FastQCFastQC Report
Thu 2 Feb 2017
SRR4061987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505164
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25450.16908456487133627No Hit
TATCAACGCAGAGTACTTTTTTTTT15800.10497194990047595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAA653.3837296E-614.6104731
GGTATCA6650.014.4237221
AACCGCG500.001497667513.3021677
GTATCAA20950.012.6926311
TAGGAGT1051.9905201E-812.6678864
GATCTAG751.4836258E-512.6624091
TCGCCAG2050.012.5100717
CGAGCCG1850.012.323050515
CGTCGTA1650.012.09287810
GTATTAC550.003076100712.0868451
CGGTCCA1750.011.94480210
GATCGAA802.8624147E-511.87653911
ATCGCCA2250.011.82060716
GGACGAT658.012196E-411.6942136
CGACCAT1800.011.61300310
CGCCAGT2150.011.48680318
CGCATCG2250.011.3999613
AAATCGC1001.9308736E-611.39920114
AGCGCCC752.0770029E-411.39806419
GGTTCCG1354.7166395E-911.2607188
Produced by FastQC (version 0.11.5)