##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	886954
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263116238271657	32.0	32.0	32.0	32.0	32.0
2	31.441651990971348	32.0	32.0	32.0	32.0	32.0
3	31.575835950906136	32.0	32.0	32.0	32.0	32.0
4	31.658881971331095	32.0	32.0	32.0	32.0	32.0
5	31.630496057292714	32.0	32.0	32.0	32.0	32.0
6	35.287709396428674	36.0	36.0	36.0	36.0	36.0
7	35.29509083898376	36.0	36.0	36.0	36.0	36.0
8	35.236005474917526	36.0	36.0	36.0	36.0	36.0
9	35.32224444559695	36.0	36.0	36.0	36.0	36.0
10	35.2045201893221	36.0	36.0	36.0	36.0	36.0
11	35.32071449026669	36.0	36.0	36.0	36.0	36.0
12	35.247910263666434	36.0	36.0	36.0	36.0	36.0
13	35.28090070060003	36.0	36.0	36.0	36.0	36.0
14	35.241878383771876	36.0	36.0	36.0	36.0	36.0
15	35.22505000259314	36.0	36.0	36.0	36.0	36.0
16	35.238837639832504	36.0	36.0	36.0	36.0	36.0
17	35.21448801177964	36.0	36.0	36.0	36.0	36.0
18	35.21261869273942	36.0	36.0	36.0	36.0	36.0
19	35.200066745287806	36.0	36.0	36.0	36.0	36.0
20	35.200846943584445	36.0	36.0	36.0	36.0	36.0
21	35.184216994342435	36.0	36.0	36.0	36.0	36.0
22	35.17844330145645	36.0	36.0	36.0	36.0	36.0
23	35.13332822220769	36.0	36.0	36.0	36.0	36.0
24	35.126668350331585	36.0	36.0	36.0	36.0	36.0
25	35.1034878922695	36.0	36.0	36.0	36.0	36.0
26	35.05892639302602	36.0	36.0	36.0	36.0	36.0
27	35.05044906500224	36.0	36.0	36.0	36.0	36.0
28	35.027444489793155	36.0	36.0	36.0	36.0	36.0
29	35.00421780610945	36.0	36.0	36.0	36.0	36.0
30	34.982609019182505	36.0	36.0	36.0	36.0	36.0
31	34.98383794424514	36.0	36.0	36.0	36.0	36.0
32	34.95420168351459	36.0	36.0	36.0	36.0	36.0
33	34.94558342371758	36.0	36.0	36.0	36.0	36.0
34	34.93027485078144	36.0	36.0	36.0	36.0	36.0
35	34.90279540990852	36.0	36.0	36.0	36.0	36.0
36	34.88840571213389	36.0	36.0	36.0	32.0	36.0
37	34.87327527695912	36.0	36.0	36.0	32.0	36.0
38	34.848942560719046	36.0	36.0	36.0	32.0	36.0
39	34.8240720488323	36.0	36.0	36.0	32.0	36.0
40	34.8213853255073	36.0	36.0	36.0	32.0	36.0
41	34.79783957228898	36.0	36.0	36.0	32.0	36.0
42	34.7624972659236	36.0	36.0	36.0	32.0	36.0
43	34.76577703015038	36.0	36.0	36.0	32.0	36.0
44	34.711971534036714	36.0	36.0	36.0	32.0	36.0
45	34.69558736980723	36.0	36.0	36.0	32.0	36.0
46	34.67229191141818	36.0	36.0	36.0	32.0	36.0
47	34.642468493292775	36.0	36.0	36.0	32.0	36.0
48	34.609333742223384	36.0	36.0	36.0	32.0	36.0
49	34.59365536431427	36.0	36.0	36.0	32.0	36.0
50	34.18254159742219	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	20.0
22	71.0
23	192.0
24	412.0
25	982.0
26	2086.0
27	4096.0
28	6876.0
29	10812.0
30	16096.0
31	23730.0
32	36563.0
33	63244.0
34	144951.0
35	576812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93375324946289	17.998500031014398	12.261062271268827	25.80668444825389
2	16.18552377000753	19.460918508629906	37.77426498112561	26.579292740236955
3	19.20207542468524	22.680170606944465	28.229518879847927	29.888235088522364
4	12.767643832311295	15.898023908820422	34.82879025156914	36.50554200729915
5	14.684430854848001	36.680861330870975	33.07300386829967	15.561703945981353
6	35.61932185885627	34.558049233669394	16.0938447766175	13.728784130856841
7	31.300495854350956	29.847432899564126	19.322760819614096	19.52931042647082
