##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061987_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505164
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21594656794874	32.0	32.0	32.0	32.0	32.0
2	30.72820237528934	32.0	32.0	32.0	32.0	32.0
3	30.750725502337286	32.0	32.0	32.0	32.0	32.0
4	30.758573152161492	32.0	32.0	32.0	32.0	32.0
5	30.677476341448507	32.0	32.0	32.0	32.0	32.0
6	34.33250064444805	36.0	36.0	36.0	32.0	36.0
7	34.261293121546885	36.0	36.0	36.0	32.0	36.0
8	34.20922703439625	36.0	36.0	36.0	32.0	36.0
9	34.33979420182784	36.0	36.0	36.0	32.0	36.0
10	34.026794422401814	36.0	36.0	36.0	32.0	36.0
11	34.35093385172646	36.0	36.0	36.0	32.0	36.0
12	34.15350088096712	36.0	36.0	36.0	32.0	36.0
13	34.23822719650483	36.0	36.0	36.0	32.0	36.0
14	34.13333696527422	36.0	36.0	36.0	32.0	36.0
15	34.08804887706589	36.0	36.0	36.0	32.0	36.0
16	34.091766079975336	36.0	36.0	36.0	32.0	36.0
17	34.03513238424517	36.0	36.0	36.0	32.0	36.0
18	34.036528909806506	36.0	36.0	36.0	32.0	36.0
19	34.04493131645455	36.0	36.0	36.0	32.0	36.0
20	34.02216104025874	36.0	36.0	36.0	32.0	36.0
21	34.00313188463184	36.0	36.0	36.0	32.0	36.0
22	33.97075335312298	36.0	36.0	36.0	32.0	36.0
23	33.93107860671661	36.0	36.0	36.0	32.0	36.0
24	33.9059345028183	36.0	36.0	36.0	32.0	36.0
25	33.55525178651629	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	25.0
5	132.0
6	330.0
7	77.0
8	301.0
9	212.0
10	122.0
11	21.0
12	58.0
13	66.0
14	252.0
15	379.0
16	601.0
17	820.0
18	1222.0
19	1667.0
20	2438.0
21	3501.0
22	4956.0
23	7242.0
24	9761.0
25	13840.0
26	18847.0
27	23835.0
28	31297.0
29	41093.0
30	52994.0
31	71519.0
32	100510.0
33	140621.0
34	305686.0
35	670738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.008288238010294	17.726597769650102	11.903105680848025	25.362008311491586
2	16.31716824224981	19.60373569003101	37.944745963377905	26.134350104341276
3	19.364037229487273	23.22484587925065	28.390241244898352	29.02087564636372
4	12.710125003571434	15.70282199898073	35.5706356889748	36.016417308473045
5	14.782049262642152	36.5396386750488	33.43721276141826	15.241099300890784
6	33.91189706720243	35.698315368316635	16.776043512283202	13.61374405219772
7	30.339166692717356	30.465806306572958	19.991499714994358	19.20352728571533
8	28.397584407066915	33.19298469667733	18.992230272868348	19.417200623387405
9	27.091697072705077	14.533232663568965	18.62013683548809	39.75493342823787
10	15.98277656100722	27.05393235701911	31.29931579941826	25.663975282555413
11	36.86088599677964	21.720427961196055	21.702995462227367	19.71569057979693
12	24.16609893443757	24.13163581396834	28.896794264485155	22.80547098710893
13	29.244970461440207	20.255269040395977	24.976981212411516	25.5227792857523
14	23.255300368047394	20.006399088961306	25.210547986847942	31.52775255614336
15	25.455765983577656	27.177637295302826	22.03224857857963	25.334348142539888
16	25.53940634868051	26.56436987825897	23.524403085800117	24.3718206872604
17	23.598286891178475	26.320022458276565	25.20223092643052	24.87945972411444
18	24.074875963902983	26.14931691181215	25.717094598684444	24.058712525600427
19	25.74392084175467	25.356123650840207	25.05019549474236	23.849760012662767
20	25.525036842357274	25.197046759202607	25.097027074477424	24.180889323962695
21	26.203771868397585	24.43501444093978	24.937704120007105	24.423509570655533
22	25.478666517686122	25.08021006257183	25.027667815945176	24.413455603796876
23	24.520864392421075	24.94364479530593	25.262425740796644	25.273065071476353
24	24.76301694360688	25.722919689777306	24.892963594986746	24.62109977162907
25	24.617586308253166	25.096792127351975	25.036542269996502	25.249079294398356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	464.0
