FastQCFastQC Report
Thu 2 Feb 2017
SRR4061982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722549
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG31680.4384477730922055No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA24080.33326459520392393No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT18890.2614355566196895No Hit
GTCGGTGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGTCGCGTG18560.25686839231664566No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC18290.25313162152324614No Hit
TCCCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCA17630.24399729291715855No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC17570.2431668994075142No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCA16380.22669742813290172No Hit
GGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAG15110.20912076551209677No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGAG14800.20483039904560107No Hit
GTTCTGAGTTGACTGTTCGACGCCCGGGGAAGAGGCCCCGAAGGGCCCGT14370.1988792455598167No Hit
GCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGACGAGCCGACATC14290.19777205421362426No Hit
TTCCCAATCCGTCCCCCGACCGGCACGCGACGACCCGCTCTCGCCGCGGA13860.19182090072783992No Hit
GTCTCACGACGTTCTGAACCCAGCTCGCGTGCCGCTTTAATGGGCGAACA13850.19168250180956584No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA13390.18531615156895934No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT13060.1807489872659155No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTTCCGCGGCG13020.1801953915928193No Hit
CTCTAACCCAGATAGGTGATCCCTATCGGAGACAGTGTCTGACGGGCAGT12820.1774274132273382No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA12170.16843148353952467No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAG11730.16234193113546624No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTGCCGC11540.1597123516882592No Hit
GTACATGGGAAATCTCATCTTGAGGTGGGTTTCACACTTAGATGCTTTCA11450.1584667614237927No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACCATCGCAA10790.14933243281770509No Hit
CCCTTGGGACCGACTACAGCCCCAGGATGCGACGAGCCGACATCGAGGTG10750.14877883714460888No Hit
CTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCG10350.1432428804136467No Hit
GTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGAGGCCCCG10170.1407516998847137No Hit
GTCTCAACGAGAGACTCGGTGAAATTTTAGTACCTGTGAAGATGCAGGTT10080.1395061096202472No Hit
TCGGTGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGTCGCGTGC9870.13659973233649206No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCGAGCTGGGTTCAGAACG9830.13604613666339582No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACC9720.13452374856238125No Hit
GTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCGAGCTGGGTT9700.13424695072583312No Hit
ATCGGAGACAGTGTCTGACGGGCAGTTTGACTGGGGCGGTCGCCTCCTAA9600.13286296154309257No Hit
GAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGATAGGGACCGAACT9000.12455902644664929No Hit
GTATCAACGCAGAGTACATGGGAAATCTCATCTTGAGGTGGGTTTCACAC8800.1217910480811682No Hit
CCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTC8570.11860787296086495No Hit
GTCCACTCTGGTCCTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTAC8460.11708548485985033No Hit
GGCCTATTCACTGCGGCTGACTTAAAGTCAGCACCCCTTCTCCCGAAGTT8430.11667028810502816No Hit
GTTCTGAACCCAGCTCGCGTGCCGCTTTAATGGGCGAACAGCCCAACCCT8270.1144559054126433No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC8030.11113433137406599No Hit
CGTTGAGACAGTGCCCAAATCATTACGCCTTTCGTGCGGGTCGGAACTTA8010.11085753353751787No Hit
GATTGGGAACGGGCCCTTCGGGGCCTCTTCCCCGGGCGTCGAACAGTCAA7810.10808955517203676No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA7670.10615197031620001No Hit
CTGGTACACCAGCGGTAAGTCCACTCTGGTCCTCTCGTACTAGGAGCAGA7630.1055983746431038No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG7580.10490638005173351No Hit
GTTAAGAGGAGTGCTTAGCGCAAGCGAAGGTATGAATTGAAGCCCCAGTA7540.1043527843786373No Hit
ATCCTGTACATGTGGTACAGACACTCAATATCAAACTGCAGTAAAGCTCC7380.10213840168625242No Hit
GTATAGGGGGTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGCTTA7230.1000624179121416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAG250.002344591335.2231561
AAATATG451.0367294E-634.2210424
CCAGAAA300.00573670729.3404444
TATAGAT300.005740589529.3363782
CTAAGAT300.005744474529.3323216
AACTGGA300.005744474529.3323235
TACAGTG300.005744474529.332327
GTATCAA19000.028.8505461
AGGTATA6100.028.4908167
TAAATAT554.958758E-627.99903323
AACCTAA407.030797E-427.4990487
TATACCT3200.027.4990485
GGTATCA6900.027.4383321
GTGTAAA6500.027.0947361
TTATACC3250.027.0759854
CAGTGCA508.3388346E-526.3990869
TAAAGGT6750.026.0731724
CCTTATA3500.025.1454682
GTAAAGG7050.024.6516283
TGTAAAG7100.024.4814172
Produced by FastQC (version 0.11.5)