##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696128
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131748901026338	32.0	32.0	32.0	32.0	32.0
2	31.0661058599351	32.0	32.0	32.0	32.0	32.0
3	31.351468167496794	32.0	32.0	32.0	32.0	32.0
4	31.498965290355446	32.0	32.0	32.0	32.0	32.0
5	31.413768890083766	32.0	32.0	32.0	32.0	32.0
6	35.04171442249641	36.0	36.0	36.0	36.0	36.0
7	35.05929976982869	36.0	36.0	36.0	36.0	36.0
8	34.9875581324051	36.0	36.0	36.0	36.0	36.0
9	35.13282134367218	36.0	36.0	36.0	36.0	36.0
10	34.94243358991774	36.0	36.0	36.0	32.0	36.0
11	35.134589488529166	36.0	36.0	36.0	36.0	36.0
12	35.03919692381707	36.0	36.0	36.0	36.0	36.0
13	35.08337637253792	36.0	36.0	36.0	36.0	36.0
14	35.03708859237039	36.0	36.0	36.0	36.0	36.0
15	34.99087981567429	36.0	36.0	36.0	36.0	36.0
16	35.02362321711569	36.0	36.0	36.0	36.0	36.0
17	34.9991309618142	36.0	36.0	36.0	36.0	36.0
18	34.997339823409554	36.0	36.0	36.0	36.0	36.0
19	34.99229185533167	36.0	36.0	36.0	36.0	36.0
20	34.98930858991774	36.0	36.0	36.0	36.0	36.0
21	34.97160709569089	36.0	36.0	36.0	36.0	36.0
22	34.95643783959701	36.0	36.0	36.0	32.0	36.0
23	34.917455522224735	36.0	36.0	36.0	32.0	36.0
24	34.906144465512035	36.0	36.0	36.0	32.0	36.0
25	34.88362729699645	36.0	36.0	36.0	32.0	36.0
26	34.83582430099615	36.0	36.0	36.0	32.0	36.0
27	34.81336726945136	36.0	36.0	36.0	32.0	36.0
28	34.788761815146025	36.0	36.0	36.0	32.0	36.0
29	34.76788367387367	36.0	36.0	36.0	32.0	36.0
30	34.75005483076749	36.0	36.0	36.0	32.0	36.0
31	34.73670855029809	36.0	36.0	36.0	32.0	36.0
32	34.710646248396344	36.0	36.0	36.0	32.0	36.0
33	34.681528752546974	36.0	36.0	36.0	32.0	36.0
34	34.66348412383971	36.0	36.0	36.0	32.0	36.0
35	34.65762017960908	36.0	36.0	36.0	32.0	36.0
36	34.62095372519055	36.0	36.0	36.0	32.0	36.0
37	34.61230225549015	36.0	36.0	36.0	32.0	36.0
38	34.59514317315674	36.0	36.0	36.0	32.0	36.0
39	34.56831618840088	36.0	36.0	36.0	32.0	36.0
40	34.56697666685533	36.0	36.0	36.0	32.0	36.0
41	34.533241005395816	36.0	36.0	36.0	32.0	36.0
42	34.51148851973436	36.0	36.0	36.0	32.0	36.0
43	34.49069822560561	36.0	36.0	36.0	32.0	36.0
44	34.44726577239454	36.0	36.0	36.0	32.0	36.0
45	34.42905193475964	36.0	36.0	36.0	32.0	36.0
46	34.408435566183684	36.0	36.0	36.0	32.0	36.0
47	34.38415614859256	36.0	36.0	36.0	32.0	36.0
48	34.35604801052751	36.0	36.0	36.0	32.0	36.0
49	34.338600624481174	36.0	36.0	36.0	32.0	36.0
50	33.831161327446985	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	40.0
22	103.0
23	289.0
24	775.0
25	1765.0
26	3962.0
27	8059.0
28	14405.0
29	24548.0
30	38411.0
31	59721.0
32	94575.0
33	167313.0
34	368113.0
35	914037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38792309819966	17.364711488502415	12.589694488790807	26.657670924507126
2	16.388571159039184	19.8426352841291	36.680777802672075	27.08801575415964
3	18.94236506293172	22.4823185097316	28.46761203779912	30.10770438953756
4	13.386312825447137	16.23220653158252	34.67786629310996	35.70361434986039
5	15.404085069051392	35.69376839483812	32.930769375896155	15.971377160214322
6	35.4306261986896	34.314433564075244	16.30972517912911	13.945215058106045
7	31.248820843709908	29.787551411214245	19.711307165496944	19.2523205795789
8	28.16473756697608	33.58590861067089	18.593584918119387	19.655768904233643
9	27.26085531280658	14.880893423137875	17.96556627801675	39.89268498603879
10	16.413462207406763	27.140141711170042	31.28600115793284	25.160394923490355
11	36.74227416798732	21.212255207154175	21.8143324088748	20.231138215983698
12	24.708276733831408	23.124905667496794	28.68722171911554	23.479595879556257
13	29.966311504792092	19.67982369255151	24.386838729152515	25.967026073503884
14	23.572631310844464	19.126445645611653	25.03419553241265	32.266727511131236
15	25.373910459587957	26.80575994264584	21.785325164138555	26.035004433627652
