##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1609057
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2745216608237	32.0	32.0	32.0	32.0	32.0
2	31.408748726738704	32.0	32.0	32.0	32.0	32.0
3	31.538952939516747	32.0	32.0	32.0	32.0	32.0
4	31.628279793692826	32.0	32.0	32.0	32.0	32.0
5	31.597103769474916	32.0	32.0	32.0	32.0	32.0
6	35.22799875952188	36.0	36.0	36.0	36.0	36.0
7	35.23184076139005	36.0	36.0	36.0	36.0	36.0
8	35.18667144793503	36.0	36.0	36.0	36.0	36.0
9	35.28087009969193	36.0	36.0	36.0	36.0	36.0
10	35.15306853641605	36.0	36.0	36.0	36.0	36.0
11	35.2754756357295	36.0	36.0	36.0	36.0	36.0
12	35.208130600718306	36.0	36.0	36.0	36.0	36.0
13	35.23526574882058	36.0	36.0	36.0	36.0	36.0
14	35.20389955110353	36.0	36.0	36.0	36.0	36.0
15	35.174622154466874	36.0	36.0	36.0	36.0	36.0
16	35.18741971229111	36.0	36.0	36.0	36.0	36.0
17	35.174410850578944	36.0	36.0	36.0	36.0	36.0
18	35.168723668583524	36.0	36.0	36.0	36.0	36.0
19	35.16165306760419	36.0	36.0	36.0	36.0	36.0
20	35.159254768476195	36.0	36.0	36.0	36.0	36.0
21	35.14953727555954	36.0	36.0	36.0	36.0	36.0
22	35.13036579810411	36.0	36.0	36.0	36.0	36.0
23	35.1059931375955	36.0	36.0	36.0	36.0	36.0
24	35.091392660421604	36.0	36.0	36.0	36.0	36.0
25	35.08114317889298	36.0	36.0	36.0	36.0	36.0
26	35.03555312210817	36.0	36.0	36.0	36.0	36.0
27	35.00627634695353	36.0	36.0	36.0	36.0	36.0
28	35.000590407922154	36.0	36.0	36.0	36.0	36.0
29	34.97962906223956	36.0	36.0	36.0	36.0	36.0
30	34.95941784535911	36.0	36.0	36.0	36.0	36.0
31	34.95600963794322	36.0	36.0	36.0	36.0	36.0
32	34.92415557683786	36.0	36.0	36.0	36.0	36.0
33	34.90999510893648	36.0	36.0	36.0	32.0	36.0
34	34.90834569564658	36.0	36.0	36.0	36.0	36.0
35	34.88489034260439	36.0	36.0	36.0	32.0	36.0
36	34.85638855553284	36.0	36.0	36.0	32.0	36.0
37	34.85413444023425	36.0	36.0	36.0	32.0	36.0
38	34.847012256246984	36.0	36.0	36.0	32.0	36.0
39	34.82795326703778	36.0	36.0	36.0	32.0	36.0
40	34.815023955024586	36.0	36.0	36.0	32.0	36.0
41	34.78674465851738	36.0	36.0	36.0	32.0	36.0
42	34.75868412368238	36.0	36.0	36.0	32.0	36.0
43	34.75032146157656	36.0	36.0	36.0	32.0	36.0
44	34.717056636278265	36.0	36.0	36.0	32.0	36.0
45	34.69644953534897	36.0	36.0	36.0	32.0	36.0
46	34.68426786620984	36.0	36.0	36.0	32.0	36.0
47	34.65459582848836	36.0	36.0	36.0	32.0	36.0
48	34.621771633944604	36.0	36.0	36.0	32.0	36.0
49	34.61025308612436	36.0	36.0	36.0	32.0	36.0
50	34.194238613051	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	15.0
21	34.0
22	107.0
23	316.0
24	730.0
25	1880.0
26	3865.0
27	7440.0
28	12825.0
29	20634.0
30	30425.0
31	45452.0
32	68582.0
33	116588.0
34	267861.0
35	1032297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64633670974372	17.763797992307182	12.576398201320101	26.013467096629
2	16.163087071879655	19.699424666916514	37.47987080021454	26.657617460989286
3	18.987169487680656	23.03457619534447	28.292755685790727	29.685498631184142
4	13.310529086290915	16.040015984517638	34.27063180483973	36.37882312435172
5	14.962241859673089	36.420835309128265	33.056628820483056	15.560294010715591
6	35.38103755374276	34.19139444667488	16.567747260191393	13.85982073939097
7	30.95110987367135	29.993157482923227	19.867475173346875	19.188257470058552
8	28.071410770407763	33.91122875075277	18.519480664762032	19.497879814077436
9	26.959455134280514	15.466139484182351	18.383438249856905	39.19096713168023
10	16.90984222435874	27.11718727179957	30.99231413181758	24.98065637202411
11	36.62486785738479	21.741864955685227	22.01743008482608	19.615837102103903
12	24.671655510028543	23.711217191187135	28.363134432154986	23.253992866629336
13	29.506039873043655	20.045902662242543	24.793155245587943	25.65490221912586
14	23.557773279629	19.369978813677825	25.012351955213518	32.05989595147965
