##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505164
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286154199808127	32.0	32.0	32.0	32.0	32.0
2	31.462699081296126	32.0	32.0	32.0	32.0	32.0
3	31.584126380912643	32.0	32.0	32.0	32.0	32.0
4	31.658920223975592	32.0	32.0	32.0	32.0	32.0
5	31.639685110725477	32.0	32.0	32.0	32.0	32.0
6	35.282488154114766	36.0	36.0	36.0	36.0	36.0
7	35.28698334533645	36.0	36.0	36.0	36.0	36.0
8	35.24136838244869	36.0	36.0	36.0	36.0	36.0
9	35.317400628768695	36.0	36.0	36.0	36.0	36.0
10	35.208102904401116	36.0	36.0	36.0	36.0	36.0
11	35.32903324820418	36.0	36.0	36.0	36.0	36.0
12	35.25411184429072	36.0	36.0	36.0	36.0	36.0
13	35.28019338756441	36.0	36.0	36.0	36.0	36.0
14	35.24156570313932	36.0	36.0	36.0	36.0	36.0
15	35.22679189775998	36.0	36.0	36.0	36.0	36.0
16	35.23535508423002	36.0	36.0	36.0	36.0	36.0
17	35.218432011395436	36.0	36.0	36.0	36.0	36.0
18	35.21444839233465	36.0	36.0	36.0	36.0	36.0
19	35.20871546223535	36.0	36.0	36.0	36.0	36.0
20	35.20718672516749	36.0	36.0	36.0	36.0	36.0
21	35.19592416507437	36.0	36.0	36.0	36.0	36.0
22	35.17502876762931	36.0	36.0	36.0	36.0	36.0
23	35.14939966674728	36.0	36.0	36.0	36.0	36.0
24	35.13419600787688	36.0	36.0	36.0	36.0	36.0
25	35.11336173333936	36.0	36.0	36.0	36.0	36.0
26	35.07655179103406	36.0	36.0	36.0	36.0	36.0
27	35.06261909001278	36.0	36.0	36.0	36.0	36.0
28	35.039689362753826	36.0	36.0	36.0	36.0	36.0
29	35.02064426202062	36.0	36.0	36.0	36.0	36.0
30	35.00347869069417	36.0	36.0	36.0	36.0	36.0
31	34.99633196116835	36.0	36.0	36.0	36.0	36.0
32	34.971672854253754	36.0	36.0	36.0	36.0	36.0
33	34.94384665059754	36.0	36.0	36.0	36.0	36.0
34	34.94273979446758	36.0	36.0	36.0	36.0	36.0
35	34.916797106494705	36.0	36.0	36.0	36.0	36.0
36	34.89989396504301	36.0	36.0	36.0	32.0	36.0
37	34.87934271614256	36.0	36.0	36.0	32.0	36.0
38	34.87434326093369	36.0	36.0	36.0	32.0	36.0
39	34.85528487261189	36.0	36.0	36.0	32.0	36.0
40	34.84609650509845	36.0	36.0	36.0	32.0	36.0
41	34.811096996739224	36.0	36.0	36.0	32.0	36.0
42	34.78232205925733	36.0	36.0	36.0	32.0	36.0
43	34.778558349787794	36.0	36.0	36.0	32.0	36.0
44	34.741437477909386	36.0	36.0	36.0	32.0	36.0
45	34.714987204052186	36.0	36.0	36.0	32.0	36.0
46	34.696003890605944	36.0	36.0	36.0	32.0	36.0
47	34.66989909405221	36.0	36.0	36.0	32.0	36.0
48	34.64611696798488	36.0	36.0	36.0	32.0	36.0
49	34.627707013986516	36.0	36.0	36.0	32.0	36.0
50	34.22673608988788	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	19.0
21	33.0
22	107.0
23	284.0
24	692.0
25	1631.0
26	3628.0
27	6826.0
28	11564.0
29	18451.0
30	27559.0
31	40598.0
32	61459.0
33	103433.0
34	237771.0
35	991100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91566144950653	18.180972319143986	12.263449971754229	25.639916259595253
2	15.841762429705856	20.08439283929881	37.69759431906367	26.376250411931668
3	18.715560002498083	23.67192991547709	28.631023802215587	28.981486279809243
4	12.494585307647538	16.062236407461246	35.696973884573374	35.746204400317836
5	14.398431001538704	36.92840115761472	33.2779019429112	15.395265897935376
