##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4794264
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30467095679337	32.0	32.0	32.0	32.0	32.0
2	31.4471203504855	32.0	32.0	32.0	32.0	32.0
3	31.559869043506993	32.0	32.0	32.0	32.0	32.0
4	31.639685465798294	32.0	32.0	32.0	32.0	32.0
5	31.613032573925842	32.0	32.0	32.0	32.0	32.0
6	35.25214172602927	36.0	36.0	36.0	36.0	36.0
7	35.25534159153522	36.0	36.0	36.0	36.0	36.0
8	35.207925762953394	36.0	36.0	36.0	36.0	36.0
9	35.29250934032836	36.0	36.0	36.0	36.0	36.0
10	35.1710500297856	36.0	36.0	36.0	36.0	36.0
11	35.29446793084403	36.0	36.0	36.0	36.0	36.0
12	35.22298313150882	36.0	36.0	36.0	36.0	36.0
13	35.249582000490584	36.0	36.0	36.0	36.0	36.0
14	35.21505761885453	36.0	36.0	36.0	36.0	36.0
15	35.192861302589925	36.0	36.0	36.0	36.0	36.0
16	35.20791512524133	36.0	36.0	36.0	36.0	36.0
17	35.19105852326864	36.0	36.0	36.0	36.0	36.0
18	35.18435363592827	36.0	36.0	36.0	36.0	36.0
19	35.17666799325194	36.0	36.0	36.0	36.0	36.0
20	35.17673870274979	36.0	36.0	36.0	36.0	36.0
21	35.17215989774447	36.0	36.0	36.0	36.0	36.0
22	35.152543122364555	36.0	36.0	36.0	36.0	36.0
23	35.12139506710519	36.0	36.0	36.0	36.0	36.0
24	35.109930325071794	36.0	36.0	36.0	36.0	36.0
25	35.098657479020765	36.0	36.0	36.0	36.0	36.0
26	35.05775026156257	36.0	36.0	36.0	36.0	36.0
27	35.04072658493566	36.0	36.0	36.0	36.0	36.0
28	35.02544206994025	36.0	36.0	36.0	36.0	36.0
29	35.0064852498736	36.0	36.0	36.0	36.0	36.0
30	34.988597624160874	36.0	36.0	36.0	36.0	36.0
31	34.98392912864206	36.0	36.0	36.0	36.0	36.0
32	34.96192992292456	36.0	36.0	36.0	36.0	36.0
33	34.94458815784863	36.0	36.0	36.0	36.0	36.0
34	34.9354743501818	36.0	36.0	36.0	36.0	36.0
35	34.92075947423838	36.0	36.0	36.0	36.0	36.0
36	34.9039026636831	36.0	36.0	36.0	32.0	36.0
37	34.88991365515124	36.0	36.0	36.0	32.0	36.0
38	34.88336603908337	36.0	36.0	36.0	32.0	36.0
39	34.86504935898399	36.0	36.0	36.0	32.0	36.0
40	34.85648328919726	36.0	36.0	36.0	32.0	36.0
41	34.83398014794346	36.0	36.0	36.0	32.0	36.0
42	34.81297880133426	36.0	36.0	36.0	32.0	36.0
43	34.80094254300556	36.0	36.0	36.0	32.0	36.0
44	34.77614561901472	36.0	36.0	36.0	32.0	36.0
45	34.75600696999581	36.0	36.0	36.0	32.0	36.0
46	34.74254901273689	36.0	36.0	36.0	32.0	36.0
47	34.720144531047936	36.0	36.0	36.0	32.0	36.0
48	34.69669671924616	36.0	36.0	36.0	32.0	36.0
49	34.68258506415166	36.0	36.0	36.0	32.0	36.0
50	34.27322295977026	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	14.0
20	40.0
21	108.0
22	332.0
23	825.0
24	2144.0
25	5295.0
26	11173.0
27	20977.0
28	36577.0
29	58694.0
30	88186.0
31	130239.0
32	197666.0
33	336079.0
34	771986.0
35	3133928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99661574284654	17.36057152633753	12.222342773922442	26.420469956893495
2	16.388105043937962	19.79915862055155	36.61475351019768	27.197982825312806
3	18.96913723422362	23.33427751876412	27.755818629626788	29.94076661738547
4	13.410609011101599	15.80280101387825	34.2358284817023	36.55076149331784
5	15.422763535758566	35.89155707737413	32.567146907220796	16.11853247964651
6	35.31625566772224	33.51466150938185	16.712464546100396	14.456618276795513
