##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061982_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722549
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325320497294992	32.0	32.0	32.0	32.0	32.0
2	31.008476933744287	32.0	32.0	32.0	32.0	32.0
3	31.021878101000762	32.0	32.0	32.0	32.0	32.0
4	30.999916960649035	32.0	32.0	32.0	32.0	32.0
5	30.97539682429842	32.0	32.0	32.0	32.0	32.0
6	34.622523870353426	36.0	36.0	36.0	32.0	36.0
7	34.56409461503649	36.0	36.0	36.0	32.0	36.0
8	34.53466823703306	36.0	36.0	36.0	32.0	36.0
9	34.61234047794682	36.0	36.0	36.0	32.0	36.0
10	34.4277592246339	36.0	36.0	36.0	32.0	36.0
11	34.60321445327583	36.0	36.0	36.0	32.0	36.0
12	34.50788943033621	36.0	36.0	36.0	32.0	36.0
13	34.54994470963215	36.0	36.0	36.0	32.0	36.0
14	34.49293404322752	36.0	36.0	36.0	32.0	36.0
15	34.44182470669809	36.0	36.0	36.0	32.0	36.0
16	34.446587013475906	36.0	36.0	36.0	32.0	36.0
17	34.41084964479918	36.0	36.0	36.0	32.0	36.0
18	34.409936211938565	36.0	36.0	36.0	32.0	36.0
19	34.3713796572966	36.0	36.0	36.0	32.0	36.0
20	34.37194432488316	36.0	36.0	36.0	32.0	36.0
21	34.3457274177945	36.0	36.0	36.0	32.0	36.0
22	34.327054635740964	36.0	36.0	36.0	32.0	36.0
23	34.33584988699728	36.0	36.0	36.0	32.0	36.0
24	34.29023637151252	36.0	36.0	36.0	32.0	36.0
25	33.9970811668136	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	11.0
5	56.0
6	137.0
7	31.0
8	133.0
9	103.0
10	41.0
11	13.0
12	26.0
13	29.0
14	107.0
15	117.0
16	240.0
17	308.0
18	494.0
19	687.0
20	896.0
21	1231.0
22	1829.0
23	2467.0
24	3489.0
25	4873.0
26	6635.0
27	8524.0
28	11622.0
29	15390.0
30	20613.0
31	28345.0
32	41359.0
33	60992.0
34	145452.0
35	366299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.158998873428125	17.22187929903425	12.460936743127773	24.15818508440985
2	16.87311257691552	18.575564739130794	38.02290788406552	26.52841479988817
3	20.05361581514308	22.12697287931044	27.11141681936702	30.707994486179462
4	13.833562635470503	14.791925611987566	33.963614318419005	37.41089743412292
5	15.756000044301205	35.503676999922476	30.70530285410506	18.03502010167126
6	32.86230238303624	35.78515006148699	16.32105624674562	15.031491308731152
7	31.244172465914556	30.960017401207278	17.3773775992707	20.418432533607465
8	30.40574055548936	30.788979995621702	17.54130977551535	21.263969673373587
9	27.70496846212684	14.619383875223093	17.778021040029266	39.897626622620805
10	18.49120667433513	25.273223665063682	27.355350139279626	28.880219521321564
11	38.419779778115014	21.44883548496629	19.827037814689312	20.30434692222938
12	25.749556883173756	23.971962033999773	27.17542991686449	23.103051165961975
13	29.30644553335846	19.803884614531828	24.54851333673311	26.3411565153766
14	23.654930650815427	20.02674204320295	23.12161394465921	33.196713361322416
15	28.145852764154068	24.649067251312058	20.96657737150713	26.238502613026743
16	27.200119719324693	26.737731540499492	20.83421090554367	25.22793783463214
17	23.52711314592309	26.000573676560045	22.92433705022164	27.547976127295225
18	24.991825446967933	24.864357840366218	24.35573437967612	25.788082332989724
19	28.32761195477202	23.023769349480645	23.74829259102256	24.90032610472478
20	26.843303130501994	22.692295438268893	24.1868299771297	26.27757145409941
21	29.236212215451406	20.33359699344359	23.795070987367865	26.63511980373714
22	27.211882417159185	23.811661305115226	23.28312593688609	25.693330340839506
23	26.703473570777568	24.09476346531554	23.964978960228045	25.236784003678842
24	25.856007358380268	25.92817821908358	22.112957786280056	26.102856636256096
25	25.873826552026497	24.23276553480969	22.536988631562615	27.356419281601198
