##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2761735
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272110466789897	32.0	32.0	32.0	32.0	32.0
2	31.418226223732546	32.0	32.0	32.0	32.0	32.0
3	31.56001607685024	32.0	32.0	32.0	32.0	32.0
4	31.64232448080645	32.0	32.0	32.0	32.0	32.0
5	31.608837560446602	32.0	32.0	32.0	32.0	32.0
6	35.251632759841186	36.0	36.0	36.0	36.0	36.0
7	35.25588045196226	36.0	36.0	36.0	36.0	36.0
8	35.20503161961593	36.0	36.0	36.0	36.0	36.0
9	35.29906381314645	36.0	36.0	36.0	36.0	36.0
10	35.17503815536248	36.0	36.0	36.0	36.0	36.0
11	35.2971472643103	36.0	36.0	36.0	36.0	36.0
12	35.21994144984946	36.0	36.0	36.0	36.0	36.0
13	35.25988228414384	36.0	36.0	36.0	36.0	36.0
14	35.217599443827886	36.0	36.0	36.0	36.0	36.0
15	35.192886717950856	36.0	36.0	36.0	36.0	36.0
16	35.208799540868334	36.0	36.0	36.0	36.0	36.0
17	35.19031297354743	36.0	36.0	36.0	36.0	36.0
18	35.18302987071533	36.0	36.0	36.0	36.0	36.0
19	35.17640323926807	36.0	36.0	36.0	36.0	36.0
20	35.17806197915441	36.0	36.0	36.0	36.0	36.0
21	35.17325268354857	36.0	36.0	36.0	36.0	36.0
22	35.151790812659435	36.0	36.0	36.0	36.0	36.0
23	35.120785303441494	36.0	36.0	36.0	36.0	36.0
24	35.10768737768106	36.0	36.0	36.0	36.0	36.0
25	35.09285829378995	36.0	36.0	36.0	36.0	36.0
26	35.047723260921124	36.0	36.0	36.0	36.0	36.0
27	35.035753973498544	36.0	36.0	36.0	36.0	36.0
28	35.017735952218445	36.0	36.0	36.0	36.0	36.0
29	34.997941149313746	36.0	36.0	36.0	36.0	36.0
30	34.984089349629855	36.0	36.0	36.0	36.0	36.0
31	34.979951009057714	36.0	36.0	36.0	36.0	36.0
32	34.955808938945985	36.0	36.0	36.0	36.0	36.0
33	34.935065819131815	36.0	36.0	36.0	36.0	36.0
34	34.92574197017455	36.0	36.0	36.0	36.0	36.0
35	34.91297282324336	36.0	36.0	36.0	36.0	36.0
36	34.89148633015115	36.0	36.0	36.0	32.0	36.0
37	34.880357094362786	36.0	36.0	36.0	32.0	36.0
38	34.86624893409397	36.0	36.0	36.0	32.0	36.0
39	34.852130273179725	36.0	36.0	36.0	32.0	36.0
40	34.83906891863267	36.0	36.0	36.0	32.0	36.0
41	34.81409657334972	36.0	36.0	36.0	32.0	36.0
42	34.79065985693776	36.0	36.0	36.0	32.0	36.0
43	34.787207679230626	36.0	36.0	36.0	32.0	36.0
44	34.74157513302326	36.0	36.0	36.0	32.0	36.0
45	34.72836206225434	36.0	36.0	36.0	32.0	36.0
46	34.71421407195115	36.0	36.0	36.0	32.0	36.0
47	34.68910702873375	36.0	36.0	36.0	32.0	36.0
48	34.67321556919835	36.0	36.0	36.0	32.0	36.0
49	34.658342310178206	36.0	36.0	36.0	32.0	36.0
50	34.247930739191126	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	24.0
21	63.0
22	175.0
23	506.0
24	1282.0
25	2940.0
26	6454.0
27	12199.0
28	20953.0
29	32869.0
30	50299.0
31	75404.0
32	115041.0
33	198065.0
34	459785.0
35	1785666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54014162305087	18.120071717043665	12.353296991868447	25.986489668037017
2	16.19507599006522	20.013354523863065	37.22146240002665	26.570107086045063
3	18.59782194651762	23.469619789320614	28.48495074829853	29.447607515863233
4	12.718997296989029	16.307502349066798	35.35849746626668	35.61500288767749
5	14.877169605338672	36.54467209924196	33.02166934915913	15.556488946260231
6	34.7171046533185	34.821713072395255	16.79227642982944	13.668905844456805
7	30.8120800873364	30.181787897825096	20.081325688380673	18.924806326457823