8	28.17023205262054	33.951366136237056	18.34525804043953	19.53314377070288
9	26.763056483199804	14.762659619326367	18.493067284210905	39.98121661326292
10	16.931149522352417	26.766642125664212	30.580043316936333	25.722165035047034
11	37.52821454100213	21.310462549354305	21.47777675054174	19.683546159101827
12	24.65539362807992	23.49467954369674	28.421879826913234	23.4280470013101
13	29.911697788160378	19.29976075422175	24.83386962570776	25.954671831910108
14	23.367277220690138	19.70575700656404	24.795310692550007	32.13165508019581
15	25.142566581806946	27.41359754846362	21.777341327735147	25.666494541994282
16	25.908846967353217	25.954509170774386	23.542079129779875	24.594564732092525
17	24.04668111311297	26.37216811694857	24.785050859458327	24.796099910480137
18	24.76202180278279	25.352133319653507	25.972235219307493	23.913609658256206
19	25.76480854700469	25.00817404284777	24.94176699129831	24.28525041884923
20	25.89923028615947	24.595215304531358	24.834461352518115	24.671093056791058
21	26.545683316158446	24.23507870757672	24.58797186776315	24.63126610850168
22	25.844857794203534	24.51231969188932	25.004453444034304	24.638369069872844
23	24.581658124322118	24.493716697821984	25.411802641399667	25.51282253645623
24	24.87609138795685	24.863801998773315	25.37420626330411	24.885900349965727
25	24.999492645616346	24.603643480947152	25.415635985631724	24.98122788780478
26	24.5121494737032	25.652459208615575	25.519081077668236	24.31631024001299
27	25.058458763177182	24.961948249619482	25.129150459439654	24.850442527763686
28	24.772221702330604	24.950926615982873	25.36888189352795	24.907969788158574
29	24.553103835499368	24.987626148456904	25.247843454711706	25.211426561332022
30	24.72740638349928	25.128672304759153	25.45958421228873	24.68433709945284
31	25.24189638649304	24.772196854388636	24.872653475393204	25.113253283725122
32	24.968853902535546	25.01823667426199	24.832882161206562	25.180027261995896
33	24.68205720264456	24.742939865967944	25.30993785458514	25.26506507680235
34	25.23270811817604	25.003382385438606	25.326061956281542	24.437847540103817
35	25.45690080883563	24.76689884706535	25.427925236258027	24.348275107840994
36	24.476945227086443	25.26436011829263	25.254438510270553	25.00425614435038
37	25.321412263443616	25.035825220278596	24.91022554949856	24.732536966779225
38	24.904561003163636	24.777496916412804	25.212355995914105	25.105586084509458
39	25.399318337356487	24.72656263212428	24.870764835407673	25.003354195111555
40	25.653783233158915	24.9478833714977	25.07404563714199	24.324287758201393
41	24.637293899051123	25.122668398455822	25.77829039939387	24.461747303099184
42	25.578998758623957	25.289344348243752	25.045691883926267	24.085965009206024
43	25.10496953515747	24.69174359922609	25.3275365194763	24.875750346140144
44	24.592971600467237	25.19426867933378	25.033261323507528	25.179498396691457
45	24.70789986684541	25.418997247837787	25.052681187770524	24.82042169754628
46	24.725883683356614	25.042928447326258	24.961299567840474	25.26988830147665
47	24.954647490644813	25.006849384570113	25.18262206843522	24.85588105634986
48	25.442038348701228	25.33052810080797	24.476555787821592	24.75087776266921
