FastQCFastQC Report
Thu 2 Feb 2017
SRR4061984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794264
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62710.13080214189289535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48450.013.0717751
GGTATCA16450.013.045031
CGCGTAA1104.9649316E-711.2291810
TTAGCCG600.00587627911.0839424
GCGTTAT1900.010.9944011
CGTTATT1951.8189894E-1210.7155152
GTATTAG3150.010.5501831
CGAATTT1002.4022696E-510.44959315
TAGACAG5200.010.414475
CGACCAT3400.010.33999210
GTATAGT2300.010.3208311
AAGACGG5650.010.0894735
GTCTTAG5050.09.9652221
GGACCGT2107.2759576E-129.9536576
GTATAGG4400.09.7109641
GGCGTAA900.0011130999.5012186
TAGGCCG900.00111341839.5009195
CGAACCT2700.09.49943116
CAAGACG5650.09.4164484
GTATTAC2850.09.3285841
Produced by FastQC (version 0.11.5)