FastQCFastQC Report
Thu 2 Feb 2017
SRR4061986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609057
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22560.14020634446138328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGC400.005273182414.2524529
GGTATCA6900.013.7627721
ATAAACC1001.4451507E-712.3482813
GTATCAA17850.012.1829441
AGAACCG658.018388E-411.6932285
CCAATCG600.00587781111.08282515
GTCTTAC600.005892809511.0790311
TCTAGAA1553.1468517E-1011.0296932
CGTGCCA951.3575707E-511.00189210
TCGTACA700.001491245110.8573224
TCTAGGA2550.010.8014092
GTGCTAG1951.8189894E-1210.7137881
GTCTAGG803.784376E-410.68335151
TAGACAG1803.45608E-1110.5563865
TCAGCCG909.5130286E-510.55572913
CCCTAGA3400.010.3342221
CTAGGAC2400.010.2902343
ACCGTGC1409.6648364E-810.1800068
CGTGCGC750.002643877210.135076510
GCCGTGT750.002644560310.1347628
Produced by FastQC (version 0.11.5)