16	25.70872174502113	25.862425764260365	23.423502034352584	25.00535045636592
17	24.560056788166932	25.725652780922193	24.94033469172138	24.773955739189496
18	24.746038914561773	25.670380620307686	25.383196767221307	24.200383697909235
19	25.949869349483055	24.807090030941062	24.918933005056225	24.324107614519658
20	25.727040861447463	24.83282452559427	24.936649604214793	24.503485008743475
21	26.13937517650506	24.262393087310087	24.648625957843958	24.949605778340892
22	25.60667591125198	24.461833069202324	24.960439306467435	24.971051713078257
23	25.33275790506377	24.593898573692552	24.73162402837522	25.341719492868464
24	24.918386923512763	24.890499630626024	24.924400673775864	25.26671277208535
25	25.416066278173062	24.448978966160492	24.980308043515688	25.154646712150765
26	25.147424536678376	24.95634158391908	25.01400253047835	24.882231348924194
27	25.251072741229702	24.857233147143898	24.906639970473822	24.98505414115258
28	25.226798513538927	24.857246792511567	24.956827656713102	24.959127037236403
29	25.16144209221169	24.99671308440033	24.632292899260015	25.209551924127965
30	25.34524527552115	24.96897317572591	24.729838165263754	24.955943383489192
31	25.368444077331837	24.71896134646213	24.655050736416488	25.25754383978954
32	25.2918879815201	24.925461923822294	24.730191915279228	25.052458179378377
33	25.32830697719391	24.706065469192737	24.759717143655603	25.20591040995775
34	25.22638527032102	24.89226838070341	24.877410880053393	25.00393546892218
35	25.195578386708984	24.858751399515246	25.042405533089283	24.903264680686483
36	25.36313698947541	25.020591077415965	24.750917238903074	24.86535469420555
37	25.44285550042893	24.637888797502498	24.79542714293716	25.123828559131418
38	25.365774281186326	24.86592992981662	24.792409535129426	24.975886253867632
39	25.736492792638227	24.70867212267107	24.5442962821463	25.010538802544406
40	25.677234873608963	24.757255508880537	24.668346967352054	24.89716265015845
41	25.082527125320365	24.874993293473473	25.073152727802285	24.969326853403878
42	25.476653598994837	24.758629350788866	24.79889955620229	24.965817494014008
43	25.356413437228788	24.52342600279235	24.91450794309649	25.20565261688238
44	25.118097237126047	24.8159263233612	24.790514486515807	25.27546195299695
45	25.255793605778926	24.983520960512802	24.673927270540432	25.086758163167843
46	25.29849624281802	24.75506828961992	24.588272403800516	25.358163063761545
47	25.282897836044544	24.75887429271856	24.83375164420641	25.12447622703049
48	25.308316966999456	24.926963208324217	24.440188837325028	25.324530987351295
49	25.36634604386365	24.759886655888835	24.61449205636075	25.259275243886766
50	25.444505303051297	24.9207151233507	24.411664649292288	25.22311492430572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	7.5
2	14.0
3	39.5
4	65.0
5	76.5
6	88.0
7	90.0
8	92.0
9	108.0
10	124.0
11	219.0
12	314.0
13	627.0
14	940.0
15	1354.5
16	1769.0
17	1792.5
18	1816.0
19	1920.0
20	2024.0
21	2510.5
22	2997.0
23	3980.0
24	4963.0
25	6497.0
26	8031.0
27	10410.0
28	12789.0
29	16146.0
30	19503.0
31	23586.0
32	27669.0
33	32322.5
34	36976.0
35	42570.0
36	48164.0
37	53289.5
38	58415.0
39	64051.0
40	69687.0
41	77361.5
42	85036.0
43	90171.0
44	95306.0
45	102763.0
46	110220.0
47	115350.5
48	120481.0
49	125662.5
50	130844.0
51	132742.5
52	134641.0
53	134964.0
54	135287.0
55	131880.5
56	128474.0
57	122092.5
58	115711.0
59	106223.0
60	96735.0
61	85721.5
62	74708.0
63	65133.5
64	55559.0
65	46933.5
66	38308.0
67	32400.0
68	26492.0
69	22442.5
70	18393.0
71	15131.0
72	11869.0
73	10036.0
74	8203.0
75	6621.5
76	5040.0
77	4203.5
78	3367.0
79	2866.0
80	2365.0
81	1854.5
82	1344.0
83	1012.5
84	681.0
85	486.5
86	292.0
87	227.5
88	163.0
89	118.5
90	74.0
91	51.5
92	29.0
93	23.0
94	17.0
95	19.5
96	22.0
97	20.5
98	19.0
99	13.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03012744321183307
