	36.0	32.0	36.0
44	34.70534646461024	36.0	36.0	36.0	32.0	36.0
45	34.67900805513059	36.0	36.0	36.0	32.0	36.0
46	34.67029495901939	36.0	36.0	36.0	32.0	36.0
47	34.64623221978027	36.0	36.0	36.0	32.0	36.0
48	34.61787044263023	36.0	36.0	36.0	32.0	36.0
49	34.60621505331129	36.0	36.0	36.0	32.0	36.0
50	34.18831913306858	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	20.0
22	43.0
23	149.0
24	381.0
25	951.0
26	1929.0
27	3634.0
28	6389.0
29	9828.0
30	15182.0
31	23095.0
32	35207.0
33	60493.0
34	138485.0
35	512759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.848178065023774	18.350119821004615	12.358327281455024	25.443374832516586
2	16.372692225395387	19.05698952526663	37.77100543340461	26.79931281593337
3	19.45650654816603	22.023333213777306	28.023479154582233	30.49668108347443
4	13.253305596540981	15.607511195926548	33.891037445906456	37.24814576162602
5	15.141617185268002	36.317965550804956	32.635708481695076	15.904708782231964
6	36.31512096300428	33.59000584995665	15.869213273588745	14.225659913450325
7	32.00495205632778	29.402154218709224	18.791470688996267	19.80142303596672
8	28.61346133153918	33.965986150567744	17.803161944857045	19.61739057303603
9	26.393322391976774	15.237467426377737	18.663340560235383	39.7058696214101
10	18.090712668186253	26.37724428701644	29.387312891053526	26.14473015374379
11	37.459511002989295	21.500260341622628	20.691407984386924	20.348820671001157
12	24.363152446407348	23.520659746485386	27.824026377986357	24.292161429120913
13	30.02474791386588	18.846754634513754	24.749645354107894	26.378852097512468
14	23.551702856831895	19.201586043215475	24.52356246281938	32.72314863713325
15	25.60265066111931	26.72626284238634	21.51720419069622	26.153882305798138
16	26.284368665349906	25.80672290733153	22.782787437743416	25.126120989575153
17	24.628441178253002	26.120365640842348	24.382940883281616	24.868252297623037
18	25.130109133364336	25.073340984871724	25.264918026298865	24.53163185546508
19	25.947340495922965	24.969544358873197	24.617186504780765	24.465928640423076
20	26.08184270379976	24.28950060045687	24.641735648091462	24.98692104765191
21	26.65378769233433	24.108252643920682	24.433401397311	24.804558266433986
22	26.189996203093674	23.910491952908465	24.841785263303702	25.057726580694155
23	24.96224737277581	24.13150405724795	25.31707878147753	25.58916978849871
24	24.97823258483108	24.572690798814666	25.089790587571798	25.359286028782456
25	25.29308675169965	24.260867306743314	24.909560212182562	25.53648572937447
26	24.985777043405992	25.055036658124646	25.15806033501865	24.801125963450712
27	25.429319507709508	24.52980469904656	24.81302880352039	25.227846989723542
28	25.164338410354404	24.5162606904996	25.062426952117693	25.256973947028307
29	24.896202951212604	24.844134369098224	25.269835223344533	24.98982745634464
30	24.995825824971586	24.81661457708495	25.147951314896055	25.03960828304741
31	25.408510069903084	24.539668640575453	24.66668645522591	25.385134834295553
32	25.330378223545313	24.524731523246135	24.631219366193264	25.513670887015284
33	24.881733170243635	24.42758854397918	24.937264471222047	25.753413814555138
34	25.64699392861516	24.416144021312306	25.099592231496747	24.83726981857579
35	25.69244411903298	24.466791746696863	25.187712804047745	24.65305133022241
36	24.816833054645837	24.935192789084685	24.861480770562686	25.386493385706792
