FastQCFastQC Report
Thu 2 Feb 2017
SRR4061990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761735
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34770.12589911776473847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.013.2371821
AACCGCG2100.013.1202747
CGTCGTA3800.012.25181310
GTATCAA28200.012.1902071
CGTTATT1850.011.8093462
CCGTCGT3950.011.5458039
GCGTTAT2000.011.3954871
ACCGTCG3950.011.3052658
CGCGGTC2300.011.15382510
GACCCGT600.005873523711.0843716
CGACCAT4200.010.85874910
TACCGTC4450.010.6751567
ATACCGT4550.010.6493486
CGGTCCA3400.010.61921910
CGCCGGT3600.010.5565437
AGAACCG2950.010.3058615
ACGAACG1303.8472717E-710.23024215
TCTTGCG3700.010.0122722
ACTCTAA2950.09.98452810
TAGAACC3250.09.938674
Produced by FastQC (version 0.11.5)