FastQCFastQC Report
Thu 2 Feb 2017
SRR4061984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794264
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48100.027.2697981
GGTATCA20300.024.7183531
TATCAAC74700.017.8175452
TCAACGC75500.017.2200954
ATCAACG77000.016.9417763
TATAACG1307.329636E-716.9227052
CAACGCA77450.016.8149385
AACGCAG80850.016.1894446
CGTCGTA5750.014.155766510
GTATAGG6850.014.136531
GTATATA6050.013.8232121
GTCCTAA6550.013.4400051
ACCGTCG5900.013.4228738
GTACATG148650.013.3099221
TATAGGG3650.013.2599822
CGCAATA4700.013.10636836
ATTAGAC3203.6379788E-1213.06166653
ACGCAGA100150.013.0475957
TACATGG153450.012.8742692
ATACGAA4450.012.85485640
Produced by FastQC (version 0.11.5)