##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061983_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	886954
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250865321087677	32.0	32.0	32.0	32.0	32.0
2	30.75433900743443	32.0	32.0	32.0	32.0	32.0
3	30.794519219711507	32.0	32.0	32.0	32.0	32.0
4	30.796271283516393	32.0	32.0	32.0	32.0	32.0
5	30.6997634601118	32.0	32.0	32.0	32.0	32.0
6	34.36229387318846	36.0	36.0	36.0	32.0	36.0
7	34.29801658259617	36.0	36.0	36.0	32.0	36.0
8	34.249631886208306	36.0	36.0	36.0	32.0	36.0
9	34.39214096785177	36.0	36.0	36.0	32.0	36.0
10	34.055026528997	36.0	36.0	36.0	32.0	36.0
11	34.377488573251824	36.0	36.0	36.0	32.0	36.0
12	34.18118865239911	36.0	36.0	36.0	32.0	36.0
13	34.27891299887029	36.0	36.0	36.0	32.0	36.0
14	34.18589577362524	36.0	36.0	36.0	32.0	36.0
15	34.129741790442345	36.0	36.0	36.0	32.0	36.0
16	34.126121535051425	36.0	36.0	36.0	32.0	36.0
17	34.05498819555467	36.0	36.0	36.0	32.0	36.0
18	34.06256017786717	36.0	36.0	36.0	32.0	36.0
19	34.060917477118316	36.0	36.0	36.0	32.0	36.0
20	34.04358399646431	36.0	36.0	36.0	32.0	36.0
21	34.031877639652116	36.0	36.0	36.0	32.0	36.0
22	33.99307179402765	36.0	36.0	36.0	32.0	36.0
23	33.95295471918499	36.0	36.0	36.0	32.0	36.0
24	33.919458055321925	36.0	36.0	36.0	32.0	36.0
25	33.56820421352178	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	22.0
5	66.0
6	195.0
7	49.0
8	168.0
9	113.0
10	66.0
11	16.0
12	38.0
13	42.0
14	136.0
15	213.0
16	331.0
17	443.0
18	582.0
19	844.0
20	1259.0
21	1792.0
22	2725.0
23	3996.0
24	5550.0
25	7717.0
26	10645.0
27	13908.0
28	17962.0
29	24063.0
30	31034.0
31	42619.0
32	59743.0
33	84187.0
34	183136.0
35	393294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.17502717182969	17.385573900611988	11.905883997708997	25.53351492984933
2	16.678880753680573	18.95442153708084	38.13906921892229	26.227628490316295
3	19.95882500174757	22.204170265157067	27.818717261253784	30.018287471841575
4	12.935994885273852	15.401572530222102	34.76694506743523	36.89548751706882
5	15.005368606733766	36.336895862563665	33.259421002530914	15.39831452817165
6	35.53204139585206	34.23314669172771	16.326157616071864	13.908654296348361
7	31.17392045345526	29.85213268216484	19.336521455490256	19.637425408889648
8	28.215634153361663	33.68098471124054	18.46006241922467	19.643318716173123
9	26.65722968000036	15.085294104366751	18.498977283502434	39.75849893213045
10	16.968589898460767	26.661104625826688	30.520701855794876	25.849603619917676
11	37.33950692553302	21.289634958699708	21.479539532216034	19.891318583551236
12	24.602292035228384	23.564429762814658	28.350585879066976	23.482692322889985
13	29.737687003562836	19.46440102414508	24.766993447819626	26.03091852447246
14	23.301629514577137	19.845853068264425	24.742636932954664	32.10988048420378
15	25.147695520871554	27.392848070898367	21.729220174423528	25.730236233806554
16	25.718456005364292	25.981703613897487	23.700525366367973	24.599315014370248
17	24.06026665522773	26.31817786723229	24.826186441921607	24.795369035618375
18	24.631893565460018	25.485099096678944	25.91988514063387	23.963122197227165
19	25.579663121067632	25.149789803346216	24.917980983551054	24.3525660920351
20	25.65190306264093	24.84397888269695	24.807188369683715	24.69692968497841
21	26.403219417620356	24.47572176774556	24.403384180300026	24.71767463433406
22	25.77982334763677	24.654584361322478	24.920939828494166	24.644652462546585
23	24.51011921889899	24.72181316542257	25.37889943973324	25.389168175945205
24	24.73561319344052	25.054649156365993	25.283966834705062	24.925770815488423
