FastQCFastQC Report
Thu 2 Feb 2017
SRR4061988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613079
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41500.026.6738011
GGTATCA17450.020.1785341
TATCAAC60000.018.5529942
TAGGACG9050.018.231464
TCAACGC60750.018.0702024
ATCAACG62400.017.627643
AACGCAG63650.017.4194686
CAACGCA63050.017.4107785
GTAGGAC12650.017.0429333
TTAGGAC7900.016.4298523
TAGGACC10750.015.9622784
TGTAGGA15100.015.589122
TAGCGTA1756.424125E-815.0850217
ACGCAGA73500.015.025167
GCGTAAA900.00435174814.6714751
CGTATAC1057.1654405E-414.6661963
GTATAAA6700.014.4524981
GTCCTAC12300.014.3136341
CGCAGAG78450.014.2453628
GTACATG101100.014.0837461
Produced by FastQC (version 0.11.5)