8	28.169936652140777	33.241169047718195	18.97271099508099	19.616183305060044
9	26.972211309195128	14.900777952989696	18.618404734704814	39.508606003110366
10	16.563223164585423	26.872438429058853	30.912655205988415	25.6516832003673
11	36.82601697845738	21.42931888830753	21.995991650176432	19.74867248305866
12	24.529009672908398	23.74211274510869	28.46363914844804	23.265238433534876
13	29.42378613444085	19.63475858473025	25.002254017854717	25.939201262974183
14	23.522532031494695	19.616617814526013	25.196226285288052	31.664623868691237
15	25.262597606214932	27.069360383961534	21.952214821479977	25.71582718834356
16	25.742010682477108	25.999929753896033	23.43500551649172	24.823054047135134
17	24.207644831962515	26.236912665407797	24.815233901876898	24.740208600752787
18	24.717405394444786	25.465088911660445	25.621512602607787	24.195993091286983
19	25.48991847516145	25.353772175824258	25.115950661450135	24.040358687564158
20	25.78559624350587	24.66361591527611	24.852048937547703	24.698738903670318
21	26.43150048791792	24.559850963253172	24.53573568789185	24.472912860937058
22	25.83781572091457	24.50184394954621	24.892721423308174	24.76761890623105
23	24.568097121661335	24.521387303329938	25.349164944317554	25.561350630691177
24	24.627514197694477	25.165223361127648	25.199984357551607	25.007278083626268
25	24.90100400908417	24.63616981831665	25.172898996570265	25.28992717602892
26	24.748300428174417	25.36081615656881	25.408069232092263	24.482814183164507
27	25.214557852589287	25.084494261921712	24.75969737765441	24.941250507834592
28	24.80775351605485	24.892411853760507	25.30183521750639	24.997999412678258
29	24.79491969324947	25.124424136671202	25.28088443337728	24.799771736702052
30	24.799081723361923	25.109869120232318	25.37003543113921	24.72101372526655
31	25.150132725113995	24.94652794650296	24.83170832121831	25.07163100716473
32	25.074917406184866	24.943295960326	24.71810922805742	25.26367740543171
33	24.621333504240635	24.78724394229561	25.177615053747086	25.41380749971666
34	25.159900815146546	25.009088565353665	25.288408059928045	24.542602559571744
35	25.5335889949861	24.703201588283836	25.236781530950065	24.52642788578
36	24.687179661726404	25.233648920658037	25.114303394721205	24.964868022894354
37	25.487672050322775	24.956548376864617	24.85968746695866	24.696092105853946
38	24.828703695321963	24.87791189234304	25.258505975410383	25.03487843692461
39	25.307643982090838	24.94598465084196	24.7092476957253	25.037123671341906
40	25.475462499619805	24.967303127168023	25.021616869413833	24.535617503798342
41	24.66005601612048	25.41209144351044	25.24911241425134	24.67874012611774
42	25.54003999853708	25.171666725207587	24.892806745072875	24.395486531182456
43	25.100438907615757	24.62213425481844	25.075200180762874	25.202226656802925
44	24.849762313769	25.10939215476111	24.916860513958454	25.123985017511437
45	25.013524386260816	25.185050538114872	24.87896851117328	24.922456564451036
46	24.885857232192226	25.10413072076238	24.963599962776186	25.04641208426921
47	24.9448347432127	25.09282319381309	25.225060488061345	24.737281574912863
48	25.435321671646804	25.461863909653555	24.461876945404825	24.640937473294816
