FastQCFastQC Report
Thu 2 Feb 2017
SRR4061988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613079
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62690.1735085227862441No Hit
TATCAACGCAGAGTACTTTTTTTTT37450.10365120718367907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37650.013.3156961
GGTATCA12550.011.2729471
GACTTAT1201.2863711E-711.0774981
CGTCGTA1951.8189894E-1210.71928610
CCGTCGT1605.4387783E-1010.6888339
CGTTATT803.7829563E-410.6845382
GCGTTAT909.573126E-510.5499981
CGCCGGT1151.0261603E-59.9140067
CGTCTTA1151.0264985E-59.91373115
CTTAGAC2500.09.878223
CGCGTAA800.0045132459.50118410
ACCGTCG2002.6557245E-109.5009228
ATAATAC2950.09.33733
GTGCTAG2950.09.3340651
ATTATAC2750.09.3255923
ATACCGT2054.2382453E-109.2691936
TTACCGC1752.6708221E-89.22908316
TAGGACG6700.09.2164184
TATCAAC54150.09.20797352
TAATACT4650.09.1935484
Produced by FastQC (version 0.11.5)