6	33.94518330917203	35.898926494290976	16.642527515310647	13.51336268122634
7	30.291383530299687	30.52982930763691	20.065255347590032	19.113531814473372
8	28.424676646531537	33.22993374808327	18.934481558155788	19.410908047229405
9	27.213180756382695	14.236521734508663	18.61717394250726	39.93312356660138
10	15.963387354060657	27.046970992510445	31.346505328658758	25.643136324770143
11	37.1358868535256	21.646544828337642	21.641229792899644	19.576338525237116
12	24.283748670409782	24.066297138582332	28.89461141417906	22.755342776828822
13	29.36404272225485	20.052765014310733	25.157192173078812	25.426000090355604
14	23.304570133221365	19.863549752717976	25.274920208030487	31.556959906030173
15	25.485063421660364	27.047883154260933	22.095133819304742	25.371919604773964
16	25.621816340862384	26.424130001920076	23.56414356870126	24.38991008851627
17	23.634434520092164	26.205051409680273	25.263027816237965	24.897486253989598
18	24.259812910255388	25.961426027797707	25.761314411756892	24.017446650190013
19	25.899702623767247	25.121315683872318	25.158454493995343	23.820527198365095
20	25.6206819345996	24.999750856886216	25.278857582487962	24.10070962602622
21	26.23415122870332	24.256758731938845	25.084774815236084	24.424315224121756
22	25.51642213074456	24.94711539739191	25.202170660472877	24.334291811390653
23	24.60643797382742	24.66377395670768	25.462159247868836	25.267628821596066
24	24.858850902171618	25.52297252226694	24.99910307704369	24.61907349851775
25	24.8212814584496	24.84865396369821	25.21419649738234	25.11586808046985
26	24.565942290405584	25.456015551866017	25.720174041644828	24.25786811608357
27	24.93435896345628	25.28727919232292	25.199182011698458	24.579179832522343
28	24.57122014320234	25.136681365159326	25.486083382110536	24.8060151095278
29	24.751504328119925	25.20793511906067	25.419208760282153	24.621351792537258
30	24.69547723736365	25.34259226039802	25.638178989092058	24.32375151314627
31	24.9869282912968	25.13854018080482	24.834585581988346	25.039945945910034
32	24.7349433611268	24.92050626183437	24.926352855197155	25.41819752184168
33	24.656498540687675	24.619293218466332	25.519529140072063	25.204679100773937
34	25.0121084518655	25.126582320530815	25.64380754836571	24.217501679237973
35	25.044646416327833	24.782946663477638	25.558279518456512	24.61412740173802
36	24.342578634445086	25.17511415722305	25.28460410547443	25.19770310285743
37	25.37134542748657	24.85617569188146	24.755453829722914	25.01702505090905
38	24.706211416164617	25.095139134340176	25.339962954203	24.858686495292208
39	25.41087239752025	24.61261361816854	25.21388219520832	24.762631789102887
40	25.36856497838771	24.965419004731718	25.30498378247657	24.361032234404004
41	24.67134439360408	25.37240548597579	25.497509545579266	24.458740574840864
42	25.430548763634043	25.29985595220377	25.068967533433973	24.200627750728213
43	24.92497121432686	24.749234098448664	25.31398475438994	25.011809932834538
44	24.78187495847452	25.154009700352137	25.079529599362164	24.984585741811173
