##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3613079
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286229833336055	32.0	32.0	32.0	32.0	32.0
2	31.446194506126215	32.0	32.0	32.0	32.0	32.0
3	31.56880793362116	32.0	32.0	32.0	32.0	32.0
4	31.653228174639967	32.0	32.0	32.0	32.0	32.0
5	31.628614818552265	32.0	32.0	32.0	32.0	32.0
6	35.27108762360303	36.0	36.0	36.0	36.0	36.0
7	35.280029027873454	36.0	36.0	36.0	36.0	36.0
8	35.233208573629305	36.0	36.0	36.0	36.0	36.0
9	35.31437175882398	36.0	36.0	36.0	36.0	36.0
10	35.196368803449914	36.0	36.0	36.0	36.0	36.0
11	35.32005416986454	36.0	36.0	36.0	36.0	36.0
12	35.24956166195093	36.0	36.0	36.0	36.0	36.0
13	35.275660454698055	36.0	36.0	36.0	36.0	36.0
14	35.24552300129612	36.0	36.0	36.0	36.0	36.0
15	35.22005248155382	36.0	36.0	36.0	36.0	36.0
16	35.23523703744092	36.0	36.0	36.0	36.0	36.0
17	35.21864260371832	36.0	36.0	36.0	36.0	36.0
18	35.21468503733242	36.0	36.0	36.0	36.0	36.0
19	35.20290616396708	36.0	36.0	36.0	36.0	36.0
20	35.202843613438844	36.0	36.0	36.0	36.0	36.0
21	35.19485458247661	36.0	36.0	36.0	36.0	36.0
22	35.17511906050213	36.0	36.0	36.0	36.0	36.0
23	35.15006286881632	36.0	36.0	36.0	36.0	36.0
24	35.13659347055517	36.0	36.0	36.0	36.0	36.0
25	35.12289628873324	36.0	36.0	36.0	36.0	36.0
26	35.08187310601291	36.0	36.0	36.0	36.0	36.0
27	35.06563930653052	36.0	36.0	36.0	36.0	36.0
28	35.044510513055485	36.0	36.0	36.0	36.0	36.0
29	35.02967690437989	36.0	36.0	36.0	36.0	36.0
30	35.00723676398994	36.0	36.0	36.0	36.0	36.0
31	35.007932569423474	36.0	36.0	36.0	36.0	36.0
32	34.984355171863115	36.0	36.0	36.0	36.0	36.0
33	34.965224397252314	36.0	36.0	36.0	36.0	36.0
34	34.95714237081448	36.0	36.0	36.0	36.0	36.0
35	34.94344408190355	36.0	36.0	36.0	36.0	36.0
36	34.91823760288663	36.0	36.0	36.0	36.0	36.0
37	34.90028836900605	36.0	36.0	36.0	36.0	36.0
38	34.89019503863602	36.0	36.0	36.0	36.0	36.0
39	34.862092691579676	36.0	36.0	36.0	32.0	36.0
40	34.85658132578889	36.0	36.0	36.0	32.0	36.0
41	34.82902422006272	36.0	36.0	36.0	32.0	36.0
42	34.811833065371665	36.0	36.0	36.0	32.0	36.0
43	34.80374328931086	36.0	36.0	36.0	32.0	36.0
44	34.76834052064735	36.0	36.0	36.0	32.0	36.0
45	34.749105679671	36.0	36.0	36.0	32.0	36.0
46	34.726871457834164	36.0	36.0	36.0	32.0	36.0
47	34.694974563246475	36.0	36.0	36.0	32.0	36.0
48	34.67662760764434	36.0	36.0	36.0	32.0	36.0
49	34.6579643013618	36.0	36.0	36.0	32.0	36.0
50	34.25849088824241	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	8.0
20	38.0
21	91.0
22	245.0
23	629.0
24	1649.0
25	3942.0
26	8331.0
27	15669.0
28	26848.0
29	43900.0
30	65554.0
31	96722.0
32	146780.0
33	248756.0
34	572669.0
35	2381242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.685520378903384	17.706844907260646	12.250598508207737	26.35703620562823
2	15.839156668023838	20.27530739097353	37.424212690777814	26.461323250224815
3	18.422238385770765	24.52231732145645	28.337800451029434	28.717643841743364
4	12.95064761956426	16.504155182921597	35.5087545854255	35.03644261208864
5	14.70117722340896	36.62118559300408	33.36435028526923	15.31328689831772
6	33.94424416874823	34.901102246701214	17.322170173605585	13.83248341094497