7	31.267698232721436	29.489907105657924	19.916821434948094	19.32557322667254
8	28.60818678320593	32.56107715386554	18.803261564235928	20.027474498692605
9	27.387728335360755	14.95764521937048	18.157510725316754	39.497115719952006
10	16.81230604418365	26.614148329815933	30.507698578008462	26.06584704799195
11	36.759636098471006	21.274235211077237	21.972110839119413	19.99401785133234
12	25.296421994371187	22.901288477499044	28.041828326898212	23.760461201231557
13	29.710253753235115	19.565088614227335	24.822099909391724	25.902557723145826
14	24.054703704259925	18.81137542696856	24.819930650460634	32.313990218310884
15	25.47896820033273	26.5940298656895	21.826061309932037	26.100940624045734
16	26.16883114174235	25.491121209352734	23.036464219310112	25.303583429594802
17	24.836784123694482	25.538956553080933	24.52263788560663	25.101621437617954
18	25.085649315149638	25.115768672489324	25.129535137755386	24.669046874605648
19	26.008272804391414	24.618298996112646	24.77705123811522	24.596376961380717
20	26.297778859629528	24.28536773955629	24.540861352841706	24.875992047972478
21	26.36133076609721	23.98344604100611	24.43502762991001	25.220195562986664
22	26.250619490290898	23.942235971986527	24.44231273037947	25.36483180734311
23	25.2877137894662	24.02864591644051	24.79260098688828	25.89103930720501
24	25.30923708517218	24.39455193735454	24.67224060315989	25.623970374313394
25	25.56838364600283	24.007911138606993	24.83294060617994	25.590764609210236
26	25.31016914258968	24.421206368727134	24.91454848204272	25.35407600664047
27	25.544460530874442	24.50738292046645	24.507674937492713	25.440481611166398
28	25.545388632421368	24.273642546112008	24.620165717046195	25.560803104420426
29	25.475194837809674	24.56750219638383	24.574698325742286	25.38260464006421
30	25.521567292505697	24.570502214757653	24.56764459751817	25.34028589521848
31	25.66130850634459	24.125070787996858	24.46664699029774	25.74697371536081
32	25.615912124384494	24.435827143080925	24.33875213772553	25.609508594809054
33	25.409578756240542	24.365683510785306	24.615941371495676	25.608796361478475
34	25.72777573713073	24.27046172785583	24.67634538260672	25.325417152406715
35	25.774109126072403	24.334072482198998	24.654518649724995	25.237299742003604
36	25.407054902169367	24.532612470550692	24.605595655633667	25.454736971646273
37	25.92071359723198	24.223492231949972	24.308699552729358	25.54709461808869
38	25.561921496187946	24.41329889217615	24.505096089827344	25.519683521808563
39	25.81234981978374	24.387661026565212	24.294883026298226	25.50510612735282
40	25.883327145378015	24.359650915886917	24.456183018860052	25.30083891987502
41	25.471261068818208	24.49512547528676	24.617669101339093	25.41594435455594
42	25.81299871799204	24.46430381711299	24.39797148210216	25.324725982792806
43	25.680365052969893	24.221054291533413	24.432170418127157	25.666410237369536
44	25.467846046501442	24.443262422442842	24.468710660830258	25.620180870225457
45	25.548083958886615	24.520728987542334	24.360387938982377	25.570799114588677
46	25.655499967147566	24.326592847763898	24.360363066352537	25.657544118736002