45	24.98840628625112	25.142546205433963	25.00621210450155	24.862835403813367
46	24.835594722554053	25.142878783248136	24.845760011480795	25.175766482717016
47	24.84247438132494	25.23107300790884	25.22801683816651	24.698435772599712
48	25.361706425139264	25.516780414168473	24.62148392652124	24.500029234171024
49	25.04310330508503	25.491909202348005	24.865972805883974	24.599014686682988
50	24.97604877147804	25.689536269667578	24.84529698858978	24.489117970264598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	113.0
2	40.0
3	122.5
4	205.0
5	213.5
6	222.0
7	195.0
8	168.0
9	223.0
10	278.0
11	489.5
12	701.0
13	1292.5
14	1884.0
15	2523.5
16	3163.0
17	3037.5
18	2912.0
19	2923.0
20	2934.0
21	3449.5
22	3965.0
23	4985.5
24	6006.0
25	7770.0
26	9534.0
27	12391.0
28	15248.0
29	17762.0
30	20276.0
31	23144.5
32	26013.0
33	30057.0
34	34101.0
35	39313.0
36	44525.0
37	50422.0
38	56319.0
39	62403.0
40	68487.0
41	75628.0
42	82769.0
43	84908.5
44	87048.0
45	93575.0
46	100102.0
47	104452.0
48	108802.0
49	112646.0
50	116490.0
51	114730.0
52	112970.0
53	111245.0
54	109520.0
55	106955.5
56	104391.0
57	101321.5
58	98252.0
59	89554.5
60	80857.0
61	71024.5
62	61192.0
63	53175.0
64	45158.0
65	38096.5
66	31035.0
67	26665.0
68	22295.0
69	19861.0
70	17427.0
71	14019.5
72	10612.0
73	9103.5
74	7595.0
75	5917.0
76	4239.0
77	3639.0
78	3039.0
79	2636.0
80	2233.0
81	1571.5
82	910.0
83	713.0
84	516.0
85	417.0
86	318.0
87	216.5
88	115.0
89	73.5
90	32.0
91	29.5
92	27.0
93	20.0
94	13.0
95	13.0
96	13.0
97	18.0
98	23.0
99	13.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03414910268914218
2	0.003986276578499088
3	6.643794297498479E-4
4	0.0
5	0.0
6	1.3287588594996957E-4
7	0.0
8	0.0
9	0.0
10	6.643794297498479E-5
11	0.0
12	6.643794297498479E-5
13	0.0
14	0.0
15	0.0
16	0.0011294450305747413
17	0.0
18	2.6575177189993915E-4
19	0.0
20	3.3218971487492395E-4
21	0.0
22	0.0
23	6.643794297498479E-5
24	0.0011958829735497262
25	2.6575177189993915E-4
26	4.6506560082489355E-4
27	5.315035437998783E-4
28	0.0013951968024746805
29	4.6506560082489355E-4
30	0.0014616347454496652
31	3.3218971487492395E-4
32	9.301312016497871E-4
33	1.9931382892495435E-4
34	0.0011294450305747413
35	2.6575177189993915E-4
36	1.9931382892495435E-4
37	0.0019267003462745587
38	0.0
39	8.636932586748023E-4
40	1.3287588594996957E-4
41	0.0011294450305747413
42	0.007175297841298356
43	0.004982845723123859
44	0.004252028350399026
45	0.0025246418330494218
46	0.002790393604949361
47	7.972553156998174E-4
48	0.0050492836660988435
49	0.003919838635524102
50	0.0014616347454496652
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1505164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.08746187662946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15303901150521	37.58268668794736
2	15.744408985357278	16.401726055897193
3	5.5559137301103	8.681803338208873
4	2.3297390274115797	4.8540077109118585
5	1.1892425805733993	3.0972313788840693
6	0.698480701613649	2.1829252150117404
7	0.44204464243467495	1.6117488422409103
8	0.3150778051070483	1.3129282529348374
9	0.2215877309904253	1.0387748241263342
