FastQCFastQC Report
Thu 2 Feb 2017
SRR4061989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668674
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36700.027.463031
GGTATCA15100.026.670011
TAGGACG23650.021.5806144
GTAGGAC29800.020.9656833
TGTAGGA32650.019.4056452
GGACGTG26100.018.9648286
AGGACGT26050.018.916785
TTAGGAC17000.018.6346193
CTGTAGG32650.017.9960461
TCAACGC55200.017.9739884
ATCAACG55700.017.8521373
GTCCTAC26350.017.7888551
TAGGACC23250.017.5993614
TATCAAC57550.017.431642
CAACGCA57250.017.407235
AACGCAG59600.016.7577826
TCCTACA30750.016.5266822
TGGCGAG30500.016.44530718
GTATAGG5900.016.4115521
TATGGCG28700.016.40358516
Produced by FastQC (version 0.11.5)