37	25.78908051094818	24.534467234606748	24.73804505921814	24.938407195226926
38	25.104476762809615	24.43315404185007	25.11536040309046	25.34700879224986
39	25.591278160151877	24.430674854213432	24.631529475786753	25.34651750984794
40	25.762011301698966	24.577052035060248	24.76071391909725	24.900222744143534
41	24.859098751460955	24.98586967948599	25.217891397510346	24.93714017154271
42	25.826520389358205	24.75714586090463	24.80847484879593	24.60785890094124
43	25.582793251867606	24.258769108989263	24.81175481126008	25.346682827883043
44	25.007946667656135	24.735348138253464	24.862742187838197	25.3939630062522
45	25.10379884606109	24.843451427581982	24.878947726443506	25.173801999913426
46	25.24504317088646	24.662876253353303	24.659536863768984	25.43254371199125
47	25.20626659625177	24.737892079684272	25.14046906712214	24.915372256941826
48	25.875522716281786	25.041402963697735	24.258982804319512	24.824091515700967
49	25.27754140858449	25.01484178655532	24.355124374171332	25.352492430688855
50	25.218727042008947	25.212419391942532	24.550363493795004	25.018490072253513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	11.5
2	15.0
3	45.5
4	76.0
5	80.0
6	84.0
7	66.5
8	49.0
9	68.0
10	87.0
11	185.0
12	283.0
13	563.5
14	844.0
15	995.5
16	1147.0
17	1277.5
18	1408.0
19	1295.0
20	1182.0
21	1441.5
22	1701.0
23	2066.0
24	2431.0
25	3267.5
26	4104.0
27	5004.5
28	5905.0
29	7009.5
30	8114.0
31	9524.0
32	10934.0
33	12512.5
34	14091.0
35	16535.5
36	18980.0
37	22485.0
38	25990.0
39	29101.0
40	32212.0
41	35982.0
42	39752.0
43	42268.5
44	44785.0
45	48466.5
46	52148.0
47	55991.5
48	59835.0
49	62750.5
50	65666.0
51	64853.5
52	64041.0
53	64041.0
54	64041.0
55	63598.0
56	63155.0
57	60531.5
58	57908.0
59	53354.0
60	48800.0
61	42836.5
62	36873.0
63	31720.5
64	26568.0
65	22439.5
66	18311.0
67	15520.5
68	12730.0
69	11131.5
70	9533.0
71	7430.5
72	5328.0
73	4652.0
74	3976.0
75	2938.0
76	1900.0
77	1629.5
78	1359.0
79	1147.0
80	935.0
81	756.5
82	578.0
83	463.5
84	349.0
85	271.5
86	194.0
87	134.5
88	75.0
89	51.0
90	27.0
91	18.5
92	10.0
93	10.5
94	11.0
95	10.5
96	10.0
97	6.5
98	3.0
99	5.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032650920842542175
2	0.004452398296710296
3	7.420663827850493E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.947109218566995E-4
17	0.0
18	1.2367773046417487E-4
19	0.0
20	2.4735546092834973E-4
21	0.0
22	0.0
23	0.0
24	6.183886523208744E-4
25	4.947109218566995E-4
26	1.2367773046417487E-4
27	7.420663827850493E-4
28	9.89421843713399E-4
29	4.947109218566995E-4
30	0.0012367773046417488
31	6.183886523208744E-4
32	0.0012367773046417488
33	0.0
34	7.420663827850493E-4
35	4.947109218566995E-4
36	1.2367773046417487E-4
37	0.002102521417890973
38	0.0
39	9.89421843713399E-4
40	2.4735546092834973E-4
41	9.89421843713399E-4
42	0.00531814240995952
43	0.004081365105317772
44	0.0046997537576386455
45	0.002226199148355148
46	0.0027209100702118476
47	0.0014841327655700986
48	0.004452398296710296
49	0.0030919432616043725
50	0.0013604550351059238
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	808553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.83588821319111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85367590753103	53.88909456886986
2	11.625901410525204	15.308030912818364
3	2.9385588888369782	5.803879035400511
4	1.2529295777970024	3.299509264913767
5	0.6226387792919285	2.049598853533058
