##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061990_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2761735
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253402299641348	32.0	32.0	32.0	32.0	32.0
2	30.762431225298588	32.0	32.0	32.0	32.0	32.0
3	30.793089851126194	32.0	32.0	32.0	32.0	32.0
4	30.802026262476307	32.0	32.0	32.0	32.0	32.0
5	30.703018573469215	32.0	32.0	32.0	32.0	32.0
6	34.36032856157452	36.0	36.0	36.0	32.0	36.0
7	34.29648065437126	36.0	36.0	36.0	32.0	36.0
8	34.24615214711042	36.0	36.0	36.0	32.0	36.0
9	34.394020787657034	36.0	36.0	36.0	32.0	36.0
10	34.06423027553331	36.0	36.0	36.0	32.0	36.0
11	34.37981341439349	36.0	36.0	36.0	32.0	36.0
12	34.19138295310738	36.0	36.0	36.0	32.0	36.0
13	34.28651735231657	36.0	36.0	36.0	32.0	36.0
14	34.184151267228756	36.0	36.0	36.0	32.0	36.0
15	34.1331800480495	36.0	36.0	36.0	32.0	36.0
16	34.13964229008214	36.0	36.0	36.0	32.0	36.0
17	34.07207968903606	36.0	36.0	36.0	32.0	36.0
18	34.073394080170615	36.0	36.0	36.0	32.0	36.0
19	34.08635911845271	36.0	36.0	36.0	32.0	36.0
20	34.07412695280322	36.0	36.0	36.0	32.0	36.0
21	34.045065873445495	36.0	36.0	36.0	32.0	36.0
22	34.018184945333275	36.0	36.0	36.0	32.0	36.0
23	33.98828707316234	36.0	36.0	36.0	32.0	36.0
24	33.96077176122981	36.0	36.0	36.0	32.0	36.0
25	33.59513494234603	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	39.0
5	226.0
6	554.0
7	145.0
8	501.0
9	386.0
10	195.0
11	53.0
12	123.0
13	99.0
14	294.0
15	491.0
16	808.0
17	1161.0
18	1598.0
19	2323.0
20	3575.0
21	5278.0
22	7909.0
23	11719.0
24	16764.0
25	23471.0
26	32701.0
27	42379.0
28	56281.0
29	75599.0
30	98873.0
31	133811.0
32	189125.0
33	267228.0
34	576807.0
35	1211217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.800894809222996	17.581270417633156	11.980565635878692	25.637269137265157
2	16.70258015902357	19.48938509173058	37.65018218659398	26.157852562651872
3	19.218442729649706	23.061812633227806	28.13094172936619	29.588802907756296
4	13.014237111367418	15.813206491528891	35.31415617548059	35.8584002216231
5	15.18045040339552	36.20240262473314	33.20017212314919	15.41697484872215
6	34.59655916674487	34.68873404829254	16.861698909155205	13.853007875807391
7	30.79969580690553	30.119429862685383	20.13963448981323	18.94123984059586
8	28.15510331025628	33.18412210840572	19.01901201604072	19.64176256529728
9	26.891319889038893	15.129145271754657	18.694633641284724	39.28490119792173
10	16.573196049724338	26.868741583837487	30.900595557934018	25.65746680850416
11	36.600894106504484	21.58089836346383	21.978202482597776	19.840005047433912
12	24.49933830061094	23.831910226428093	28.418411558891243	23.250339914069723
13	29.2353619796142	19.76101028426572	24.96938213918238	26.034245596937705
14	23.462923248027586	19.730089887868544	25.145910094730993	31.661076769372876
15	25.17665605648643	27.14134623716889	22.006981036916393	25.675016669428285
16	25.703351974344862	26.066529052353616	23.4307581765596	24.799360796741922
17	24.14090888821456	26.25797911969468	24.89351203806122	24.70759995402954
18	24.657557585461525	25.53868048700827	25.6326761131123	24.171085814417907
19	25.43715592489671	25.416532712438116	25.078841200176726	24.06747016248844
20	25.616838860984153	24.86016724479548	24.805151078813374	24.71784281540699
21	26.28215629281163	24.576069701867947	24.578534202190504	24.563239803129918
22	25.688908736084816	24.59860173927164	24.86639389660062	24.846095628042928
23	24.456222771276185	24.70018054034544	25.28072175551025	25.56287493286813
24	24.49800230213658	25.318428400363008	25.184729064039406	24.998840233461003
25	24.74553088964706	24.859111490921162	25.107292715402675	25.2880649040291
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	307.0
1	307.0
2	528.5
3	750.0
4	750.0
5	750.0
6	1616.5
7	2483.0
8	2483.0
9	2483.0
10	3122.5
11	3762.0
12	3762.0
13	3762.0
14	5626.5
15	7491.0
16	7491.0