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	51.0
2	136.0
3	221.0
4	221.0
5	221.0
6	440.0
7	659.0
8	659.0
9	659.0
10	714.0
11	769.0
12	769.0
13	769.0
14	702.5
15	636.0
16	636.0
17	636.0
18	1159.0
19	1682.0
20	1682.0
21	1682.0
22	2843.5
23	4005.0
24	4005.0
25	4005.0
26	7284.5
27	10564.0
28	10564.0
29	10564.0
30	13554.0
31	16544.0
32	16544.0
33	16544.0
34	22719.0
35	28894.0
36	28894.0
37	28894.0
38	36204.0
39	43514.0
40	43514.0
41	43514.0
42	61607.0
43	79700.0
44	79700.0
45	79700.0
46	90223.0
47	100746.0
48	100746.0
49	100746.0
50	110743.0
51	120740.0
52	120740.0
53	120740.0
54	111826.5
55	102913.0
56	102913.0
57	102913.0
58	88288.0
59	73663.0
60	73663.0
61	73663.0
62	63741.0
63	53819.0
64	53819.0
65	53819.0
66	44589.0
67	35359.0
68	35359.0
69	35359.0
70	30256.0
71	25153.0
72	25153.0
73	25153.0
74	19563.0
75	13973.0
76	13973.0
77	13973.0
78	10117.5
79	6262.0
80	6262.0
81	6262.0
82	3904.5
83	1547.0
84	1547.0
85	1547.0
86	1154.0
87	761.0
88	761.0
89	761.0
90	422.5
91	84.0
92	84.0
93	84.0
94	68.0
95	52.0
96	52.0
97	52.0
98	145.0
99	238.0
100	238.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.151967548221643E-4
2	4.151967548221643E-4
3	1.3839891827405478E-4
4	0.00927272752436167
5	0.030586160938566108
6	0.06158751863195438
7	0.10518317788828163
8	0.1116879270471622
9	0.12137585132634603
10	0.13549254099029961
11	0.13895251394715097
12	0.13230936586999636
13	0.12912619074969311
14	0.11611669243193196
15	0.13452374856238125
16	0.11916146863396115
17	0.12289823942736064
18	0.11030393786442165
19	0.09618724820046806
20	0.09023609471468372
21	0.09134328606087615
22	0.1028303962776227
23	0.08068656935377393
24	0.09009769579640965
25	0.08829850985884694
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	722549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.82956233546433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.81173530312095	10.885696736114799
2	12.494924182731767	4.7054790755129705
3	6.975635021037507	3.9404446337422137
4	4.471144281273785	3.3675875982039845
5	3.206427714770615	3.018781526471687
6	2.308978729037602	2.608623534580484
7	1.7767197450419432	2.34183986343423
8	1.4100426926023895	2.124038942081813
9	1.1693374517602373	1.9816301195201165
>10	7.259092482224661	25.38003868100529
>50	0.5579811981992931	7.343906355227647
>100	0.4792160422529549	17.760768260261777
>500	0.054473098504944174	6.9973845295841555
>1k	0.024292057441394024	7.543780144258813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAAGGTATAAGGGAGCTTGAC	3246	0.4492428887175818	No Hit
ATGTAGGCAAGGGAAGTCGGCAAAA	2537	0.35111805566127696	No Hit
GTATCAACGCAGAGTACATGGGCAG	2467	0.3414301313820931	No Hit
GTCGGTGGACTGCTCGAGCTGCTTC	2335	0.3231614741699179	No Hit
TTCCCAATCCGTCCCCCGACCGGCA	2297	0.31790231527550383	No Hit
GTTCTGAGTTGACTGTTCGACGCCC	2072	0.2867625586638415	No Hit
GTATAAGGGAGCTTGACTGCGAGAG	1974	0.2731994646729841	No Hit
CCCTTATACCTTTACACTCTGCGAA	1969	0.2725074700816138	No Hit
CCCCTTGTCCGTACCAGTTCTGAGT	1960	0.27126187981714733	No Hit
CCTTTACACTCTGCGAATGATTTCC	1859	0.2572835890714678	No Hit
TCCCTACATAGCTACCCAGCGATGC	1786	0.24718046803746183	No Hit
TATCAACGCAGAGTACATGGGCAGA	1746	0.24164451130649964	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1690	0.23389417188315256	No Hit
GTACATGGGCAGAAATCACATTGCG	1674	0.23167978919076768	No Hit
GTCCACCGACAGCCGACGGGTTCGG	1660	0.22974220433493092	No Hit
ACGCAGAGTACATGGGCAGAAATCA	1606	0.22226866274813198	No Hit
GTCCCGAACCCGTCGGCTGTCGGTG	1582	0.21894708870955465	No Hit
TCGCAATGCTTTGTTTTAATTAAAC	1538	0.21285753630549625	No Hit