7	29.99367021866945	30.176699706815157	21.08265554116032	18.74697453335507
8	27.79645836694963	33.299216540795264	19.489637508617996	19.414687583637114
9	27.263920882992043	14.934713578086722	18.514015331521954	39.287350207399285
10	16.012016360583814	27.220948145540337	31.840615498049583	24.92641999582627
11	36.00734996383971	21.469721531137292	23.064289488273022	19.458639016749977
12	24.87388180551823	23.270733908669033	29.16307669995591	22.692307585856828
13	29.451833187151454	20.239385853450756	25.194660841902433	25.114120117495354
14	23.440118524947838	19.322190298080944	25.684270950067795	31.553420226903423
15	24.885063404370623	27.369730913716527	22.40969544258512	25.33551023932773
16	25.33709783933003	25.992721930470854	23.988568094621545	24.68161213557757
17	24.042679387857284	26.08434523573938	25.390587916843227	24.482387459560115
18	24.30921626207076	25.74918282789982	26.037026683678977	23.904574226350444
19	25.40758173292087	25.17498233501122	25.496370270342826	23.921065661725084
20	25.691517569002297	24.893414682480376	25.299136357245423	24.115931391271907
21	25.579167009172515	24.66451725219252	25.053354799800836	24.702960938834128
22	25.503898475510777	24.71786528885751	25.09875925768576	24.679476977945956
23	24.713727385272996	24.880510434734717	25.384263883664808	25.021498296327476
24	24.87230335547065	24.9338579124868	25.398506250250826	24.795332481791725
25	24.983504323749234	24.754557622497003	25.50231548368312	24.759622570070643
26	24.68894770025834	25.10992073750269	25.518300564231183	24.68283099800778
27	24.758501238008094	25.400117684113916	25.103139663808705	24.738241414069286
28	24.7577726291305	25.053756804190165	25.182762063209534	25.005708503469805
29	24.888923946842915	25.09738961242048	25.109761379881068	24.903925060855535
30	24.852166976794262	25.13414644609827	25.203285110637086	24.810401466470378
31	25.22808172221455	24.678576578526453	25.179258962394574	24.914082736864422
32	24.87423347183151	25.141791796612495	25.08007098723403	24.903903744321962
33	24.70365221367209	25.113663757874537	25.25025124070423	24.93243278774914
34	24.97972627004885	25.036354326676907	25.384093771190546	24.599825632083697
35	24.95335682767199	25.134200315576592	25.325865628361928	24.586577228389494
36	24.704129892789297	25.254105093947754	25.29005787872481	24.751707134538144
37	25.164315948285765	24.87505566689704	25.027089608689046	24.933538776128152
38	24.975457220835747	25.150155864291925	25.1115738128062	24.762813102066133
39	25.16140085368223	25.053458929758595	24.903890171583377	24.881250044975804
40	25.23015028200363	24.964171782808766	25.228545000738983	24.577132934448617
41	24.966212691075086	24.954172978290046	25.321259669480824	24.758354661154048
42	25.01469039457168	25.20821887096395	25.083526967910863	24.69356376655351
43	24.82607572871052	24.992284640442104	25.25785907975343	24.923780551093948
44	24.75068164500432	25.1332221223039	25.107315322017932	25.00878091067385
45	24.770813093864085	25.311251899396346	25.08742066820002	24.830514338539547
46	25.02217696568182	24.95342491232314	25.045094316801382	24.97930380519366