17	7491.0
18	12671.5
19	17852.0
20	17852.0
21	17852.0
22	28038.0
23	38224.0
24	38224.0
25	38224.0
26	56775.0
27	75326.0
28	75326.0
29	75326.0
30	95276.5
31	115227.0
32	115227.0
33	115227.0
34	143108.5
35	170990.0
36	170990.0
37	170990.0
38	199781.5
39	228573.0
40	228573.0
41	228573.0
42	260532.5
43	292492.0
44	292492.0
45	292492.0
46	323718.0
47	354944.0
48	354944.0
49	354944.0
50	367014.0
51	379084.0
52	379084.0
53	379084.0
54	357224.5
55	335365.0
56	335365.0
57	335365.0
58	307544.5
59	279724.0
60	279724.0
61	279724.0
62	242872.0
63	206020.0
64	206020.0
65	206020.0
66	166413.0
67	126806.0
68	126806.0
69	126806.0
70	95484.5
71	64163.0
72	64163.0
73	64163.0
74	47872.0
75	31581.0
76	31581.0
77	31581.0
78	24808.5
79	18036.0
80	18036.0
81	18036.0
82	12494.0
83	6952.0
84	6952.0
85	6952.0
86	5071.5
87	3191.0
88	3191.0
89	3191.0
90	2145.5
91	1100.0
92	1100.0
93	1100.0
94	697.5
95	295.0
96	295.0
97	295.0
98	646.0
99	997.0
100	997.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010500645427602576
2	2.1725473298488088E-4
3	6.517641989546427E-4
4	0.010500645427602576
5	0.03334860151317922
6	0.06387289149755498
7	0.1072152107280387
8	0.10884462122542533
9	0.11916422104220716
10	0.13212708677697171
11	0.14114315819584428
12	0.133430615174881
13	0.12821650158324388
14	0.11539847233713589
15	0.13368407903003005
16	0.11952631226384863
17	0.12463179848899333
18	0.11210344222019854
19	0.09805430282050957
20	0.09218842502991778
21	0.09251430712939511
22	0.10424606271057868
23	0.081144642769853
24	0.09265914361805169
25	0.08911064964596531
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2761735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.70070000598154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25056964118028	36.63154456638758
2	15.506181254675896	15.72348488063394
3	5.4541776528557016	8.295918748702961
4	2.3848733224984304	4.836589875050455
5	1.2532111924104454	3.1769342355270203
6	0.7228400345595796	2.198909744671114
7	0.4960745893366465	1.7605930254183457
8	0.3346415931861825	1.357325042052508
9	0.2339149522261964	1.0673686637760498
>10	1.2045430691731065	10.95238823473578
>50	0.0878893647391608	3.0954222907577646
>100	0.061203542572541805	6.292405195607756
>500	0.006872897798857287	2.3698596423345033
>1k	0.0030068927869648923	2.241255854344151
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3477	0.12589911776473847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4483648865658725E-4
2	0.0	0.0	0.0	0.0	1.4483648865658725E-4
3	0.0	0.0	0.0	0.0	1.4483648865658725E-4
4	0.0	0.0	0.0	0.0	1.4483648865658725E-4
5	3.620912216414681E-5	0.0	0.0	0.0	1.4483648865658725E-4
6	3.620912216414681E-5	0.0	0.0	0.0	2.1725473298488088E-4
7	7.241824432829362E-5	0.0	0.0	3.620912216414681E-5	2.1725473298488088E-4
8	7.241824432829362E-5	0.0	0.0	3.620912216414681E-5	2.1725473298488088E-4
9	7.241824432829362E-5	0.0	0.0	3.620912216414681E-5	2.1725473298488088E-4
10	7.241824432829362E-5	0.0	0.0	3.620912216414681E-5	2.1725473298488088E-4
11	1.0862736649244044E-4	0.0	0.0	3.620912216414681E-5	2.1725473298488088E-4
12	1.0862736649244044E-4	0.0	0.0	3.620912216414681E-5	2.534638551490277E-4
13	1.0862736649244044E-4	0.0	0.0	3.620912216414681E-5	4.3450946596976177E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1155	0.0	13.237182	1
AACCGCG	210	0.0	13.120274	7
CGTCGTA	380	0.0	12.251813	10
GTATCAA	2820	0.0	12.190207	1
CGTTATT	185	0.0	11.809346	2
CCGTCGT	395	0.0	11.545803	9
GCGTTAT	200	0.0	11.395487	1
ACCGTCG	395	0.0	11.305265	8
CGCGGTC	230	0.0	11.153825	10
GACCCGT	60	0.0058735237	11.084371	6
CGACCAT	420	0.0	10.858749	10
TACCGTC	445	0.0	10.675156	7
ATACCGT	455	0.0	10.649348	6
CGGTCCA	340	0.0	10.619219	10
CGCCGGT	360	0.0	10.556543	7
AGAACCG	295	0.0	10.305861	5
ACGAACG	130	3.8472717E-7	10.230242	15
TCTTGCG	370	0.0	10.012272	2
ACTCTAA	295	0.0	9.984528	10
TAGAACC	325	0.0	9.93867	4
>>END_MODULE