FastQCFastQC Report
Thu 2 Feb 2017
SRR4061987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505164
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.028.859891
GTATCAA24350.027.292451
CTAGCGG2500.019.35919629
TCAACGC34200.019.233124
ATCAACG34900.018.8473553
AACGCAG35900.018.3836386
TATCAAC38500.017.5432952
CAACGCA37650.017.4707245
TACGAAT2650.017.43613441
GTATTAA3300.017.3377971
CGCAATA2700.017.11210636
GATATAC2850.016.986831
TAGGACG2350.016.8503654
TTAGGAC2900.016.6889613
ATACGAA2650.016.60584340
TAGCGGC3050.016.58947630
TAGGACA3200.016.4993154
GCGCTAT1206.4111628E-616.49931511
TCTAGCG2800.016.49931328
TACTAGA1351.0855947E-616.2967032
Produced by FastQC (version 0.11.5)