49	24.945964504006575	25.387381231840184	24.508945030932995	25.157709233220242
50	24.96349805341743	25.655428389100038	24.53336700720117	24.847706550281362
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	139.5
2	30.0
3	69.0
4	108.0
5	120.5
6	133.0
7	105.0
8	77.0
9	110.0
10	143.0
11	263.0
12	383.0
13	676.5
14	970.0
15	1221.5
16	1473.0
17	1544.5
18	1616.0
19	1592.5
20	1569.0
21	1855.5
22	2142.0
23	2576.0
24	3010.0
25	3921.0
26	4832.0
27	6458.5
28	8085.0
29	9801.5
30	11518.0
31	13108.0
32	14698.0
33	17165.0
34	19632.0
35	22669.0
36	25706.0
37	29472.5
38	33239.0
39	35936.5
40	38634.0
41	41393.0
42	44152.0
43	45990.5
44	47829.0
45	51973.0
46	56117.0
47	59971.5
48	63826.0
49	66471.0
50	69116.0
51	67719.5
52	66323.0
53	66478.5
54	66634.0
55	66528.5
56	66423.0
57	63680.5
58	60938.0
59	56424.5
60	51911.0
61	45111.0
62	38311.0
63	33213.0
64	28115.0
65	23653.0
66	19191.0
67	16563.0
68	13935.0
69	12207.0
70	10479.0
71	8029.5
72	5580.0
73	4921.5
74	4263.0
75	3059.0
76	1855.0
77	1644.5
78	1434.0
79	1215.5
80	997.0
81	787.0
82	577.0
83	461.5
84	346.0
85	284.5
86	223.0
87	145.5
88	68.0
89	45.0
90	22.0
91	15.5
92	9.0
93	7.5
94	6.0
95	8.0
96	10.0
97	11.0
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030328517600687294
2	0.004284325906416793
3	3.382362557697468E-4
4	1.1274541858991558E-4
5	1.1274541858991558E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	3.382362557697468E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.764725115394936E-4
17	0.0
18	3.382362557697468E-4
19	0.0
20	1.1274541858991558E-4
21	0.0
22	0.0
23	0.0
24	0.001127454185899156
25	0.0
26	2.2549083717983117E-4
27	4.5098167435966233E-4
28	0.0019166721160285651
29	3.382362557697468E-4
30	0.0012401996044890716
31	4.5098167435966233E-4
32	5.63727092949578E-4
33	2.2549083717983117E-4
34	6.764725115394936E-4
35	0.0
36	1.1274541858991558E-4
37	0.0010147087673092403
38	0.0
39	5.63727092949578E-4
40	1.1274541858991558E-4
41	0.001127454185899156
42	0.003946089650647046
43	0.003382362557697468
44	0.004284325906416793
45	0.001691181278848734
46	0.0014656904416689028
47	0.0014656904416689028
48	0.004509816743596624
49	0.004171580487826877
50	0.001691181278848734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	886954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.801017308209914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44915437030323	47.86436529745332
2	14.300924845373228	17.676234077846402
3	4.138757244606093	7.6733822432514085
4	1.622889350897859	4.0118485145659255
5	0.7618311322907656	2.3540969496317383
6	0.42738852578758657	1.5847827407717376
7	0.26006007273408804	1.1250383939349593
8	0.18207048313437305	0.9001712863601223
9	0.12699837797025443	0.706376605954885
>10	0.625878990530544	7.0676767310557675
>50	0.05533504554483065	2.393555066564384
>100	0.04577048553610543	5.424443209548678
>500	0.002941075290992088	1.218028883060653
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	912	0.10282382175400301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.254908371798312E-4
2	0.0	0.0	0.0	0.0	2.254908371798312E-4
3	0.0	0.0	0.0	0.0	2.254908371798312E-4
4	0.0	0.0	0.0	0.0	2.254908371798312E-4