25	24.78274067796419	24.943660970509608	25.1154146067327	25.158183744793504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	465.0
1	465.0
2	392.0
3	319.0
4	319.0
5	319.0
6	575.5
7	832.0
8	832.0
9	832.0
10	1022.0
11	1212.0
12	1212.0
13	1212.0
14	1669.5
15	2127.0
16	2127.0
17	2127.0
18	3586.5
19	5046.0
20	5046.0
21	5046.0
22	8316.5
23	11587.0
24	11587.0
25	11587.0
26	17644.5
27	23702.0
28	23702.0
29	23702.0
30	30656.0
31	37610.0
32	37610.0
33	37610.0
34	45426.0
35	53242.0
36	53242.0
37	53242.0
38	62206.5
39	71171.0
40	71171.0
41	71171.0
42	81392.5
43	91614.0
44	91614.0
45	91614.0
46	102513.5
47	113413.0
48	113413.0
49	113413.0
50	116988.0
51	120563.0
52	120563.0
53	120563.0
54	113938.0
55	107313.0
56	107313.0
57	107313.0
58	99014.5
59	90716.0
60	90716.0
61	90716.0
62	79873.0
63	69030.0
64	69030.0
65	69030.0
66	56022.0
67	43014.0
68	43014.0
69	43014.0
70	32596.5
71	22179.0
72	22179.0
73	22179.0
74	16686.5
75	11194.0
76	11194.0
77	11194.0
78	8786.5
79	6379.0
80	6379.0
81	6379.0
82	4350.5
83	2322.0
84	2322.0
85	2322.0
86	1711.0
87	1100.0
88	1100.0
89	1100.0
90	740.0
91	380.0
92	380.0
93	380.0
94	234.5
95	89.0
96	89.0
97	89.0
98	212.0
99	335.0
100	335.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.764725115394936E-4
2	0.0
3	9.019633487193247E-4
4	0.011612778114761307
5	0.03585304311159316
6	0.06595606987510062
7	0.10789736559054923
8	0.11376012735722485
9	0.12153956123992901
10	0.13394155728481974
11	0.14048079156303483
12	0.1342797935405895
13	0.12875526802968362
14	0.11793170784505172
15	0.1329268485175105
16	0.12356897877454749
17	0.12300525168159793
18	0.11285816400850551
19	0.09910322294053582
20	0.09651007831296775
21	0.09369144284821987
22	0.10372578510272235
23	0.08670122689564509
24	0.09549536954565851
25	0.0903090802905224
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	886954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.2014291236672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76788658017743	46.8173791205394
2	13.9451974518463	16.790416320257386
3	4.065239176854883	7.341996245285531
4	1.6023095511733143	3.8584529951654103
5	0.7734469992027657	2.328130735170918
6	0.44117523131084424	1.5935627651326363
7	0.2668503264683661	1.1245339710858955
8	0.18700897892497412	0.900656623219296
9	0.13435360202492208	0.7279450964831461
>10	0.6914623147875292	7.663560556334679
>50	0.06829103061692897	2.930610537211251
>100	0.05281744807468726	6.241836847055645
>500	0.0037726747972186042	1.5273847869166737
>1k	1.886337398609302E-4	0.15353340014210726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1352	0.1524318059335659	No Hit
TATCAACGCAGAGTACTTTTTTTTT	960	0.10823560184631897	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	896	0.10101989505656436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	4.509816743596624E-4
13	0.0	0.0	0.0	1.127454185899156E-4	5.63727092949578E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	145	0.0	15.071832	10
GAATTTC	155	0.0	14.7083235	18
TGCACCG	40	0.0052760034	14.250297	5
CCCCTAT	40	0.0052839117	14.247081	1
TCTAGGA	85	2.7013266E-7	13.409017	2
CCGGTCC	185	0.0	13.35389	9
CCGATAA	50	0.0014965137	13.302529	9
TTGTATC	50	0.0014984249	13.300277	4
GTCTAGG	65	5.4578166E-5	13.15115	1
CGCCGGT	160	0.0	13.064984	7
TCGCGTA	80	1.9923755E-6	13.064984	9
GGTCCAA	185	0.0	12.83883	11
ATCACGA	75	1.4765683E-5	12.66693	12
TACACTA	60	4.0882247E-4	12.666929	5
TCACGAA	90	5.398506E-7	12.666215	13
GTATAAG	75	1.48E-5	12.664071	1
CAAGACG	190	0.0	12.50026	4
GGTATCA	380	0.0	12.497439	1
CGACCAT	160	0.0	12.471122	10
CCGACCA	170	0.0	12.296455	9
>>END_MODULE