FastQCFastQC Report
Thu 2 Feb 2017
SRR4061982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722549
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGAC32460.4492428887175818No Hit
ATGTAGGCAAGGGAAGTCGGCAAAA25370.35111805566127696No Hit
GTATCAACGCAGAGTACATGGGCAG24670.3414301313820931No Hit
GTCGGTGGACTGCTCGAGCTGCTTC23350.3231614741699179No Hit
TTCCCAATCCGTCCCCCGACCGGCA22970.31790231527550383No Hit
GTTCTGAGTTGACTGTTCGACGCCC20720.2867625586638415No Hit
GTATAAGGGAGCTTGACTGCGAGAG19740.2731994646729841No Hit
CCCTTATACCTTTACACTCTGCGAA19690.2725074700816138No Hit
CCCCTTGTCCGTACCAGTTCTGAGT19600.27126187981714733No Hit
CCTTTACACTCTGCGAATGATTTCC18590.2572835890714678No Hit
TCCCTACATAGCTACCCAGCGATGC17860.24718046803746183No Hit
TATCAACGCAGAGTACATGGGCAGA17460.24164451130649964No Hit
GTATCAACGCAGAGTACTTTTTTTT16900.23389417188315256No Hit
GTACATGGGCAGAAATCACATTGCG16740.23167978919076768No Hit
GTCCACCGACAGCCGACGGGTTCGG16600.22974220433493092No Hit
ACGCAGAGTACATGGGCAGAAATCA16060.22226866274813198No Hit
GTCCCGAACCCGTCGGCTGTCGGTG15820.21894708870955465No Hit
TCGCAATGCTTTGTTTTAATTAAAC15380.21285753630549625No Hit
GTCCTAAGGTAGCGAAATTCCTTGT15320.21202714279585194No Hit
GTCTCACGACGTTCTGAACCCAGCT15130.20939756334864487No Hit
GGTATAAGGGAGCTTGACTGCGAGA15020.20787517524763027No Hit
GTCCGTACCAGTTCTGAGTTGACTG14420.199571240151187No Hit
CTCTAACCCAGATAGGTGATCCCTA13870.19195929964611397No Hit
GCCCAACCCTTGGGACCGACTACAG13230.18310176887657445No Hit
GTACATGGGAAATCTCATCTTGAGG12220.16912347813089493No Hit
GATAGGGACCGAACTGTCTCACGAC12030.16649389868368789No Hit
TATCAACGCAGAGTACTTTTTTTTT11430.1581899635872446No Hit
TCGGTGGACTGCTCGAGCTGCTTCC10690.14794844363496457No Hit
CTACATAGCTACCCAGCGATGCCTT10400.14393487500501695No Hit
GTCTCAACGAGAGACTCGGTGAAAT10190.1410284977212618No Hit
CCCTTGGGACCGACTACAGCCCCAG10140.14033650312989154No Hit
GGGTTGGGCTGTTCGCCCATTAAAG10130.14019810421161746No Hit
GTCCCAAGGGTTGGGCTGTTCGCCC10050.13909091286542505No Hit
GGTATCAACGCAGAGTACATGGGCA9860.136461333418218No Hit
ATCGGAGACAGTGTCTGACGGGCAG9830.13604613666339582No Hit
GACCATCGCAATGCTTTGTTTTAAT9820.13590773774512177No Hit
GTCGTACTCATAACCGCATCAGGTC9810.13576933882684775No Hit
GTATCAACGCAGAGTACATGGGAAA9400.13009498317761148No Hit
GAGCAGATCCTCTCAAATTTCCTAC9360.12954138750451527No Hit
GTGCTGGAAGGTTAAGAGGAGTGCT9100.12594301562938984No Hit
GCCTCTGGTCGATGGAACAATGTAG8990.12442062752837525No Hit
CCCCCAAGAGTTCACATCGACGGGG8960.12400543077355308No Hit
GGATAGGTAGGAGTCTATGAGATCG8910.1233134361821828No Hit
GGCCTATTCACTGCGGCTGACTTAA8890.12303663834563469No Hit
GATTGGGAACGGGCCCTTCGGGGCC8820.12206784591771631No Hit
GTCCACTCTGGTCCTCTCGTACTAG8740.12096065457152386No Hit
GTATAGGGGGTGACGCCTGCCCGGT8660.11985346322533144No Hit
GTTCTGAACCCAGCTCGCGTGCCGC8470.11722388377812439No Hit
GGCTTATCTCCCCCAAGAGTTCACA8180.11321031514817681No Hit
AAATCACATTGCGTCAACATCCGCA8020.11099593245579191No Hit
GTTAAGAGGAGTGCTTAGCGCAAGC7970.11030393786442165No Hit
CTGGTACACCAGCGGTAAGTCCACT7920.10961194327305138No Hit
GAACAATGTAGGCAAGGGAAGTCGG7900.10933514543650326No Hit
TACCAGTTCTGAGTTGACTGTTCGA7880.10905834759995517No Hit
GCTTTGTTTTAATTAAACAGTCGGA7580.10490638005173351No Hit
CTCTTGGGGGAGATAAGCCTGTTAT7490.10366078978726702No Hit
CGTTGAGACAGTGCCCAAATCATTA7380.10213840168625242No Hit
ACTCATAACCGCATCAGGTCTCCAA7360.10186160384970432No Hit
TCCTGGGGCTGTAGTCGGTCCCAAG7280.10075441250351187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATC250.006029010319.0000024
CGCCGGC702.4900146E-816.28910312
GTGTAAA4850.015.66251
TGCACTG551.12441685E-515.5486885
CAGCCGA2500.015.20316210
GGTATCA6700.015.1642821
GACGGGT2450.015.12349815
AGGCGTC902.2773747E-914.77880315
GCGAAAT1700.014.53243512
CGGGTTC2550.014.52437717
TGTAAAG5200.014.427692
TTATCTA400.005275472514.254
CGAAATT1750.014.11722213
GTAAAGG5250.014.11232953
CGGTGAA1750.014.10939317
AGGTATA5150.014.0223347
ACAGCCG2600.013.8875059
CCGACGG2600.013.88750513
CTTATAC2950.013.8455373
GTGATCG551.9544079E-413.8210569
Produced by FastQC (version 0.11.5)