GTCCTAAGGTAGCGAAATTCCTTGT	1532	0.21202714279585194	No Hit
GTCTCACGACGTTCTGAACCCAGCT	1513	0.20939756334864487	No Hit
GGTATAAGGGAGCTTGACTGCGAGA	1502	0.20787517524763027	No Hit
GTCCGTACCAGTTCTGAGTTGACTG	1442	0.199571240151187	No Hit
CTCTAACCCAGATAGGTGATCCCTA	1387	0.19195929964611397	No Hit
GCCCAACCCTTGGGACCGACTACAG	1323	0.18310176887657445	No Hit
GTACATGGGAAATCTCATCTTGAGG	1222	0.16912347813089493	No Hit
GATAGGGACCGAACTGTCTCACGAC	1203	0.16649389868368789	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1143	0.1581899635872446	No Hit
TCGGTGGACTGCTCGAGCTGCTTCC	1069	0.14794844363496457	No Hit
CTACATAGCTACCCAGCGATGCCTT	1040	0.14393487500501695	No Hit
GTCTCAACGAGAGACTCGGTGAAAT	1019	0.1410284977212618	No Hit
CCCTTGGGACCGACTACAGCCCCAG	1014	0.14033650312989154	No Hit
GGGTTGGGCTGTTCGCCCATTAAAG	1013	0.14019810421161746	No Hit
GTCCCAAGGGTTGGGCTGTTCGCCC	1005	0.13909091286542505	No Hit
GGTATCAACGCAGAGTACATGGGCA	986	0.136461333418218	No Hit
ATCGGAGACAGTGTCTGACGGGCAG	983	0.13604613666339582	No Hit
GACCATCGCAATGCTTTGTTTTAAT	982	0.13590773774512177	No Hit
GTCGTACTCATAACCGCATCAGGTC	981	0.13576933882684775	No Hit
GTATCAACGCAGAGTACATGGGAAA	940	0.13009498317761148	No Hit
GAGCAGATCCTCTCAAATTTCCTAC	936	0.12954138750451527	No Hit
GTGCTGGAAGGTTAAGAGGAGTGCT	910	0.12594301562938984	No Hit
GCCTCTGGTCGATGGAACAATGTAG	899	0.12442062752837525	No Hit
CCCCCAAGAGTTCACATCGACGGGG	896	0.12400543077355308	No Hit
GGATAGGTAGGAGTCTATGAGATCG	891	0.1233134361821828	No Hit
GGCCTATTCACTGCGGCTGACTTAA	889	0.12303663834563469	No Hit
GATTGGGAACGGGCCCTTCGGGGCC	882	0.12206784591771631	No Hit
GTCCACTCTGGTCCTCTCGTACTAG	874	0.12096065457152386	No Hit
GTATAGGGGGTGACGCCTGCCCGGT	866	0.11985346322533144	No Hit
GTTCTGAACCCAGCTCGCGTGCCGC	847	0.11722388377812439	No Hit
GGCTTATCTCCCCCAAGAGTTCACA	818	0.11321031514817681	No Hit
AAATCACATTGCGTCAACATCCGCA	802	0.11099593245579191	No Hit
GTTAAGAGGAGTGCTTAGCGCAAGC	797	0.11030393786442165	No Hit
CTGGTACACCAGCGGTAAGTCCACT	792	0.10961194327305138	No Hit
GAACAATGTAGGCAAGGGAAGTCGG	790	0.10933514543650326	No Hit
TACCAGTTCTGAGTTGACTGTTCGA	788	0.10905834759995517	No Hit
GCTTTGTTTTAATTAAACAGTCGGA	758	0.10490638005173351	No Hit
CTCTTGGGGGAGATAAGCCTGTTAT	749	0.10366078978726702	No Hit
CGTTGAGACAGTGCCCAAATCATTA	738	0.10213840168625242	No Hit
ACTCATAACCGCATCAGGTCTCCAA	736	0.10186160384970432	No Hit
TCCTGGGGCTGTAGTCGGTCCCAAG	728	0.10075441250351187	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3839891827405478E-4	0.0
5	0.0	0.0	0.0	1.3839891827405478E-4	0.0
6	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
7	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
8	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
9	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
10	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
11	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
12	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
13	0.0	0.0	1.3839891827405478E-4	1.3839891827405478E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGATC	25	0.0060290103	19.000002	4
CGCCGGC	70	2.4900146E-8	16.289103	12
GTGTAAA	485	0.0	15.6625	1
TGCACTG	55	1.12441685E-5	15.548688	5
CAGCCGA	250	0.0	15.203162	10
GGTATCA	670	0.0	15.164282	1
GACGGGT	245	0.0	15.123498	15
AGGCGTC	90	2.2773747E-9	14.778803	15
GCGAAAT	170	0.0	14.532435	12
CGGGTTC	255	0.0	14.524377	17
TGTAAAG	520	0.0	14.42769	2
TTATCTA	40	0.0052754725	14.25	4
CGAAATT	175	0.0	14.117222	13
GTAAAGG	525	0.0	14.1123295	3
CGGTGAA	175	0.0	14.109393	17
AGGTATA	515	0.0	14.022334	7
ACAGCCG	260	0.0	13.887505	9
CCGACGG	260	0.0	13.887505	13
CTTATAC	295	0.0	13.845537	3
GTGATCG	55	1.9544079E-4	13.821056	9
>>END_MODULE