##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061988_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3613079
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222558100722402	32.0	32.0	32.0	32.0	32.0
2	30.72053475719739	32.0	32.0	32.0	32.0	32.0
3	30.74700746925268	32.0	32.0	32.0	32.0	32.0
4	30.75450910428474	32.0	32.0	32.0	32.0	32.0
5	30.66506793790006	32.0	32.0	32.0	32.0	32.0
6	34.329215884845034	36.0	36.0	36.0	32.0	36.0
7	34.26290512883887	36.0	36.0	36.0	32.0	36.0
8	34.2177098812398	36.0	36.0	36.0	32.0	36.0
9	34.35015564287413	36.0	36.0	36.0	32.0	36.0
10	34.02081604083387	36.0	36.0	36.0	32.0	36.0
11	34.34764670243856	36.0	36.0	36.0	32.0	36.0
12	34.161401397533794	36.0	36.0	36.0	32.0	36.0
13	34.24251171922894	36.0	36.0	36.0	32.0	36.0
14	34.14339791629245	36.0	36.0	36.0	32.0	36.0
15	34.09607678105018	36.0	36.0	36.0	32.0	36.0
16	34.1025889552927	36.0	36.0	36.0	32.0	36.0
17	34.044270828288006	36.0	36.0	36.0	32.0	36.0
18	34.04556667595699	36.0	36.0	36.0	32.0	36.0
19	34.042687691024746	36.0	36.0	36.0	32.0	36.0
20	34.026846908135695	36.0	36.0	36.0	32.0	36.0
21	34.01548872858855	36.0	36.0	36.0	32.0	36.0
22	33.981666883010305	36.0	36.0	36.0	32.0	36.0
23	33.94979877273649	36.0	36.0	36.0	32.0	36.0
24	33.920781416625545	36.0	36.0	36.0	32.0	36.0
25	33.561036999190996	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	50.0
5	270.0
6	742.0
7	162.0
8	654.0
9	489.0
10	276.0
11	75.0
12	139.0
13	156.0
14	481.0
15	745.0
16	1258.0
17	1755.0
18	2611.0
19	3446.0
20	5166.0
21	7475.0
22	11283.0
23	16649.0
24	23519.0
25	32491.0
26	44887.0
27	57146.0
28	75572.0
29	99513.0
30	129985.0
31	174676.0
32	245881.0
33	345534.0
34	744363.0
35	1585625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.038031664176614	17.199910767713188	11.855799074574833	25.90625849353537
2	16.35282913224187	19.884203706046026	37.719124655521384	26.043842506190725
3	19.02582636507861	24.061153838915132	28.153732633795475	28.759287162210782
4	13.232027866108046	16.099049354721558	35.35227353251194	35.31664924665846
5	14.96766509241986	36.3087317816786	33.55255398426196	15.171049141639575
6	33.78038182183752	34.75897544972103	17.457074538176368	14.003568190265078
7	29.954945781718813	30.163717403850764	21.061191558145598	18.820145256284828
8	27.716677985205823	33.285177590234525	19.572621936132407	19.425522488427244
9	27.186121658832423	15.180433232623876	18.59817204441118	39.03527306413251
10	16.071689383695563	27.237376300255555	31.752162346500352	24.93877196954853
11	35.8023042077512	21.578776987190714	23.108845656661515	19.510073148396568
12	24.819884885961272	23.36474654735484	29.169447332888428	22.64592123379546
13	29.368844326942796	20.365751594863184	25.13945007011301	25.12595400808101
14	23.403786615695942	19.52631142887148	25.64659454250071	31.423307412931862
15	24.76953720093463	27.478554204312687	22.399905994069755	25.352002600682926
16	25.230188340449306	26.07756518539209	23.957127941156912	24.7351185330017
17	23.934256323852175	26.16755714190702	25.447436096393023	24.450750437847784
18	24.148801196545246	25.897845337438564	26.024360380010624	23.92899308600557
19	25.30169047508891	25.284264610506995	25.44475432993949	23.969290584464606
20	25.59529053258536	25.022369970219543	25.272830528429946	24.10950896876515
21	25.48145833437218	24.775345299773228	25.05683200166659	24.686364364187998
22	25.42355553646951	24.86551032932203	25.004896956129645	24.706037178078812
23	24.632120345204683	25.032657940893284	25.29259188265966	25.042629831242376
24	24.669122576455845	25.0769849598149	25.470497501797883	24.783394961931364
25	24.827738469405677	25.028158534348982	25.36329080051126	24.780812195734086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	497.0
1	497.0
2	904.0
3	1311.0
4	1311.0
5	1311.0
6	2825.0
7	4339.0
8	4339.0
9	4339.0
10	4877.5
11	5416.0
12	5416.0
13	5416.0
14	7610.5
