FastQCFastQC Report
Thu 2 Feb 2017
SRR4061985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696128
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15000.026.2645441
GGTATCA6850.025.0617921
TATCGCA856.1150804E-620.70617315
TTCGACG550.00448397920.00028212
TAGCGCC550.0044855119.9991024
ATAGACG2250.019.5546783
TATCAAC21750.018.3072242
TCAACGC22350.017.6188964
ATCAACG22500.017.599213
GTTATAT1154.257581E-617.2247431
CAACGCA23200.016.8785535
CGTCGTA1053.776289E-516.76164610
AACGCAG23850.016.418556
ATACCGT1906.184564E-1016.2097976
GTCCTAA3050.015.8756281
ACCGTAT1401.5877904E-615.7135818
TAGGACA3650.015.6705284
ATAGCGT850.002981494615.5287156
CTAGCGG1004.9457327E-415.39976329
GCGTGTC1004.9468403E-415.3993099
Produced by FastQC (version 0.11.5)