##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061984_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4794264
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258222325679185	32.0	32.0	32.0	32.0	32.0
2	30.832777669314833	32.0	32.0	32.0	32.0	32.0
3	30.850442111656765	32.0	32.0	32.0	32.0	32.0
4	30.85356209003092	32.0	32.0	32.0	32.0	32.0
5	30.785062733299625	32.0	32.0	32.0	32.0	32.0
6	34.42972039086709	36.0	36.0	36.0	32.0	36.0
7	34.36801561198966	36.0	36.0	36.0	32.0	36.0
8	34.34394080092377	36.0	36.0	36.0	32.0	36.0
9	34.456599177684	36.0	36.0	36.0	32.0	36.0
10	34.17588518279344	36.0	36.0	36.0	32.0	36.0
11	34.432478687031	36.0	36.0	36.0	32.0	36.0
12	34.283128755529525	36.0	36.0	36.0	32.0	36.0
13	34.34159174380051	36.0	36.0	36.0	32.0	36.0
14	34.26898456155105	36.0	36.0	36.0	32.0	36.0
15	34.22677829172528	36.0	36.0	36.0	32.0	36.0
16	34.23012666803497	36.0	36.0	36.0	32.0	36.0
17	34.17390865417507	36.0	36.0	36.0	32.0	36.0
18	34.169968946224074	36.0	36.0	36.0	32.0	36.0
19	34.177470619056436	36.0	36.0	36.0	32.0	36.0
20	34.155165214097515	36.0	36.0	36.0	32.0	36.0
21	34.14883202093168	36.0	36.0	36.0	32.0	36.0
22	34.11436812824659	36.0	36.0	36.0	32.0	36.0
23	34.08670694813635	36.0	36.0	36.0	32.0	36.0
24	34.06116246414465	36.0	36.0	36.0	32.0	36.0
25	33.71595556690245	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	66.0
5	402.0
6	934.0
7	188.0
8	834.0
9	685.0
10	382.0
11	113.0
12	175.0
13	214.0
14	638.0
15	915.0
16	1542.0
17	2067.0
18	2941.0
19	4044.0
20	6127.0
21	8807.0
22	13157.0
23	19417.0
24	27273.0
25	39158.0
26	53753.0
27	69420.0
28	92337.0
29	122287.0
30	161292.0
31	218865.0
32	310212.0
33	443531.0
34	983711.0
35	2208773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40424048743767	16.877285176941452	11.774549858339627	25.943924477281254
2	16.913476315785083	19.333292729120398	36.972075704052024	26.7811552510425
3	19.677666829640284	22.91620430550712	27.45148753752426	29.95464132732834
4	13.618017702290283	15.450098357646342	34.15628331174263	36.775600628320745
5	15.733534139814227	35.62069942583201	32.720689953040825	15.925076481312935
6	35.091781127838225	33.46927445427014	16.803366108083626	14.635578309808006
7	31.130095976463686	29.575137366509402	19.949720151298912	19.345046505727996
8	28.437608776497612	32.564512097669216	18.906621190825845	20.091257935007327
9	27.172090091862326	15.253606550085108	18.269678750209614	39.30462460784295
10	16.829388411855817	26.64319513576645	30.442291529864274	26.085124922513454
11	36.42623010400028	21.466280584587256	22.0215294439804	20.085959867432063
12	25.25609587228613	22.975392615591677	28.043278620320628	23.72523289180157
13	29.471930535015794	19.74088471533512	24.76989494248183	26.017289807167256
14	23.897059437714773	19.044012375687654	24.85643417146542	32.202494015132146
15	25.35456456516041	26.64502189366352	21.914967162115452	26.085446379060613
16	25.995233654110805	25.651310807768656	23.051768647618267	25.301686890502268
17	24.650521797369578	25.649382992440202	24.54363744694679	25.156457763243438
18	24.91222970685881	25.266004596860125	25.140276901623558	24.681488794657504
19	25.822454613366467	24.798804750099485	24.72917509634364	24.6495655401904
20	26.06957465038556	24.45862582695735	24.583807252338698	24.88799227031839
21	26.1870702156328	24.094605693394143	24.45636674821322	25.261957342759832
22	26.09992913449288	24.045247020788192	24.428722537528024	25.42610130719091
23	25.13354985214012	24.216914602828272	24.794096292835633	25.85543925219598
24	25.109171171754223	24.509040272820933	24.67710030678785	25.70468824863699
25	25.304098106372646	24.29465481268943	24.727279331850543	25.673967749087385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	556.0
1	556.0
2	941.5
3	1327.0
4	1327.0
5	1327.0
6	2689.0
7	4051.0
8	4051.0
9	4051.0
10	4738.0