47	25.08995889609827	25.119020303960184	25.061284973674514	24.72973582626703
48	24.927642211390197	25.464018451409082	24.802730401121078	24.80560893607964
49	25.01580428592306	25.111432670974143	24.885578077047416	24.987184966055384
50	24.82302952201918	25.52864187197899	24.861169234039444	24.787159371962385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	151.0
1	140.0
2	129.0
3	370.0
4	611.0
5	617.5
6	624.0
7	527.0
8	430.0
9	588.5
10	747.0
11	1095.5
12	1444.0
13	2619.0
14	3794.0
15	4864.0
16	5934.0
17	5808.5
18	5683.0
19	5708.5
20	5734.0
21	6876.0
22	8018.0
23	10000.5
24	11983.0
25	15438.5
26	18894.0
27	24760.5
28	30627.0
29	37831.0
30	45035.0
31	54004.5
32	62974.0
33	74858.0
34	86742.0
35	99978.5
36	113215.0
37	126139.0
38	139063.0
39	152180.0
40	165297.0
41	178606.0
42	191915.0
43	202060.0
44	212205.0
45	226342.0
46	240479.0
47	252002.5
48	263526.0
49	269723.0
50	275920.0
51	278867.5
52	281815.0
53	280274.5
54	278734.0
55	269489.5
56	260245.0
57	246852.5
58	233460.0
59	215208.5
60	196957.0
61	174786.5
62	152616.0
63	131153.0
64	109690.0
65	91826.0
66	73962.0
67	61959.0
68	49956.0
69	40934.0
70	31912.0
71	25980.5
72	20049.0
73	16582.0
74	13115.0
75	10533.0
76	7951.0
77	6483.5
78	5016.0
79	4074.0
80	3132.0
81	2397.0
82	1662.0
83	1276.5
84	891.0
85	647.0
86	403.0
87	271.0
88	139.0
89	105.0
90	71.0
91	50.5
92	30.0
93	30.5
94	31.0
95	33.0
96	35.0
97	32.5
98	30.0
99	16.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03271447981070993
2	0.004760482679731054
3	3.0444947370373025E-4
4	2.7677224882157295E-5
5	2.7677224882157295E-5
6	1.1070889952862918E-4
7	0.0
8	0.0
9	0.0
10	1.3838612441078647E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.133484211111908E-4
17	0.0
18	2.490950239394157E-4
19	0.0
20	3.0444947370373025E-4
21	5.535444976431459E-5
22	0.0
23	5.535444976431459E-5
24	6.642533971717751E-4
25	3.0444947370373025E-4
26	5.812217225253032E-4
27	4.7051282299667404E-4
28	0.0013285067943435503
29	3.874811483502021E-4
30	0.0013838612441078647
31	2.7677224882157294E-4
32	9.687028708755053E-4
33	2.7677224882157294E-4
34	8.026395215825616E-4
35	2.7677224882157294E-4
36	1.9374057417510104E-4
37	0.0019374057417510106
38	0.0
39	6.919306220539323E-4
40	1.3838612441078647E-4
41	9.963800957576627E-4
42	0.004815837129495369
43	0.004317647081616538
44	0.0035426847849161338
45	0.0024632730145119994
46	0.0026570135886871005
47	0.0011070889952862917
48	0.0035980392346804486
49	0.0037364253590912353
50	0.0013008295694613928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3613079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.97407788771141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.38180116750134	32.99961578591494
2	16.590002484736278	16.249601476896004
3	6.488426885759687	9.532941710157472
4	3.2147755784097827	6.297626782742127
5	1.7654160912315402	4.322981257809624
6	1.0843869521540306	3.186411063312569
7	0.6993487467728381	2.3974971996588437
8	0.4928995378916544	1.9311440287618276
9	0.3660968261054223	1.61363290285178
>10	1.7898184849608596	15.516051253818802
>50	0.0938452833491232	3.1110580051350216
>100	0.032556860817329884	2.6064643863034855
