FastQCFastQC Report
Thu 2 Feb 2017
SRR4061985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696128
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22910.13507235302996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA453.541407E-516.88765514
TATACCC456.7544106E-414.78018955
CTAGCAC653.376148E-614.6138863
GCGACGC400.00528088614.24937913
GGTATCA5300.013.6164211
GTATCAA14200.013.0398181
CCGTTCA951.0378972E-612.0019599
AGTTTCG658.028993E-411.69145416
GTATTAC1552.5465852E-1111.6398441
CTGCGTT600.005866316611.08579611
GTCCGGT600.005866316611.08579611
CGACCAT600.005867606511.08546910
TACTAGC600.005883100411.0815422
TAGGACG2350.010.9153854
TAGGACA2100.010.8576334
GCCGATT803.769481E-410.68798212
CCGATTG803.7725674E-410.68703513
TTTCGAC803.7735968E-410.68671918
TAGCACT2001.8189894E-1210.4504714
AGGACGT2850.010.3350195
Produced by FastQC (version 0.11.5)