11	5425.0
12	5425.0
13	5425.0
14	7338.0
15	9251.0
16	9251.0
17	9251.0
18	15823.5
19	22396.0
20	22396.0
21	22396.0
22	35636.0
23	48876.0
24	48876.0
25	48876.0
26	73951.0
27	99026.0
28	99026.0
29	99026.0
30	131906.0
31	164786.0
32	164786.0
33	164786.0
34	212565.0
35	260344.0
36	260344.0
37	260344.0
38	318195.0
39	376046.0
40	376046.0
41	376046.0
42	439762.5
43	503479.0
44	503479.0
45	503479.0
46	566057.0
47	628635.0
48	628635.0
49	628635.0
50	656748.0
51	684861.0
52	684861.0
53	684861.0
54	659784.0
55	634707.0
56	634707.0
57	634707.0
58	580439.0
59	526171.0
60	526171.0
61	526171.0
62	452346.5
63	378522.0
64	378522.0
65	378522.0
66	304405.5
67	230289.0
68	230289.0
69	230289.0
70	173829.5
71	117370.0
72	117370.0
73	117370.0
74	85800.0
75	54230.0
76	54230.0
77	54230.0
78	40209.0
79	26188.0
80	26188.0
81	26188.0
82	18132.0
83	10076.0
84	10076.0
85	10076.0
86	7035.5
87	3995.0
88	3995.0
89	3995.0
90	2749.5
91	1504.0
92	1504.0
93	1504.0
94	967.5
95	431.0
96	431.0
97	431.0
98	1076.5
99	1722.0
100	1722.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010637712065918773
2	3.3373214324451054E-4
3	6.674642864890211E-4
4	0.01113831028078554
5	0.03316463173492323
6	0.062032462125573395
7	0.10573051463165148
8	0.10848380481341871
9	0.11857920214656514
10	0.13215792872482618
11	0.13935402806353595
12	0.1316364722510066
13	0.1277568360857892
14	0.11513758941935613
15	0.13242908609121234
16	0.11868349344132906
17	0.12329316866989387
18	0.11144567758471374
19	0.09674060502300248
20	0.09211007153548491
21	0.09098372555203468
22	0.10241405145815917
23	0.08172265857699951
24	0.08987823782753723
25	0.08756297108377845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4794264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.00473611127341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50958719797703	31.73265365159778
2	14.823311717143916	13.342384642504182
3	5.629116187602739	7.600106656882762
4	2.6494624439984698	4.769534325155226
5	1.4844556877909385	3.340376824895502
6	0.9525806403415876	2.5722384205968596
7	0.652577304230365	2.0558348558365633
8	0.4654318827136449	1.6757311247440596
9	0.36484136949275414	1.4777630600847296
>10	2.2272677109644747	18.75410871677417
>50	0.164806593999761	5.07605876056167
>100	0.07194737049522391	5.6284020202575595
>500	0.003308954328410413	0.9803458203160677
>1k	0.0012583339592610002	0.8629304070723993
>5k	4.660496145405004E-5	0.1315307127204847
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6271	0.13080214189289535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.257477685834572E-5
2	0.0	0.0	0.0	0.0	6.257477685834572E-5
3	0.0	0.0	0.0	0.0	6.257477685834572E-5
4	0.0	0.0	0.0	0.0	6.257477685834572E-5
5	0.0	0.0	0.0	0.0	8.343303581112764E-5
6	0.0	0.0	0.0	0.0	1.4600781266947336E-4
7	0.0	0.0	0.0	0.0	1.4600781266947336E-4
8	0.0	0.0	0.0	0.0	1.4600781266947336E-4
9	0.0	0.0	0.0	2.085825895278191E-5	1.8772433057503716E-4
10	0.0	0.0	0.0	2.085825895278191E-5	1.8772433057503716E-4
11	0.0	0.0	0.0	2.085825895278191E-5	2.0858258952781907E-4
12	0.0	0.0	0.0	2.085825895278191E-5	3.9630692010285623E-4
13	0.0	0.0	0.0	2.085825895278191E-5	5.423147327723296E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4845	0.0	13.071775	1
GGTATCA	1645	0.0	13.04503	1
CGCGTAA	110	4.9649316E-7	11.22918	10
TTAGCCG	60	0.005876279	11.083942	4
GCGTTAT	190	0.0	10.994401	1
CGTTATT	195	1.8189894E-12	10.715515	2
GTATTAG	315	0.0	10.550183	1
CGAATTT	100	2.4022696E-5	10.449593	15
TAGACAG	520	0.0	10.41447	5
CGACCAT	340	0.0	10.339992	10
GTATAGT	230	0.0	10.320831	1
AAGACGG	565	0.0	10.089473	5
GTCTTAG	505	0.0	9.965222	1
GGACCGT	210	7.2759576E-12	9.953657	6
GTATAGG	440	0.0	9.710964	1
GGCGTAA	90	0.001113099	9.501218	6
TAGGCCG	90	0.0011134183	9.500919	5
CGAACCT	270	0.0	9.499431	16
CAAGACG	565	0.0	9.416448	4
GTATTAC	285	0.0	9.328584	1
>>END_MODULE