47	25.646181440377052	24.458017317998422	24.546415744181047	25.34938549744348
48	25.738270236985727	24.78815380084121	24.124580416867264	25.3489955453058
49	25.669391429720473	24.493184893960354	24.151157182978256	25.68626649334092
50	25.53110229486586	24.807474896645516	24.263002223529362	25.398420584959265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	180.0
1	139.0
2	98.0
3	305.0
4	512.0
5	497.5
6	483.0
7	429.0
8	375.0
9	499.5
10	624.0
11	1036.0
12	1448.0
13	2593.0
14	3738.0
15	4746.5
16	5755.0
17	5435.5
18	5116.0
19	5028.5
20	4941.0
21	5938.0
22	6935.0
23	8612.5
24	10290.0
25	13457.5
26	16625.0
27	21414.5
28	26204.0
29	32750.0
30	39296.0
31	48612.5
32	57929.0
33	69457.0
34	80985.0
35	96455.5
36	111926.0
37	129347.0
38	146768.0
39	167033.5
40	187299.0
41	208297.5
42	229296.0
43	246366.0
44	263436.0
45	287809.5
46	312183.0
47	332301.0
48	352419.0
49	369023.5
50	385628.0
51	393798.0
52	401968.0
53	402856.5
54	403745.0
55	393147.5
56	382550.0
57	366488.5
58	350427.0
59	325412.0
60	300397.0
61	264231.5
62	228066.0
63	195866.0
64	163666.0
65	136416.5
66	109167.0
67	91920.0
68	74673.0
69	62725.0
70	50777.0
71	39988.0
72	29199.0
73	24648.5
74	20098.0
75	15835.0
76	11572.0
77	9439.0
78	7306.0
79	6047.0
80	4788.0
81	3717.5
82	2647.0
83	2092.5
84	1538.0
85	1119.5
86	701.0
87	467.0
88	233.0
89	158.0
90	83.0
91	66.0
92	49.0
93	45.0
94	41.0
95	36.0
96	31.0
97	33.0
98	35.0
99	26.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03095365628592835
2	0.004839116077045403
3	1.6686607162225527E-4
4	0.0
5	0.0
6	2.0858258952781907E-4
7	0.0
8	0.0
9	0.0
10	4.171651790556382E-5
11	0.0
12	2.085825895278191E-5
13	0.0
14	0.0
15	0.0
16	6.257477685834573E-4
17	0.0
18	1.0429129476390954E-4
19	2.085825895278191E-5
20	3.128738842917286E-4
21	6.257477685834572E-5
22	0.0
23	6.257477685834572E-5
24	9.594799118279678E-4
25	2.2944084848060096E-4
26	5.214564738195477E-4
27	4.797399559139839E-4
28	0.0014183616087891698
29	4.1716517905563815E-4
30	0.0012514955371669145
31	3.9630692010285623E-4
32	6.883225454418029E-4
33	1.8772433057503719E-4
34	6.883225454418029E-4
35	2.2944084848060096E-4
36	1.8772433057503719E-4
37	0.001564369421458643
38	0.0
39	5.005982148667658E-4
40	8.343303581112764E-5
41	0.001105487724497441
42	0.004630533487517583
43	0.00458881696961202
44	0.0045679587106592385
45	0.0022526919669004462
46	0.002064967636325409
47	0.0012723537961196964
48	0.00458881696961202
49	0.004338517862178637
50	0.0017520937520336803
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4794264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.797457664714806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.39485234056674	31.781078122162405
2	15.756081002014986	14.43176905303197
3	5.774239225372095	7.933364294097424
4	2.687927122450992	4.924009143851521
5	1.5460075089380512	3.5401606719960803
6	0.9776783635682864	2.6865110079135754
7	0.6675355624380196	2.1400002162312757
8	0.47656363771585164	1.7460322418267362
9	0.345932821915143	1.425855938984468
>10	2.147323299363454	18.300121186265127
>50	0.1599513054070293	4.994717337619101
>100	0.06269313316586589	4.909502868598812
