FastQCFastQC Report
Thu 2 Feb 2017
SRR4061981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25876
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAG950.3671355696398207No Hit
GTATCAACGCAGAGTACTTTTTTTT910.3516772298655125No Hit
GTGTAGCCCTGGCTGTCCTGGAACT810.3130313804297419No Hit
CTATAATACCTTGGAATACTGCCTG600.2318750966146236No Hit
CTGTAGACCAGGCTGGCCTCGAACT580.2241459267274695No Hit
GCTGAAGGGAGCATCAAAGAAATCA560.21641675684031533No Hit
GTATCAACGCAGAGTACATGGGGAG500.19322924717885298No Hit
TATCAACGCAGAGTACTTTTTTTTT500.19322924717885298No Hit
CTCCAGGAAAGAGTGGTTGCACATG490.18936466223527593No Hit
CGGTAGAACATGGCGCAGGGACAAC470.18163549234812182No Hit
CCTAAATAGAATCTAAGTGGCATTT430.16617715257381357No Hit
ACACTGGAGCATCCCCAAGTGGCTC420.1623125676302365No Hit
GTTGTAGAGAATAGTTTGAAAATGA420.1623125676302365No Hit
GTACATGGGTTGCGGTAGAACATGG410.15844798268665944No Hit
CCCTTCAGCAGTGCAAGCCTCTTAG400.15458339774308238No Hit
ATACTAGCCTAACCTTTTTGTTTTT390.15071881279950533No Hit
GTTTAGGAGATTGTAAAGGGAGGTT380.14685422785592828No Hit
GATTAAAAGCGTGCGCCACCATCGC370.1429896429123512No Hit
GTATTGGAATTAGTGAAATTGGAGT350.1352604730251971No Hit
CTCCAGAGGCATCGTGGGGATGCCA330.12753130313804295No Hit
GGTATCAACGCAGAGTACTTTTTTT320.12366671819446591No Hit
GTATCAACGCAGAGTACATGGGTTG320.12366671819446591No Hit
GCTATGGAGTGCCAATTGAGACTTC310.11980213325088886No Hit
GTAGAACATGGCGCAGGGACAACGC310.11980213325088886No Hit
ATTCTGCATTGCTCAAAACATAGAA300.1159375483073118No Hit
GTAGAGAATAGTTTGAAAATGAGGT300.1159375483073118No Hit
GGGTTTCTCTGTGTAGCCCTGGCTG300.1159375483073118No Hit
ATCCTGAAGGCACTGGGACTTTGCT300.1159375483073118No Hit
GTGCTATGGAGTGCCAATTGAGACT300.1159375483073118No Hit
TTCCTGGAGTCCTCTAGTGACAAAT290.11207296336373475No Hit
GCCTAACACTTCCATCTTCCCTGTT290.11207296336373475No Hit
GCTTTATTGGGTAACACTGGAGCAT280.10820837842015767No Hit
GTACATGGGACTGTGCCTGCAACCT280.10820837842015767No Hit
GTACATGGGAACGCCGCCTTGTCGA280.10820837842015767No Hit
GTACTTTTTTTTTTTTTTTTTTTTT280.10820837842015767No Hit
GCATAAGACATGCTGTGATACACCA280.10820837842015767No Hit
CCCCACAGGCTGGCTGGACTTGGAA270.10434379347658061No Hit
CATTTACACCTACTACCCAACTATC260.10047920853300356No Hit
TATTTAAACAGTTTGAAAAAGGAAA260.10047920853300356No Hit
ACGCAGAGTACTTTTTTTTTTTTTT260.10047920853300356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA551.8403183E-413.8224181
TATCAAC657.544491E-411.6958932
TCAACGC700.001403445610.8604714
ATCAACG700.001403445610.8604713
CAACGCA700.001403445610.8604715
AACGCAG750.00249331110.136446
Produced by FastQC (version 0.11.5)