49	24.91232478989634	25.438249312992077	24.61036358099415	25.039062316117427
50	25.070355419793373	25.485571888537894	24.712422674215663	24.73165001745307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	87.0
1	67.5
2	48.0
3	115.0
4	182.0
5	212.0
6	242.0
7	219.0
8	196.0
9	273.0
10	350.0
11	579.0
12	808.0
13	1484.5
14	2161.0
15	2745.5
16	3330.0
17	3630.0
18	3930.0
19	4160.5
20	4391.0
21	5499.0
22	6607.0
23	8344.5
24	10082.0
25	13451.5
26	16821.0
27	21244.0
28	25667.0
29	30682.0
30	35697.0
31	40618.0
32	45539.0
33	52386.5
34	59234.0
35	68172.5
36	77111.0
37	88853.0
38	100595.0
39	110913.0
40	121231.0
41	133253.5
42	145276.0
43	151427.0
44	157578.0
45	170461.0
46	183344.0
47	193206.5
48	203069.0
49	209805.0
50	216541.0
51	212077.5
52	207614.0
53	207716.0
54	207818.0
55	205570.0
56	203322.0
57	196599.5
58	189877.0
59	172994.0
60	156111.0
61	137294.5
62	118478.0
63	101302.0
64	84126.0
65	70794.0
66	57462.0
67	48747.5
68	40033.0
69	35065.0
70	30097.0
71	23449.5
72	16802.0
73	14562.0
74	12322.0
75	9151.0
76	5980.0
77	5263.5
78	4547.0
79	3840.5
80	3134.0
81	2414.0
82	1694.0
83	1409.5
84	1125.0
85	881.5
86	638.0
87	419.5
88	201.0
89	144.0
90	87.0
91	62.0
92	37.0
93	43.0
94	49.0
95	38.5
96	28.0
97	27.0
98	26.0
99	18.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03204507311526993
2	0.004489931148354205
3	1.0862736649244044E-4
4	0.0
5	0.0
6	2.534638551490277E-4
7	0.0
8	0.0
9	0.0
10	7.241824432829362E-5
11	0.0
12	3.620912216414681E-5
13	0.0
14	0.0
15	0.0
16	5.79345954626349E-4
17	0.0
18	1.8104561082073407E-4
19	0.0
20	3.98300343805615E-4
21	0.0
22	0.0
23	1.0862736649244044E-4
24	6.879733211187895E-4
25	2.534638551490277E-4
26	3.6209122164146813E-4
27	3.2588209947732134E-4
28	0.0016656196195507535
29	4.3450946596976177E-4
30	0.0014483648865658725
31	4.3450946596976177E-4
32	9.77646298431964E-4
33	1.4483648865658725E-4
34	8.328098097753767E-4
35	4.3450946596976177E-4
36	2.1725473298488088E-4
37	0.0015569922530583131
38	0.0
39	7.241824432829363E-4
40	1.0862736649244044E-4
41	0.0010862736649244044
42	0.004852022369995673
43	0.003838166949399562
44	0.004019212560220297
45	0.0020639199633563686
46	0.0022449655741771024
47	0.0011949010314168448
48	0.0035847030942505343
49	0.004417512904025912
50	0.0015569922530583131
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2761735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.67021517274151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46386011677905	36.92553029308673
2	16.236218134665034	16.7785776921942
3	5.562827539391932	8.622974877877
4	2.403277602639157	4.967114833927823
5	1.258879136647852	3.252327793353478
6	0.730495753727097	2.2646923666711856
7	0.4850502606514967	1.754385593622003
8	0.3146668899374683	1.3007124728645083
9	0.2326607849737382	1.0819469539666795
>10	1.171834925354898	10.755029204269228
>50	0.078757027618839	2.8256533957929437
>100	0.052667436785437824	5.448718900848841
>500	0.0062687262695631935	2.182729039491428
>1k	0.00253566455842505	1.839606582034075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.241824432829362E-5
2	0.0	0.0	0.0	0.0	7.241824432829362E-5
3	0.0	0.0	0.0	0.0	7.241824432829362E-5