1	464.0
2	530.0
3	596.0
4	596.0
5	596.0
6	1122.5
7	1649.0
8	1649.0
9	1649.0
10	2058.0
11	2467.0
12	2467.0
13	2467.0
14	3547.0
15	4627.0
16	4627.0
17	4627.0
18	7387.5
19	10148.0
20	10148.0
21	10148.0
22	15633.5
23	21119.0
24	21119.0
25	21119.0
26	32141.0
27	43163.0
28	43163.0
29	43163.0
30	55992.0
31	68821.0
32	68821.0
33	68821.0
34	82227.5
35	95634.0
36	95634.0
37	95634.0
38	111474.0
39	127314.0
40	127314.0
41	127314.0
42	144077.5
43	160841.0
44	160841.0
45	160841.0
46	179183.0
47	197525.0
48	197525.0
49	197525.0
50	199734.0
51	201943.0
52	201943.0
53	201943.0
54	191241.5
55	180540.0
56	180540.0
57	180540.0
58	161664.5
59	142789.0
60	142789.0
61	142789.0
62	124117.0
63	105445.0
64	105445.0
65	105445.0
66	86297.5
67	67150.0
68	67150.0
69	67150.0
70	51764.5
71	36379.0
72	36379.0
73	36379.0
74	27804.5
75	19230.0
76	19230.0
77	19230.0
78	14834.5
79	10439.0
80	10439.0
81	10439.0
82	7125.5
83	3812.0
84	3812.0
85	3812.0
86	2788.0
87	1764.0
88	1764.0
89	1764.0
90	1138.0
91	512.0
92	512.0
93	512.0
94	342.0
95	172.0
96	172.0
97	172.0
98	396.5
99	621.0
100	621.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012623209165247109
2	4.6506560082489355E-4
3	8.636932586748023E-4
4	0.011095136476822458
5	0.03554429949161686
6	0.06889614686505922
7	0.11194793391284935
8	0.11726296935084815
9	0.12616565370949612
10	0.1392539284755681
11	0.14775798517636618
12	0.14005118379126794
13	0.1357991554408689
14	0.12124924592934723
15	0.1388553008177182
16	0.12563415016569623
17	0.12835810582767063
18	0.11766159700869806
19	0.10251374601040152
20	0.09699939674347778
21	0.09653433114265289
22	0.10709796407567548
23	0.08703370529723006
24	0.09766377617322762
25	0.09474050668232831
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1505164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.44155839327872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90042408344533	36.7721099829989
2	15.092353452556706	15.225636559382819
3	5.318903423562565	8.048813328835237
4	2.3520330349315275	4.745608466976769
5	1.1881021385160617	2.9964861698568623
6	0.6990248101782248	2.11559404685733
7	0.4577717775106423	1.616350529226864
8	0.3009102831897465	1.2142706896522908
9	0.2158436360460037	0.9798740434288886
>10	1.2763286088181591	11.770395057541833
>50	0.11085503042733671	3.8957946894816207
>100	0.08042731175838111	7.96307535303528
>500	0.0063599176386654275	2.1694381113553423
>1k	6.624914206943154E-4	0.48655297136989806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2545	0.16908456487133627	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1580	0.10497194990047595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.643794297498479E-5
6	0.0	0.0	0.0	0.0	6.643794297498479E-5
7	0.0	0.0	0.0	0.0	6.643794297498479E-5
8	0.0	0.0	0.0	0.0	6.643794297498479E-5
9	0.0	0.0	0.0	0.0	6.643794297498479E-5
10	0.0	0.0	0.0	0.0	6.643794297498479E-5
11	0.0	0.0	0.0	0.0	6.643794297498479E-5
12	0.0	0.0	0.0	0.0	5.315035437998783E-4
13	0.0	0.0	0.0	0.0	5.979414867748631E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTAA	65	3.3837296E-6	14.610473	1
GGTATCA	665	0.0	14.423722	1
AACCGCG	50	0.0014976675	13.302167	7
GTATCAA	2095	0.0	12.692631	1
TAGGAGT	105	1.9905201E-8	12.667886	4
GATCTAG	75	1.4836258E-5	12.662409	1
TCGCCAG	205	0.0	12.51007	17
CGAGCCG	185	0.0	12.3230505	15
CGTCGTA	165	0.0	12.092878	10
GTATTAC	55	0.0030761007	12.086845	1
CGGTCCA	175	0.0	11.944802	10
GATCGAA	80	2.8624147E-5	11.876539	11
ATCGCCA	225	0.0	11.820607	16
GGACGAT	65	8.012196E-4	11.694213	6
CGACCAT	180	0.0	11.613003	10
CGCCAGT	215	0.0	11.486803	18
CGCATCG	225	0.0	11.39996	13
AAATCGC	100	1.9308736E-6	11.399201	14
AGCGCCC	75	2.0770029E-4	11.398064	19
GGTTCCG	135	4.7166395E-9	11.260718	8
>>END_MODULE