6	0.3805833846719433	1.503362710143598
7	0.25453236612776614	1.173015508211865
8	0.1828636878678892	0.9631194650177779
9	0.13278406269626392	0.7867761037348131
>10	0.674209376020264	7.902114929173642
>50	0.04415288319670014	2.0622241680479254
>100	0.03528288989049102	4.471731077424444
>500	0.0018867855464789955	0.7875434027104219
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.236777304641749E-4
2	0.0	0.0	0.0	0.0	1.236777304641749E-4
3	0.0	0.0	0.0	0.0	1.236777304641749E-4
4	0.0	0.0	0.0	0.0	1.236777304641749E-4
5	0.0	0.0	0.0	0.0	1.236777304641749E-4
6	0.0	0.0	0.0	0.0	1.236777304641749E-4
7	0.0	0.0	0.0	0.0	1.236777304641749E-4
8	0.0	0.0	0.0	0.0	1.236777304641749E-4
9	0.0	0.0	0.0	0.0	1.236777304641749E-4
10	0.0	0.0	0.0	0.0	2.473554609283498E-4
11	0.0	0.0	0.0	0.0	2.473554609283498E-4
12	0.0	0.0	0.0	0.0	4.947109218566996E-4
13	0.0	0.0	0.0	0.0	7.420663827850493E-4
14	0.0	0.0	0.0	0.0	7.420663827850493E-4
15	0.0	0.0	0.0	0.0	9.894218437133992E-4
16	0.0	0.0	0.0	1.236777304641749E-4	0.001236777304641749
17	0.0	0.0	0.0	2.473554609283498E-4	0.001236777304641749
18	0.0	0.0	0.0	4.947109218566996E-4	0.001236777304641749
19	0.0	0.0	0.0	4.947109218566996E-4	0.001236777304641749
20	0.0	1.236777304641749E-4	0.0	7.420663827850493E-4	0.001236777304641749
21	0.0	1.236777304641749E-4	0.0	0.001113099574177574	0.001236777304641749
22	0.0	1.236777304641749E-4	0.0	0.001236777304641749	0.0013604550351059238
23	0.0	1.236777304641749E-4	0.0	0.0019788436874267983	0.0014841327655700986
24	0.0	1.236777304641749E-4	0.0	0.0023498768788193227	0.0014841327655700986
25	0.0	1.236777304641749E-4	0.0	0.003462976452996897	0.0014841327655700986
26	0.0	1.236777304641749E-4	0.0	0.004081365105317771	0.0016078104960342737
27	0.0	1.236777304641749E-4	0.0	0.004823431488102821	0.0016078104960342737
28	0.0	1.236777304641749E-4	0.0	0.005441820140423695	0.0016078104960342737
29	0.0	1.236777304641749E-4	0.0	0.006183886523208744	0.0016078104960342737
30	0.0	1.236777304641749E-4	0.0	0.007668019288778844	0.0016078104960342737
31	0.0	1.236777304641749E-4	0.0	0.009399507515277291	0.0016078104960342737
32	0.0	1.236777304641749E-4	0.0	0.011502028933168264	0.0017314882264984485
33	0.0	1.236777304641749E-4	0.0	0.012738806237810013	0.0018551659569626233
34	0.0	1.236777304641749E-4	0.0	0.015088683116629337	0.0019788436874267983
35	0.0	1.236777304641749E-4	0.0	0.017191204534520308	0.0019788436874267983
36	0.0	1.236777304641749E-4	0.0	0.020901536448445557	0.0019788436874267983
37	0.0	1.236777304641749E-4	0.0	0.024488190631906628	0.0019788436874267983
38	0.0	1.236777304641749E-4	0.0	0.030548399424651197	0.0019788436874267983
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	40	1.8145243E-5	33.01276	1
GGTATCA	535	0.0	29.618923	1
TATCGAG	30	0.005745205	29.331974	10
CGTCGTA	165	0.0	22.665615	10
GTATAGG	80	3.6009933E-6	22.008507	1
ATACCGT	200	0.0	21.99898	6
ACCGTCG	185	0.0	21.404413	8
GTAGGAC	95	6.8595E-7	20.842428	3
CCGTCGT	180	0.0	20.776814	9
GTATCAA	970	0.0	20.420263	1
GTACTAA	65	4.9173826E-4	20.315546	1
CACGACA	90	1.0016884E-5	19.554647	4
GAGTAAT	70	8.099067E-4	18.864433	1
GCATGCG	70	8.1226806E-4	18.856268	13
GGTAGTA	60	0.0074067293	18.334751	2
AACGCTT	180	1.4551915E-11	18.332483	30
GTAAACG	180	1.4551915E-11	18.332483	27
TAATACC	85	1.4305991E-4	18.116806	4
TACCGTC	225	0.0	17.599184	7
GTCGTAG	215	0.0	17.394543	11
>>END_MODULE