FastQCFastQC Report
Thu 2 Feb 2017
SRR4061989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668674
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39220.10690511067486508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.014.6627031
GTATCAA35800.012.5736691
TCGCGTA2700.012.3162489
GCGTAAC3100.011.95316711
ATACCGT5100.011.5500486
TAATACG752.0754061E-411.3999254
CGCGTAA3200.011.28271710
CGTCGTA4850.011.166410
TCGGCGT1752.0008883E-1110.85751713
CGAACGA1408.361894E-910.85736816
CCGTCGT4750.010.8012579
TTAGGAC12850.010.7923783
AACCGCG1252.2398126E-710.6410927
TGTAGGA25850.010.6192272
TGGCGAG20600.010.60614918
TAGGACC16000.010.5686824
CGACGCT909.521555E-510.55563215
TACCGTC5050.010.5357367
ACCGTCG4600.010.533698
AGGACGT19150.010.5174775
Produced by FastQC (version 0.11.5)