>500	0.002525818083578096	0.7739778127606042
>1k	6.888589998326903E-4	0.41290010466071436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.343303581112764E-5
2	0.0	0.0	0.0	0.0	8.343303581112764E-5
3	0.0	0.0	0.0	0.0	8.343303581112764E-5
4	0.0	0.0	0.0	0.0	8.343303581112764E-5
5	0.0	0.0	0.0	0.0	8.343303581112764E-5
6	0.0	0.0	0.0	0.0	1.0429129476390954E-4
7	0.0	0.0	0.0	0.0	1.0429129476390954E-4
8	0.0	0.0	0.0	0.0	1.0429129476390954E-4
9	0.0	0.0	0.0	0.0	1.2514955371669144E-4
10	0.0	0.0	0.0	0.0	1.2514955371669144E-4
11	0.0	0.0	0.0	0.0	1.4600781266947336E-4
12	0.0	0.0	0.0	0.0	3.128738842917286E-4
13	0.0	0.0	0.0	0.0	3.9630692010285623E-4
14	0.0	0.0	0.0	2.085825895278191E-5	3.9630692010285623E-4
15	0.0	0.0	0.0	4.171651790556382E-5	6.257477685834573E-4
16	0.0	0.0	0.0	4.171651790556382E-5	6.883225454418029E-4
17	0.0	0.0	0.0	4.171651790556382E-5	6.883225454418029E-4
18	0.0	2.085825895278191E-5	0.0	4.171651790556382E-5	8.551886170640583E-4
19	0.0	2.085825895278191E-5	0.0	4.171651790556382E-5	8.551886170640583E-4
20	0.0	6.257477685834572E-5	0.0	4.171651790556382E-5	9.386216528751858E-4
21	0.0	6.257477685834572E-5	0.0	4.171651790556382E-5	0.0010429129476390955
22	0.0	6.257477685834572E-5	0.0	6.257477685834572E-5	0.0010846294655446592
23	0.0	6.257477685834572E-5	0.0	1.4600781266947336E-4	0.001147204242403005
24	0.0	6.257477685834572E-5	0.0	3.9630692010285623E-4	0.0011680625013557868
25	0.0	6.257477685834572E-5	0.0	4.797399559139839E-4	0.0011680625013557868
26	0.0	6.257477685834572E-5	0.0	6.674642864890211E-4	0.0012097790192613506
27	0.0	6.257477685834572E-5	0.0	8.551886170640583E-4	0.0012514955371669145
28	0.0	6.257477685834572E-5	0.0	0.0010429129476390955	0.0012514955371669145
29	0.0	6.257477685834572E-5	0.0	0.0013975033498363877	0.0012514955371669145
30	0.0	6.257477685834572E-5	0.0	0.0023569832616643555	0.0012514955371669145
31	0.0	6.257477685834572E-5	0.0	0.003942210942075781	0.0012514955371669145
32	0.0	6.257477685834572E-5	0.0	0.005756879470967807	0.0013349285729780422
33	0.0	6.257477685834572E-5	0.0	0.007404681928237577	0.0013349285729780422
34	0.0	6.257477685834572E-5	0.0	0.009219350457129603	0.0014183616087891698
35	0.0	6.257477685834572E-5	0.0	0.01180577456727456	0.0014183616087891698
36	0.0	6.257477685834572E-5	0.0	0.014955371669144627	0.0014183616087891698
37	0.0	6.257477685834572E-5	0.0	0.020378518996867923	0.0014183616087891698
38	0.0	6.257477685834572E-5	0.0	0.027595476594530464	0.0014392198677419517
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4810	0.0	27.269798	1
GGTATCA	2030	0.0	24.718353	1
TATCAAC	7470	0.0	17.817545	2
TCAACGC	7550	0.0	17.220095	4
ATCAACG	7700	0.0	16.941776	3
TATAACG	130	7.329636E-7	16.922705	2
CAACGCA	7745	0.0	16.814938	5
AACGCAG	8085	0.0	16.189444	6
CGTCGTA	575	0.0	14.1557665	10
GTATAGG	685	0.0	14.13653	1
GTATATA	605	0.0	13.823212	1
GTCCTAA	655	0.0	13.440005	1
ACCGTCG	590	0.0	13.422873	8
GTACATG	14865	0.0	13.309922	1
TATAGGG	365	0.0	13.259982	2
CGCAATA	470	0.0	13.106368	36
ATTAGAC	320	3.6379788E-12	13.0616665	3
ACGCAGA	10015	0.0	13.047595	7
TACATGG	15345	0.0	12.874269	2
ATACGAA	445	0.0	12.854856	40
>>END_MODULE