15	25.074002971927033	27.1189895696672	21.875732183508724	25.931275274897036
16	25.644606362535765	26.155841605325897	23.113911117408847	25.08564091472949
17	24.17347552013384	26.20273862268397	24.969531843806653	24.65425401337554
18	24.56079838116262	26.123391603524055	25.2305078257065	24.085302189606825
19	25.647444434846	25.439620846247212	25.03777056996738	23.875164148939408
20	25.914189064472243	25.017323897532016	24.797256018927953	24.271231019067784
21	25.920026450274914	24.695271826914773	24.794336061432254	24.590365661378062
22	25.65993622351477	24.473029855374918	25.060578960223285	24.806454960887027
23	25.004179471801773	24.84029445854865	25.25215110732697	24.903374962322605
24	24.95345062036837	25.043566241818123	25.048103097242837	24.954880040570675
25	25.09045687714645	24.786551601127123	25.145768880348328	24.977222641378102
26	24.987585881153844	24.991874112444883	25.160482394946104	24.860057611455172
27	25.155215424715394	25.213448398872877	24.895621899575776	24.735714276835953
28	25.54207822184546	24.713571268316056	25.072294705100372	24.672055804738108
29	24.9193468696766	25.06558213505973	25.14780451334358	24.86726648192009
30	25.1764875512496	25.16592222552993	24.789734480934868	24.867855742285602
31	25.246278401022217	24.990848654457	24.628834475930876	25.13403846858991
32	24.97108533322561	25.374678769083523	24.898495691543744	24.755740206147124
33	25.08348067654617	25.14451028709398	24.83942438263453	24.932584653725325
34	25.23630447090731	24.833768062710412	25.18633700569343	24.743590460688843
35	25.058264193157452	24.87411826854355	25.266772319070256	24.800845219228737
36	25.306049392997377	25.0954287426028	24.821789697548997	24.77673216685083
37	25.353676916144867	24.68389241932226	25.08612358415811	24.876307080374758
38	25.145473404609035	25.08307660946753	24.922796395652856	24.848653590270576
39	25.19262967000322	25.221777376159537	24.886547681048274	24.699045272788968
40	25.257589401965006	25.202898847955908	24.767301014944813	24.772210735134266
41	25.10096069586748	25.175849977812888	24.960442300449586	24.762747025870052
42	25.283563685451583	25.160318361999423	24.831415562347964	24.724702390201035
43	25.192496415455402	24.75843556614052	24.90722446551907	25.141843552885007
44	25.067200166065984	24.91424792681661	24.92773457286176	25.090817334255643
45	25.091033842401917	25.076615101021304	24.978107751502936	24.854243305073844
46	25.29931262507613	24.941765795328834	24.76743607910405	24.99148550049098
47	25.023942803268774	25.10486046674949	24.99982909095655	24.871367639025184
48	25.043691842614894	25.339093447093713	24.611372557793803	25.005842152497582
49	25.024999953387283	24.948057896722382	24.863844256350053	25.163097893540286
50	24.88256929312867	25.257949800936462	24.89220240218665	24.96727850374821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	16.0
2	28.0
3	65.0
4	102.0
5	119.0
6	136.0
7	129.5
8	123.0
9	159.5
10	196.0
11	315.5
12	435.0
13	810.0
14	1185.0
15	1522.5
16	1860.0
17	1872.0
18	1884.0
19	1804.5
20	1725.0
21	2129.0
22	2533.0
23	3335.5
24	4138.0
25	5688.0
26	7238.0
27	9598.5
28	11959.0
29	14711.0
30	17463.0
31	21256.5
32	25050.0
33	29580.0
34	34110.0
35	39345.5
36	44581.0
37	50779.5
38	56978.0
39	64435.0
40	71892.0
41	77849.5
42	83807.0
43	88222.5
44	92638.0
45	100789.5
46	108941.0
47	114138.0
48	119335.0
49	121471.5
50	123608.0
51	127165.5
52	130723.0
53	131985.0
54	133247.0
55	129943.0
56	126639.0
57	119164.0
58	111689.0
59	102670.0
60	93651.0
61	80532.0
62	67413.0
63	57322.0
64	47231.0
65	39544.0
66	31857.0
67	26310.0
68	20763.0
69	16897.0
70	13031.0
71	10446.0
72	7861.0
73	6473.0
74	5085.0
75	4047.0
76	3009.0
77	2449.0
78	1889.0
79	1654.5
80	1420.0
81	1153.5
82	887.0
83	614.0