2	0.004009131386310467
3	2.3583125801826277E-4
4	0.0
5	0.0
6	2.9478907252282845E-4
7	0.0
8	0.0
9	0.0
10	1.1791562900913139E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.485359595502226E-4
17	0.0
18	1.1791562900913139E-4
19	0.0
20	3.5374688702739413E-4
21	5.8957814504565693E-5
22	0.0
23	0.0
24	8.843672175684853E-4
25	2.3583125801826277E-4
26	3.5374688702739413E-4
27	3.5374688702739413E-4
28	0.0011201984755867481
29	5.306203305410912E-4
30	8.843672175684853E-4
31	5.306203305410912E-4
32	8.843672175684853E-4
33	1.1791562900913139E-4
34	8.843672175684853E-4
35	2.9478907252282845E-4
36	2.9478907252282845E-4
37	0.0019456078786506679
38	0.0
39	8.843672175684853E-4
40	1.7687344351369707E-4
41	0.0011201984755867481
42	0.005011414232888084
43	0.0037733001282922044
44	0.004009131386310467
45	0.0020045656931552336
46	0.002535186023696325
47	0.0012381141045958795
48	0.00383225794279677
49	0.0031837219832465476
50	0.0017097766206324052
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1696128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.56829559559784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.53885125807048	26.811428622251427
2	16.499373571421945	14.376991698038122
3	7.8612693500755	10.275063204021082
4	4.332200406835489	7.549863516175112
5	2.6371911369877488	5.74489614991865
6	1.7842820063558074	4.664287552728973
7	1.2101609411789374	3.690725472247159
8	0.8482429813633854	2.9565200759144936
9	0.6292625198564654	2.4674305925103517
>10	2.566844074476467	17.957734567893652
>50	0.07256047739522116	2.1178393687820334
>100	0.019625010713742872	1.3555757929327223
>500	1.3626526886583104E-4	0.031643386586209556
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.8957814504565693E-5	0.0
10	0.0	0.0	0.0	5.8957814504565693E-5	0.0
11	0.0	0.0	0.0	5.8957814504565693E-5	0.0
12	0.0	0.0	0.0	5.8957814504565693E-5	0.0
13	0.0	0.0	0.0	5.8957814504565693E-5	0.0
14	0.0	0.0	0.0	5.8957814504565693E-5	0.0
15	0.0	0.0	0.0	5.8957814504565693E-5	0.0
16	0.0	0.0	0.0	5.8957814504565693E-5	0.0
17	0.0	0.0	0.0	5.8957814504565693E-5	0.0
18	0.0	0.0	0.0	1.1791562900913139E-4	0.0
19	0.0	0.0	0.0	1.1791562900913139E-4	0.0
20	0.0	0.0	0.0	1.7687344351369707E-4	0.0
21	0.0	0.0	0.0	3.5374688702739413E-4	0.0
22	0.0	0.0	0.0	3.5374688702739413E-4	0.0
23	0.0	0.0	0.0	4.7166251603652555E-4	0.0
24	0.0	0.0	0.0	5.895781450456569E-4	0.0
25	0.0	0.0	0.0	5.895781450456569E-4	0.0
26	0.0	0.0	0.0	8.254094030639197E-4	0.0
27	0.0	0.0	0.0	0.0012381141045958795	5.8957814504565693E-5
28	0.0	0.0	0.0	0.0015329031771187081	5.8957814504565693E-5
29	0.0	0.0	0.0	0.0019456078786506679	5.8957814504565693E-5
30	0.0	0.0	0.0	0.002181439136668931	5.8957814504565693E-5
31	0.0	0.0	0.0	0.0030658063542374162	5.8957814504565693E-5
32	0.0	0.0	0.0	0.004362878273337862	5.8957814504565693E-5
33	0.0	0.0	0.0	0.006780148668025055	5.8957814504565693E-5
34	0.0	0.0	0.0	0.008843672175684854	5.8957814504565693E-5
35	0.0	0.0	0.0	0.011555731642894876	5.8957814504565693E-5
36	0.0	0.0	0.0	0.01491632706965512	5.8957814504565693E-5
37	0.0	0.0	0.0	0.01916128971398385	5.8957814504565693E-5
38	0.0	0.0	0.0	0.025941438382008904	5.8957814504565693E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1500	0.0	26.264544	1
GGTATCA	685	0.0	25.061792	1
TATCGCA	85	6.1150804E-6	20.706173	15
TTCGACG	55	0.004483979	20.000282	12
TAGCGCC	55	0.00448551	19.999102	4
ATAGACG	225	0.0	19.554678	3
TATCAAC	2175	0.0	18.307224	2
TCAACGC	2235	0.0	17.618896	4
ATCAACG	2250	0.0	17.59921	3
GTTATAT	115	4.257581E-6	17.224743	1
CAACGCA	2320	0.0	16.878553	5
CGTCGTA	105	3.776289E-5	16.761646	10
AACGCAG	2385	0.0	16.41855	6
ATACCGT	190	6.184564E-10	16.209797	6
GTCCTAA	305	0.0	15.875628	1
ACCGTAT	140	1.5877904E-6	15.713581	8
TAGGACA	365	0.0	15.670528	4
ATAGCGT	85	0.0029814946	15.528715	6
CTAGCGG	100	4.9457327E-4	15.399763	29
GCGTGTC	100	4.9468403E-4	15.399309	9
>>END_MODULE