>500	5.682730112202092E-4	0.18128883126578396
>1k	5.6827298859339074E-5	0.05368531537176578
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3838612441078647E-4
2	0.0	0.0	0.0	0.0	1.3838612441078647E-4
3	0.0	0.0	0.0	0.0	1.3838612441078647E-4
4	0.0	0.0	0.0	0.0	1.3838612441078647E-4
5	0.0	0.0	0.0	0.0	1.3838612441078647E-4
6	0.0	0.0	0.0	0.0	2.490950239394157E-4
7	0.0	0.0	0.0	0.0	2.490950239394157E-4
8	0.0	0.0	0.0	0.0	2.490950239394157E-4
9	0.0	0.0	0.0	0.0	2.490950239394157E-4
10	0.0	0.0	0.0	0.0	2.490950239394157E-4
11	0.0	0.0	0.0	2.7677224882157295E-5	2.7677224882157294E-4
12	0.0	0.0	0.0	2.7677224882157295E-5	6.642533971717751E-4
13	0.0	0.0	0.0	2.7677224882157295E-5	7.196078469360897E-4
14	0.0	0.0	0.0	5.535444976431459E-5	7.196078469360897E-4
15	0.0	0.0	0.0	1.1070889952862918E-4	8.026395215825616E-4
16	0.0	0.0	0.0	1.6606334929294378E-4	9.410256459933481E-4
17	0.0	0.0	0.0	2.2141779905725836E-4	9.687028708755054E-4
18	0.0	0.0	0.0	3.3212669858588757E-4	0.00102405732063982
19	0.0	0.0	0.0	3.5980392346804483E-4	0.0010517345455219773
20	0.0	0.0	0.0	4.981900478788314E-4	0.0012731523445792356
21	0.0	0.0	0.0	6.365761722896178E-4	0.001411538468990022
22	2.7677224882157295E-5	0.0	0.0	8.303167464647189E-4	0.001411538468990022
23	2.7677224882157295E-5	0.0	0.0	0.0014668929187543367	0.001411538468990022
24	2.7677224882157295E-5	0.0	0.0	0.001965082966633168	0.001411538468990022
25	2.7677224882157295E-5	0.0	0.0	0.0023802413398655276	0.001411538468990022
26	2.7677224882157295E-5	0.0	0.0	0.0029337858375086735	0.0016329562680472804
27	2.7677224882157295E-5	0.0	0.0	0.003847134258619864	0.001688310717811595
28	2.7677224882157295E-5	0.0	0.0	0.0046497737802024255	0.001688310717811595
29	2.7677224882157295E-5	0.0	0.0	0.0053970588520206725	0.001688310717811595
30	2.7677224882157295E-5	0.0	0.0	0.006697888421482065	0.001688310717811595
31	2.7677224882157295E-5	0.0	0.0	0.009299547560404851	0.0017159879426937523
32	2.7677224882157295E-5	0.0	0.0	0.012620814546263726	0.0017713423924580669
33	2.7677224882157295E-5	0.0	0.0	0.015499245934008086	0.0017713423924580669
34	2.7677224882157295E-5	0.0	0.0	0.019097285168688534	0.0018543740671045387
35	2.7677224882157295E-5	0.0	0.0	0.0233319005756586	0.0018543740671045387
36	2.7677224882157295E-5	0.0	0.0	0.02701297148498552	0.0018543740671045387
37	2.7677224882157295E-5	0.0	0.0	0.03481794890175388	0.0018543740671045387
38	2.7677224882157295E-5	0.0	0.0	0.04489245875885913	0.0019374057417510108
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4150	0.0	26.673801	1
GGTATCA	1745	0.0	20.178534	1
TATCAAC	6000	0.0	18.552994	2
TAGGACG	905	0.0	18.23146	4
TCAACGC	6075	0.0	18.070202	4
ATCAACG	6240	0.0	17.62764	3
AACGCAG	6365	0.0	17.419468	6
CAACGCA	6305	0.0	17.410778	5
GTAGGAC	1265	0.0	17.042933	3
TTAGGAC	790	0.0	16.429852	3
TAGGACC	1075	0.0	15.962278	4
TGTAGGA	1510	0.0	15.58912	2
TAGCGTA	175	6.424125E-8	15.085021	7
ACGCAGA	7350	0.0	15.02516	7
GCGTAAA	90	0.004351748	14.671475	1
CGTATAC	105	7.1654405E-4	14.666196	3
GTATAAA	670	0.0	14.452498	1
GTCCTAC	1230	0.0	14.313634	1
CGCAGAG	7845	0.0	14.245362	8
GTACATG	10110	0.0	14.083746	1
>>END_MODULE