15	9805.0
16	9805.0
17	9805.0
18	16650.0
19	23495.0
20	23495.0
21	23495.0
22	36762.5
23	50030.0
24	50030.0
25	50030.0
26	74338.5
27	98647.0
28	98647.0
29	98647.0
30	129061.0
31	159475.0
32	159475.0
33	159475.0
34	195980.0
35	232485.0
36	232485.0
37	232485.0
38	273360.0
39	314235.0
40	314235.0
41	314235.0
42	357001.0
43	399767.0
44	399767.0
45	399767.0
46	434651.0
47	469535.0
48	469535.0
49	469535.0
50	483094.5
51	496654.0
52	496654.0
53	496654.0
54	471120.5
55	445587.0
56	445587.0
57	445587.0
58	400998.0
59	356409.0
60	356409.0
61	356409.0
62	303920.5
63	251432.0
64	251432.0
65	251432.0
66	202837.0
67	154242.0
68	154242.0
69	154242.0
70	115719.5
71	77197.0
72	77197.0
73	77197.0
74	56024.5
75	34852.0
76	34852.0
77	34852.0
78	25631.0
79	16410.0
80	16410.0
81	16410.0
82	11231.0
83	6052.0
84	6052.0
85	6052.0
86	4319.0
87	2586.0
88	2586.0
89	2586.0
90	1715.0
91	844.0
92	844.0
93	844.0
94	594.0
95	344.0
96	344.0
97	344.0
98	888.5
99	1433.0
100	1433.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00102405732063982
2	2.7677224882157294E-4
3	7.47285071818247E-4
4	0.010378959330808986
5	0.033018929284413655
6	0.06324245885572942
7	0.10716621474371306
8	0.10949110163381427
9	0.12034057378761992
10	0.13412383177893425
11	0.14035120737741963
12	0.1337086734057019
13	0.12784110173068455
14	0.11652111675388221
15	0.1327399705348264
16	0.11887368086886559
17	0.1250733792424688
18	0.11131779847603665
19	0.09692564153731485
20	0.09213748163270163
21	0.09180535493411575
22	0.10223966871468905
23	0.08112194612960304
24	0.09086432928812241
25	0.08748770785249921
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3613079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.25669464610055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.39789512318217	33.00656339395412
2	15.931094723185426	15.375639468697258
3	6.23978481579735	9.033341715399224
4	3.0546977257702763	5.896384615545362
5	1.7423239313289867	4.2039396964368185
6	1.0516648892701772	3.044992285892165
7	0.7129698617208613	2.4083898236255297
8	0.4910958635227852	1.8958930502385691
9	0.3651612029640617	1.5859325401235524
>10	1.8713011915438615	15.887748214514383
>50	0.0992367764854211	3.2767860549795365
>100	0.04104137170206174	3.3980629599671888
>500	0.0010972649050333067	0.3499741256844262
>1k	5.775078377250794E-4	0.4616436800434313
>5k	5.775078377250789E-5	0.17470837489841648
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6269	0.1735085227862441	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3745	0.10365120718367907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8748114835020214E-4
2	0.0	0.0	0.0	0.0	3.8748114835020214E-4
3	0.0	0.0	0.0	0.0	3.8748114835020214E-4
4	0.0	0.0	0.0	0.0	3.8748114835020214E-4
5	0.0	0.0	0.0	0.0	4.428355981145167E-4
6	0.0	0.0	0.0	0.0	4.981900478788314E-4
7	0.0	0.0	0.0	0.0	4.981900478788314E-4
8	0.0	0.0	0.0	0.0	4.981900478788314E-4
9	0.0	0.0	0.0	0.0	4.981900478788314E-4
10	0.0	0.0	0.0	0.0	5.812217225253032E-4
11	0.0	0.0	0.0	2.7677224882157295E-5	5.812217225253032E-4
12	0.0	0.0	0.0	2.7677224882157295E-5	9.410256459933481E-4
13	0.0	0.0	0.0	2.7677224882157295E-5	9.963800957576627E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3765	0.0	13.315696	1
GGTATCA	1255	0.0	11.272947	1
GACTTAT	120	1.2863711E-7	11.077498	1
CGTCGTA	195	1.8189894E-12	10.719286	10
CCGTCGT	160	5.4387783E-10	10.688833	9
CGTTATT	80	3.7829563E-4	10.684538	2
GCGTTAT	90	9.573126E-5	10.549998	1
CGCCGGT	115	1.0261603E-5	9.914006	7
CGTCTTA	115	1.0264985E-5	9.913731	15
CTTAGAC	250	0.0	9.87822	3
CGCGTAA	80	0.004513245	9.501184	10
ACCGTCG	200	2.6557245E-10	9.500922	8
ATAATAC	295	0.0	9.3373	3
GTGCTAG	295	0.0	9.334065	1
ATTATAC	275	0.0	9.325592	3
ATACCGT	205	4.2382453E-10	9.269193	6
TTACCGC	175	2.6708221E-8	9.229083	16
TAGGACG	670	0.0	9.216418	4
TATCAAC	5415	0.0	9.2079735	2
TAATACT	465	0.0	9.193548	4
>>END_MODULE