##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061989_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3668674
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182897962588118	32.0	32.0	32.0	32.0	32.0
2	30.83301432615708	32.0	32.0	32.0	32.0	32.0
3	30.853059988431788	32.0	32.0	32.0	32.0	32.0
4	30.841190304725902	32.0	32.0	32.0	32.0	32.0
5	30.831383219114045	32.0	32.0	32.0	32.0	32.0
6	34.253607434184666	36.0	36.0	36.0	32.0	36.0
7	34.237941555995434	36.0	36.0	36.0	32.0	36.0
8	34.20798332040405	36.0	36.0	36.0	32.0	36.0
9	34.33968621905353	36.0	36.0	36.0	32.0	36.0
10	34.122463047956835	36.0	36.0	36.0	32.0	36.0
11	34.289758370463005	36.0	36.0	36.0	32.0	36.0
12	34.174114134970836	36.0	36.0	36.0	32.0	36.0
13	34.22738542590593	36.0	36.0	36.0	32.0	36.0
14	34.166100612919	36.0	36.0	36.0	32.0	36.0
15	34.10827427021316	36.0	36.0	36.0	32.0	36.0
16	34.12690416210326	36.0	36.0	36.0	32.0	36.0
17	34.057283094654906	36.0	36.0	36.0	32.0	36.0
18	34.05928708846848	36.0	36.0	36.0	32.0	36.0
19	34.047674718440504	36.0	36.0	36.0	32.0	36.0
20	34.01491110957256	36.0	36.0	36.0	32.0	36.0
21	33.993512915020524	36.0	36.0	36.0	32.0	36.0
22	33.95104798082359	36.0	36.0	36.0	32.0	36.0
23	33.93488682831999	36.0	36.0	36.0	32.0	36.0
24	33.907351266424875	36.0	36.0	36.0	32.0	36.0
25	33.374477808603324	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	45.0
5	271.0
6	780.0
7	164.0
8	669.0
9	462.0
10	275.0
11	97.0
12	133.0
13	141.0
14	659.0
15	1110.0
16	1843.0
17	2642.0
18	3502.0
19	4533.0
20	5791.0
21	7386.0
22	9873.0
23	13142.0
24	18092.0
25	25211.0
26	34943.0
27	47153.0
28	65394.0
29	90632.0
30	127501.0
31	187402.0
32	291196.0
33	445592.0
34	970192.0
35	1311842.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.694204353501696	18.022462317707983	12.511363224394481	25.771970104395837
2	16.101683827309436	19.584148570624855	38.52850055033628	25.785667051729426
3	18.984537653319176	23.077755492886492	28.4043139527467	29.533392901047634
4	12.791018459656236	16.07366197751344	35.814884399524146	35.320435163306186
5	14.719704209856127	36.316938266622856	33.80116783663921	15.16218968688181
6	34.06558248951621	35.28875251440455	17.05665677951655	13.589008216562682
7	30.29708443705176	30.210014832665916	20.539995263151575	18.952905467130744
8	27.30107126962327	34.28191044492658	19.28192545290673	19.135092832543414
9	26.644538544603414	15.220347465913406	19.100855606771827	39.03425838271136
10	16.220199591907587	27.12419852636934	31.55273267981777	25.102869201905303
11	35.98855896951024	21.629350572215294	22.821929788167886	19.560160670106583
12	24.385771800430526	23.760783229486993	29.19080642603647	22.662638544046015
13	29.025033664419098	20.333036216747004	25.249378702472036	25.392551416361865
14	22.636683887034895	20.430901259913128	25.537166604947686	31.39524824810429
15	24.288019380261762	28.699916537797897	22.080747087693172	24.931316994247165
16	24.74445178539217	26.45500176428353	24.793409630068087	24.007136820256214
17	23.324435560923998	26.971876361990965	25.7990733617287	23.904614715356338
18	23.70360021077386	26.003884198809637	27.03101110055665	23.26150448985985
19	25.06703038283236	25.432720103439742	25.523248977048286	23.97700053667961
20	25.29384000043653	25.36622231437879	25.55068398967604	23.78925369550864
21	25.40315539966868	24.933921474047676	25.002237175527704	24.660685950755948
22	25.139196835758376	25.635656000048023	25.37232390911318	23.85282325508042
23	23.660670077570558	25.761717764298446	25.95982480811961	24.61778735001139
24	24.277677589739532	25.422076593376314	26.05713537389539	24.243110442988762
25	23.986680962397315	25.688554958081316	25.945932673656298	24.37883140586507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	184.0
1	184.0
2	602.5
3	1021.0
4	1021.0
5	1021.0
6	1964.5
7	2908.0
8	2908.0
9	2908.0