84	341.0
85	272.5
86	204.0
87	146.5
88	89.0
89	55.5
90	22.0
91	18.0
92	14.0
93	10.5
94	7.0
95	11.5
96	16.0
97	13.0
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03380862206870235
2	0.004723263377245181
3	1.2429640466434688E-4
4	0.0
5	0.0
6	1.8644460699652031E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.079266303182548E-4
17	0.0
18	6.214820233217344E-5
19	0.0
20	4.971856186573875E-4
21	0.0
22	0.0
23	1.2429640466434688E-4
24	8.079266303182548E-4
25	1.8644460699652031E-4
26	1.2429640466434688E-4
27	1.8644460699652031E-4
28	0.001118667641979122
29	3.7288921399304063E-4
30	0.0012429640466434686
31	2.4859280932869375E-4
32	7.457784279860813E-4
33	1.2429640466434688E-4
34	6.214820233217343E-4
35	4.350374163252141E-4
36	1.8644460699652031E-4
37	0.00198874247462955
38	0.0
39	6.836302256539078E-4
40	2.4859280932869375E-4
41	9.322230349826016E-4
42	0.004412522365584314
43	0.004101781353923447
44	0.0036045957352660598
45	0.0024859280932869373
46	0.0022994834862904174
47	8.700748326504282E-4
48	0.0037910403422625797
49	0.0033560029259373657
50	0.0014294086536399892
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1609057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.89506038158367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.560834799960624	20.302545803773654
2	16.088956201291484	11.55028108644026
3	8.45092778939601	9.100396898423195
4	5.076776899263722	7.289248533716017
5	3.319791464613911	5.958205753829122
6	2.2617287576020377	4.871093419253363
7	1.672629724578603	4.2027401471846435
8	1.197704478714674	3.4393339666205134
9	0.9132793381066728	2.950399528793262
>10	4.302050711947369	25.21179440125897
>50	0.11878195706073118	2.874976576709722
>100	0.035841950878246015	2.092669488886718
>500	6.959265859967144E-4	0.1563143951105753
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.214820233217344E-5	0.0
11	0.0	0.0	0.0	6.214820233217344E-5	0.0
12	0.0	0.0	0.0	6.214820233217344E-5	0.0
13	0.0	0.0	0.0	6.214820233217344E-5	0.0
14	0.0	0.0	0.0	1.2429640466434688E-4	0.0
15	0.0	0.0	0.0	1.8644460699652031E-4	0.0
16	0.0	0.0	0.0	3.107410116608672E-4	0.0
17	0.0	0.0	0.0	4.350374163252141E-4	0.0
18	0.0	0.0	0.0	5.593338209895609E-4	0.0
19	0.0	0.0	0.0	9.94371237314775E-4	0.0
20	0.0	0.0	0.0	0.0012429640466434689	0.0
21	0.0	0.0	0.0	0.0017401496653008564	0.0
22	0.0	0.0	0.0	0.0021751870816260704	0.0
23	0.0	0.0	0.0	0.0033560029259373657	0.0
24	0.0	0.0	0.0	0.004723263377245181	0.0
25	0.0	0.0	0.0	0.00577978281689213	0.0
26	0.0	0.0	0.0	0.008017118100850373	0.0
27	0.0	0.0	0.0	0.00957082315915471	0.0
28	0.0	0.0	0.0	0.011310972824455567	0.0
29	0.0	0.0	0.0	0.013796900917742504	0.0
30	0.0	0.0	0.0	0.016344977213361613	0.0
31	0.0	0.0	0.0	0.01858231249731986	0.0
32	0.0	0.0	0.0	0.022870538458239827	0.0
33	0.0	0.0	0.0	0.02653728239583806	0.0
34	0.0	0.0	0.0	0.03250350981972671	0.0
35	0.0	0.0	0.0	0.036916032185311025	0.0
36	0.0	0.0	0.0	0.04275796320453533	0.0
37	0.0	0.0	0.0	0.052515230970686555	0.0
38	0.0	0.0	0.0	0.06252109154616649	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCTA	25	0.0023536435	35.19811	23
GGTATCA	740	0.0	26.76863	1
GTATCAA	1420	0.0	22.629755	1
CGGATCG	95	1.5979567E-5	18.525898	26
ATAGGGG	180	1.4551915E-11	18.33292	3
GTATAGG	270	0.0	17.933882	1
TAGGGGT	235	0.0	16.85016	4
GTATATA	170	2.4556357E-9	16.830994	1
ATCGATT	145	1.2503006E-7	16.689278	29
CCTACAC	225	2.5465852E-11	15.644091	3
TATCAAC	2060	0.0	15.593334	2
CAACCGC	100	4.946976E-4	15.399173	19
GTTTATA	225	4.2564352E-10	14.673175	1
GTTATAG	210	2.5283953E-9	14.673175	1
CGACTCA	90	0.004355995	14.668614	42
TACTGCG	135	1.9577607E-5	14.66588	7
TTACACC	380	0.0	14.472907	4
TATAGGG	275	3.6379788E-12	14.401018	2
TCAACGC	2200	0.0	14.199239	4
ATCAACG	2220	0.0	14.071753	3
>>END_MODULE