>10	1.1811066066624505	11.169843976566671
>50	0.0976353074991341	3.5373457816098166
>100	0.06697656822438768	6.792239063283326
>500	0.004490691564091936	1.5942550429273532
>1k	2.5661094651953923E-4	0.14248382944963814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.643794297498479E-5
2	0.0	0.0	0.0	0.0	6.643794297498479E-5
3	0.0	0.0	0.0	0.0	6.643794297498479E-5
4	0.0	0.0	0.0	6.643794297498479E-5	6.643794297498479E-5
5	0.0	0.0	0.0	6.643794297498479E-5	6.643794297498479E-5
6	0.0	0.0	0.0	6.643794297498479E-5	1.3287588594996957E-4
7	0.0	0.0	0.0	6.643794297498479E-5	1.3287588594996957E-4
8	0.0	0.0	0.0	6.643794297498479E-5	1.3287588594996957E-4
9	0.0	0.0	0.0	6.643794297498479E-5	1.3287588594996957E-4
10	0.0	0.0	0.0	6.643794297498479E-5	1.3287588594996957E-4
11	0.0	0.0	0.0	6.643794297498479E-5	1.3287588594996957E-4
12	0.0	0.0	0.0	6.643794297498479E-5	5.979414867748631E-4
13	0.0	0.0	0.0	1.3287588594996957E-4	7.972553156998174E-4
14	0.0	0.0	0.0	1.3287588594996957E-4	7.972553156998174E-4
15	0.0	0.0	0.0	1.9931382892495435E-4	9.30131201649787E-4
16	0.0	0.0	0.0	1.9931382892495435E-4	9.30131201649787E-4
17	0.0	0.0	0.0	1.9931382892495435E-4	9.30131201649787E-4
18	0.0	0.0	0.0	1.9931382892495435E-4	9.965691446247717E-4
19	0.0	0.0	0.0	3.986276578499087E-4	9.965691446247717E-4
20	0.0	0.0	0.0	5.315035437998783E-4	0.0010630070875997566
21	0.0	0.0	0.0	7.308173727248327E-4	0.0010630070875997566
22	0.0	0.0	0.0	9.30131201649787E-4	0.0011294450305747413
23	0.0	0.0	0.0	0.00152807268842465	0.0011294450305747413
24	0.0	0.0	0.0	0.002192452118174498	0.0011294450305747413
25	0.0	0.0	0.0	0.0029232694908993308	0.0011294450305747413
26	0.0	0.0	0.0	0.0032554592057742545	0.0013951968024746805
27	0.0	0.0	0.0	0.0038534006925491178	0.0014616347454496654
28	0.0	0.0	0.0	0.0045842180652739505	0.0014616347454496654
29	0.0	0.0	0.0	0.0058465389817986615	0.0014616347454496654
30	0.0	0.0	0.0	0.007108859898323372	0.0014616347454496654
31	0.0	0.0	0.0	0.009832815560297749	0.0014616347454496654
32	0.0	0.0	0.0	0.013819092138796835	0.0014616347454496654
33	0.0	0.0	0.0	0.01694167545862112	0.0014616347454496654
34	0.0	0.0	0.0	0.02152589352389507	0.0014616347454496654
35	0.0	0.0	0.0	0.025512170102394156	0.0014616347454496654
36	0.0	0.0	0.0	0.03301965765856744	0.0014616347454496654
37	0.0	0.0	0.0	0.042254531732090324	0.0014616347454496654
38	0.0	0.0	0.0	0.055209930612212355	0.0014616347454496654
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	915	0.0	28.85989	1
GTATCAA	2435	0.0	27.29245	1
CTAGCGG	250	0.0	19.359196	29
TCAACGC	3420	0.0	19.23312	4
ATCAACG	3490	0.0	18.847355	3
AACGCAG	3590	0.0	18.383638	6
TATCAAC	3850	0.0	17.543295	2
CAACGCA	3765	0.0	17.470724	5
TACGAAT	265	0.0	17.436134	41
GTATTAA	330	0.0	17.337797	1
CGCAATA	270	0.0	17.112106	36
GATATAC	285	0.0	16.98683	1
TAGGACG	235	0.0	16.850365	4
TTAGGAC	290	0.0	16.688961	3
ATACGAA	265	0.0	16.605843	40
TAGCGGC	305	0.0	16.589476	30
TAGGACA	320	0.0	16.499315	4
GCGCTAT	120	6.4111628E-6	16.499315	11
TCTAGCG	280	0.0	16.499313	28
TACTAGA	135	1.0855947E-6	16.296703	2
>>END_MODULE