5	0.0	0.0	0.0	0.0	2.254908371798312E-4
6	0.0	0.0	0.0	0.0	2.254908371798312E-4
7	0.0	0.0	0.0	1.127454185899156E-4	2.254908371798312E-4
8	1.127454185899156E-4	0.0	0.0	1.127454185899156E-4	2.254908371798312E-4
9	1.127454185899156E-4	0.0	0.0	1.127454185899156E-4	2.254908371798312E-4
10	1.127454185899156E-4	0.0	0.0	1.127454185899156E-4	2.254908371798312E-4
11	1.127454185899156E-4	0.0	0.0	1.127454185899156E-4	2.254908371798312E-4
12	1.127454185899156E-4	0.0	0.0	1.127454185899156E-4	3.382362557697468E-4
13	1.127454185899156E-4	0.0	0.0	2.254908371798312E-4	4.509816743596624E-4
14	1.127454185899156E-4	0.0	0.0	2.254908371798312E-4	4.509816743596624E-4
15	1.127454185899156E-4	0.0	0.0	7.892179301294092E-4	6.764725115394936E-4
16	1.127454185899156E-4	0.0	0.0	0.001127454185899156	7.892179301294092E-4
17	1.127454185899156E-4	0.0	0.0	0.0013529450230789872	7.892179301294092E-4
18	1.127454185899156E-4	0.0	0.0	0.0018039266974386496	9.019633487193248E-4
19	1.127454185899156E-4	0.0	0.0	0.002029417534618481	9.019633487193248E-4
20	1.127454185899156E-4	0.0	0.0	0.0025931446275680586	0.0010147087673092405
21	1.127454185899156E-4	0.0	0.0	0.0025931446275680586	0.0013529450230789872
22	1.127454185899156E-4	0.0	0.0	0.003382362557697468	0.0014656904416689028
23	1.127454185899156E-4	0.0	0.0	0.0049607984179562865	0.0014656904416689028
24	1.127454185899156E-4	0.0	0.0	0.006539234278215105	0.0014656904416689028
25	1.127454185899156E-4	0.0	0.0	0.00845590639424367	0.0014656904416689028
26	1.127454185899156E-4	0.0	0.0	0.009357869742962995	0.0014656904416689028
27	1.127454185899156E-4	0.0	0.0	0.010823560184631898	0.0015784358602588184
28	1.127454185899156E-4	0.0	0.0	0.011387287277581476	0.001691181278848734
29	1.127454185899156E-4	0.0	0.0	0.012852977719250378	0.001691181278848734
30	1.127454185899156E-4	0.0	0.0	0.01668632195130751	0.001691181278848734
31	1.127454185899156E-4	0.0	0.0	0.022098102043623457	0.0018039266974386496
32	1.127454185899156E-4	0.0	0.0	0.027735372973119236	0.0018039266974386496
33	1.127454185899156E-4	0.0	0.0	0.03337264390261502	0.0018039266974386496
34	1.127454185899156E-4	0.0	0.0	0.03991187818083012	0.0019166721160285651
35	1.127454185899156E-4	0.0	0.0	0.046225621621865394	0.0019166721160285651
36	1.127454185899156E-4	0.0	0.0	0.05456878259751915	0.0019166721160285651
37	1.127454185899156E-4	0.0	0.0	0.06888745075843843	0.0019166721160285651
38	1.127454185899156E-4	0.0	0.0	0.08703946315141484	0.002029417534618481
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	485	0.0	26.319935	1
GTATCAA	1135	0.0	24.62671	1
TATACCC	70	3.2138552E-5	22.000225	4
AACGTAT	70	3.2152544E-5	21.998985	21
CAACGTA	80	3.6151941E-6	21.998983	20
GTCGATA	55	0.0044809585	20.001333	41
GTAGGAC	330	0.0	19.999077	3
GCCGGTT	100	1.1232423E-6	19.799088	11
GTGTAGA	240	0.0	19.2578	1
GTATTAC	115	2.011293E-7	19.138182	1
TGTAGGA	395	0.0	18.935835	2
GTGTAAG	140	4.0563464E-9	18.86478	1
GTAATAC	105	1.7923812E-6	18.856274	3
TAGGACG	225	0.0	18.577969	4
GTCCTAC	380	0.0	18.533821	1
GGCGTAC	60	0.007393294	18.340761	1
GCGTACT	60	0.007412556	18.332487	2
ATTACAC	145	6.3027983E-9	18.206059	3
GTACCGT	85	1.4301768E-4	18.117832	6
CGTCGTA	170	1.2551027E-10	18.117832	10
>>END_MODULE