FastQCFastQC Report
Thu 2 Feb 2017
SRR4061983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886954
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13520.1524318059335659No Hit
TATCAACGCAGAGTACTTTTTTTTT9600.10823560184631897No Hit
GCGCAAGACGGACCAGAGCGAAAGC8960.10101989505656436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA1450.015.07183210
GAATTTC1550.014.708323518
TGCACCG400.005276003414.2502975
CCCCTAT400.005283911714.2470811
TCTAGGA852.7013266E-713.4090172
CCGGTCC1850.013.353899
CCGATAA500.001496513713.3025299
TTGTATC500.001498424913.3002774
GTCTAGG655.4578166E-513.151151
CGCCGGT1600.013.0649847
TCGCGTA801.9923755E-613.0649849
GGTCCAA1850.012.8388311
ATCACGA751.4765683E-512.6669312
TACACTA604.0882247E-412.6669295
TCACGAA905.398506E-712.66621513
GTATAAG751.48E-512.6640711
CAAGACG1900.012.500264
GGTATCA3800.012.4974391
CGACCAT1600.012.47112210
CCGACCA1700.012.2964559
Produced by FastQC (version 0.11.5)