FastQCFastQC Report
Thu 2 Feb 2017
SRR4061983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886954
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9120.10282382175400301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.026.3199351
GTATCAA11350.024.626711
TATACCC703.2138552E-522.0002254
AACGTAT703.2152544E-521.99898521
CAACGTA803.6151941E-621.99898320
GTCGATA550.004480958520.00133341
GTAGGAC3300.019.9990773
GCCGGTT1001.1232423E-619.79908811
GTGTAGA2400.019.25781
GTATTAC1152.011293E-719.1381821
TGTAGGA3950.018.9358352
GTGTAAG1404.0563464E-918.864781
GTAATAC1051.7923812E-618.8562743
TAGGACG2250.018.5779694
GTCCTAC3800.018.5338211
GGCGTAC600.00739329418.3407611
GCGTACT600.00741255618.3324872
ATTACAC1456.3027983E-918.2060593
GTACCGT851.4301768E-418.1178326
CGTCGTA1701.2551027E-1018.11783210
Produced by FastQC (version 0.11.5)