10	3856.5
11	4805.0
12	4805.0
13	4805.0
14	7532.5
15	10260.0
16	10260.0
17	10260.0
18	18150.0
19	26040.0
20	26040.0
21	26040.0
22	42199.0
23	58358.0
24	58358.0
25	58358.0
26	87430.0
27	116502.0
28	116502.0
29	116502.0
30	151075.0
31	185648.0
32	185648.0
33	185648.0
34	224765.5
35	263883.0
36	263883.0
37	263883.0
38	300962.5
39	338042.0
40	338042.0
41	338042.0
42	375304.5
43	412567.0
44	412567.0
45	412567.0
46	444174.5
47	475782.0
48	475782.0
49	475782.0
50	479548.5
51	483315.0
52	483315.0
53	483315.0
54	447020.0
55	410725.0
56	410725.0
57	410725.0
58	370909.0
59	331093.0
60	331093.0
61	331093.0
62	284532.5
63	237972.0
64	237972.0
65	237972.0
66	194269.5
67	150567.0
68	150567.0
69	150567.0
70	114868.5
71	79170.0
72	79170.0
73	79170.0
74	59912.5
75	40655.0
76	40655.0
77	40655.0
78	31865.0
79	23075.0
80	23075.0
81	23075.0
82	16183.5
83	9292.0
84	9292.0
85	9292.0
86	6610.0
87	3928.0
88	3928.0
89	3928.0
90	2587.0
91	1246.0
92	1246.0
93	1246.0
94	781.0
95	316.0
96	316.0
97	316.0
98	818.0
99	1320.0
100	1320.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011175699994057798
2	2.1806243890844484E-4
3	5.451560972711122E-4
4	0.009540231702244462
5	0.03295468608003873
6	0.06266569338131434
7	0.10330708043287576
8	0.10785913384508954
9	0.11603647530415621
10	0.1307829477353398
11	0.13677966480532203
12	0.12993795578456957
13	0.12451365261672201
14	0.11489164749988688
15	0.12920199505325355
16	0.11712678749869845
17	0.12159706749632156
18	0.11129361725789753
19	0.09662891824130462
20	0.09305814580417884
21	0.0909865526345486
22	0.10139903409242686
23	0.08120100068853214
24	0.09115009946372994
25	0.08842431897737439
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3668674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.40872864136501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.35475761402115	27.716121810752064
2	16.38380021487615	13.896322748540404
3	7.022787969979497	8.934825279741093
4	3.6682524536430026	6.222636915782698
5	2.1250819304128825	4.506101146377403
6	1.3151866804711492	3.3465237026903156
7	0.8649368741640087	2.567661123183234
8	0.5992062888076441	2.0329261521794217
9	0.4608332977816847	1.758901884707594
>10	2.0013423360224882	14.641634355626262
>50	0.1207126846325657	3.570729117332742
>100	0.07151960698188878	6.0117818828035565
>500	0.007699797425258899	2.171564138420632
>1k	0.003882250780656692	2.622269741862687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3922	0.10690511067486508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0903121945422242E-4
2	0.0	0.0	0.0	0.0	1.0903121945422242E-4
3	0.0	0.0	0.0	0.0	1.0903121945422242E-4
4	0.0	0.0	0.0	0.0	1.0903121945422242E-4
5	0.0	0.0	0.0	0.0	1.0903121945422242E-4
6	0.0	0.0	0.0	0.0	1.0903121945422242E-4
7	0.0	0.0	0.0	2.7257804863555605E-5	1.0903121945422242E-4
8	0.0	0.0	0.0	2.7257804863555605E-5	1.0903121945422242E-4
9	0.0	0.0	0.0	5.451560972711121E-5	1.0903121945422242E-4
10	0.0	0.0	0.0	5.451560972711121E-5	1.3628902431777804E-4
11	0.0	0.0	0.0	5.451560972711121E-5	1.3628902431777804E-4
12	0.0	0.0	0.0	5.451560972711121E-5	3.543514632262229E-4
13	0.0	0.0	0.0	5.451560972711121E-5	4.088670729533341E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	14.662703	1
GTATCAA	3580	0.0	12.573669	1
TCGCGTA	270	0.0	12.316248	9
GCGTAAC	310	0.0	11.953167	11
ATACCGT	510	0.0	11.550048	6
TAATACG	75	2.0754061E-4	11.399925	4
CGCGTAA	320	0.0	11.282717	10
CGTCGTA	485	0.0	11.1664	10
TCGGCGT	175	2.0008883E-11	10.857517	13
CGAACGA	140	8.361894E-9	10.857368	16
CCGTCGT	475	0.0	10.801257	9
TTAGGAC	1285	0.0	10.792378	3
AACCGCG	125	2.2398126E-7	10.641092	7
TGTAGGA	2585	0.0	10.619227	2
TGGCGAG	2060	0.0	10.606149	18
TAGGACC	1600	0.0	10.568682	4
CGACGCT	90	9.521555E-5	10.555632	15
TACCGTC	505	0.0	10.535736	7
ACCGTCG	460	0.0	10.53369	8
AGGACGT	1915	0.0	10.517477	5
>>END_MODULE