FastQCFastQC Report
Thu 2 Feb 2017
SRR4061990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761735
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.024.8245071
GGTATCA15550.023.9173411
TCGATCG1158.780262E-921.04424941
CGCAATA4200.019.90513236
GTGTACG1751.9099389E-1017.6054271
CAATACG4550.017.40691838
CGCCGGT5000.017.1593867
TCAACGC45450.016.8442824
CGATCGT1451.2499004E-716.6908742
TATCAAC46250.016.6010882
ATCAACG46100.016.5590613
CAACGCA46500.016.5112385
ACCGTCG6400.016.499418
TACGAAT4850.016.33049641
ATACCGT7150.016.3071086
GCGCAAT5150.016.23301735
ATACGAA4750.016.2111240
AACGCAG48350.015.9704726
TACCGTC6750.015.9697997
CTAGCGG5100.015.96050129
Produced by FastQC (version 0.11.5)