4	0.0	0.0	0.0	0.0	7.241824432829362E-5
5	0.0	0.0	0.0	0.0	7.241824432829362E-5
6	0.0	0.0	0.0	0.0	1.4483648865658725E-4
7	0.0	0.0	0.0	3.620912216414681E-5	1.4483648865658725E-4
8	0.0	0.0	0.0	3.620912216414681E-5	1.4483648865658725E-4
9	0.0	0.0	0.0	3.620912216414681E-5	1.4483648865658725E-4
10	0.0	0.0	0.0	3.620912216414681E-5	1.4483648865658725E-4
11	0.0	0.0	0.0	3.620912216414681E-5	1.4483648865658725E-4
12	0.0	0.0	0.0	3.620912216414681E-5	2.1725473298488088E-4
13	0.0	0.0	0.0	3.620912216414681E-5	2.896729773131745E-4
14	0.0	0.0	0.0	7.241824432829362E-5	2.896729773131745E-4
15	0.0	0.0	0.0	1.0862736649244044E-4	3.2588209947732134E-4
16	0.0	0.0	0.0	2.1725473298488088E-4	3.2588209947732134E-4
17	0.0	0.0	0.0	2.1725473298488088E-4	3.6209122164146813E-4
18	0.0	0.0	0.0	2.534638551490277E-4	3.983003438056149E-4
19	7.241824432829362E-5	0.0	0.0	3.2588209947732134E-4	3.983003438056149E-4
20	7.241824432829362E-5	0.0	0.0	4.3450946596976177E-4	5.069277102980554E-4
21	7.241824432829362E-5	0.0	0.0	5.069277102980554E-4	5.79345954626349E-4
22	7.241824432829362E-5	0.0	0.0	7.603915654470831E-4	6.879733211187895E-4
23	7.241824432829362E-5	0.0	0.0	9.414371762678171E-4	6.879733211187895E-4
24	7.241824432829362E-5	0.0	0.0	0.0014483648865658725	7.241824432829363E-4
25	7.241824432829362E-5	0.0	0.0	0.0018466652303714874	7.241824432829363E-4
26	7.241824432829362E-5	3.620912216414681E-5	0.0	0.002534638551490277	8.328098097753767E-4
27	7.241824432829362E-5	3.620912216414681E-5	0.0	0.003150193628280773	8.690189319395235E-4
28	1.0862736649244044E-4	3.620912216414681E-5	0.0	0.0036209122164146813	9.052280541036703E-4
29	2.534638551490277E-4	3.620912216414681E-5	0.0	0.004236467293205177	9.052280541036703E-4
30	2.534638551490277E-4	3.620912216414681E-5	0.0	0.005431368324622022	9.052280541036703E-4
31	2.534638551490277E-4	3.620912216414681E-5	0.0	0.007531497410142537	9.052280541036703E-4
32	2.534638551490277E-4	3.620912216414681E-5	0.0	0.00999371771730452	9.414371762678171E-4
33	2.534638551490277E-4	3.620912216414681E-5	0.0	0.012166265047153329	9.77646298431964E-4
34	2.534638551490277E-4	3.620912216414681E-5	0.0	0.015062994820285074	0.0010138554205961108
35	2.896729773131745E-4	3.620912216414681E-5	0.0	0.01839423405938658	0.0010138554205961108
36	2.896729773131745E-4	3.620912216414681E-5	0.0	0.022196191886621995	0.0010138554205961108
37	2.896729773131745E-4	3.620912216414681E-5	0.0	0.027808605822064753	0.0010138554205961108
38	2.896729773131745E-4	3.620912216414681E-5	0.0	0.03472454815541679	0.0010862736649244044
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3085	0.0	24.824507	1
GGTATCA	1555	0.0	23.917341	1
TCGATCG	115	8.780262E-9	21.044249	41
CGCAATA	420	0.0	19.905132	36
GTGTACG	175	1.9099389E-10	17.605427	1
CAATACG	455	0.0	17.406918	38
CGCCGGT	500	0.0	17.159386	7
TCAACGC	4545	0.0	16.844282	4
CGATCGT	145	1.2499004E-7	16.69087	42
TATCAAC	4625	0.0	16.601088	2
ATCAACG	4610	0.0	16.559061	3
CAACGCA	4650	0.0	16.511238	5
ACCGTCG	640	0.0	16.49941	8
TACGAAT	485	0.0	16.330496	41
ATACCGT	715	0.0	16.307108	6
GCGCAAT	515	0.0	16.233017	35
ATACGAA	475	0.0	16.21112	40
AACGCAG	4835	0.0	15.970472	6
TACCGTC	675	0.0	15.969799	7
CTAGCGG	510	0.0	15.960501	29
>>END_MODULE