##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061981_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25876
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166099860874944	32.0	32.0	32.0	32.0	32.0
2	30.875946823311175	32.0	32.0	32.0	32.0	32.0
3	30.800200958417065	32.0	32.0	32.0	32.0	32.0
4	30.812103880043285	32.0	32.0	32.0	32.0	32.0
5	30.85519400216417	32.0	32.0	32.0	32.0	32.0
6	34.207489565620655	36.0	36.0	36.0	32.0	36.0
7	34.23253207605503	36.0	36.0	36.0	32.0	36.0
8	34.122932447055184	36.0	36.0	36.0	32.0	36.0
9	34.32385221827176	36.0	36.0	36.0	32.0	36.0
10	34.1131164012985	36.0	36.0	36.0	32.0	36.0
11	34.28528366053486	36.0	36.0	36.0	32.0	36.0
12	34.15419693924873	36.0	36.0	36.0	32.0	36.0
13	34.243275622198176	36.0	36.0	36.0	32.0	36.0
14	34.14016849590354	36.0	36.0	36.0	32.0	36.0
15	34.031148554645235	36.0	36.0	36.0	32.0	36.0
16	34.06078992116247	36.0	36.0	36.0	32.0	36.0
17	34.07191992579997	36.0	36.0	36.0	32.0	36.0
18	34.00320760550317	36.0	36.0	36.0	32.0	36.0
19	34.07010357087649	36.0	36.0	36.0	32.0	36.0
20	34.02678157365899	36.0	36.0	36.0	32.0	36.0
21	33.935963827484926	36.0	36.0	36.0	32.0	36.0
22	33.94307466378111	36.0	36.0	36.0	32.0	36.0
23	33.932949451228936	36.0	36.0	36.0	32.0	36.0
24	33.90790694079456	36.0	36.0	36.0	32.0	36.0
25	33.304142835059515	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	4.0
7	1.0
8	5.0
9	2.0
10	2.0
11	0.0
12	2.0
13	1.0
14	8.0
15	24.0
16	21.0
17	22.0
18	28.0
19	33.0
20	50.0
21	54.0
22	69.0
23	96.0
24	137.0
25	194.0
26	245.0
27	335.0
28	422.0
29	627.0
30	878.0
31	1292.0
32	2025.0
33	3106.0
34	7000.0
35	9188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25428968928737	17.066007110836296	12.55217189673829	25.127531303138046
2	17.491111454629774	19.264955943731643	36.98794249497604	26.255990106662548
3	19.489101870459113	24.509197712165715	28.002782501159373	27.9989179162158
4	13.528138528138529	15.820191713048857	35.08039579468151	35.57127396413111
5	14.601615958557234	36.79978350794448	33.834615533304984	14.763985000193298
6	34.3775379974475	35.32505704451406	16.09622152608578	14.201183431952662
7	30.057646922273378	30.502572832437036	19.979107826827097	19.46067241846249
8	29.223090613634607	31.939178209394104	18.351002089298152	20.48672908767314
9	28.98270324652711	14.731261850404366	19.018689780598226	37.2673451224703
10	17.609040597546343	28.112543055071793	29.695421649444636	24.582994697937227
11	35.81517743121396	22.208892844704152	22.092798266320962	19.88313145776092
12	26.021513697570036	23.66893669710571	27.588608574524066	22.720941030800184
13	29.433159218417487	20.437221899787193	23.041207196749856	27.088411685045465
14	25.13927576601671	18.446301454658002	26.203187867533273	30.21123491179202
15	27.288909527126382	26.21314139772463	21.434099527900315	25.06384954724866
16	26.361807489941196	25.53775920767564	23.692355307954195	24.40807799442897
17	23.77930821016792	26.05819082256442	23.91085661224174	26.25164435502592
18	25.062869965566602	25.65094595117422	25.047394281734825	24.238789801524355
19	25.99412037753365	24.311465263809374	25.29011295064212	24.404301408014852
20	27.07229335086837	24.318260936835184	24.372413259583027	24.237032452713418
21	27.388781431334625	24.21276595744681	24.460348162475825	23.93810444874275
22	25.987542074515417	24.432235849421595	23.937013966804656	25.643208109258325
23	24.82980040228996	25.878075197276807	24.528082933622155	24.76404146681108
24	24.978726696062505	24.251566488744487	25.338438926278332	25.431267888914675
25	25.057053340037903	24.569682435307314	26.29868874018489	24.074575484469886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	4.5
3	8.0
4	8.0
5	8.0
6	23.5
7	39.0
8	39.0
9	39.0
10	35.5
11	32.0
12	32.0
13	32.0
14	44.5
15	57.0
16	57.0
17	57.0
18	159.0
19	261.0
20	261.0
21	261.0
22	367.0
23	473.0
24	473.0
25	473.0
26	725.5
27	978.0
28	978.0
29	978.0
30	1149.0
31	1320.0
32	1320.0
33	1320.0
34	1359.0
35	1398.0
36	1398.0
37	1398.0
38	1565.5
39	1733.0
40	1733.0
41	1733.0
42	2003.0
43	2273.0
44	2273.0
45	2273.0
46	2607.0
47	2941.0
48	2941.0
49	2941.0
50	3105.0
51	3269.0
52	3269.0
53	3269.0
54	3275.0
55	3281.0
56	3281.0
57	3281.0
58	3189.5
59	3098.0
60	3098.0
61	3098.0
62	2576.0
63	2054.0
64	2054.0
65	2054.0
66	1632.5
67	1211.0
68	1211.0
69	1211.0
70	926.0
71	641.0
72	641.0
73	641.0
74	551.0
75	461.0
76	461.0
77	461.0
78	329.5
79	198.0
80	198.0
81	198.0
82	136.5
83	75.0
84	75.0
85	75.0
86	55.0
87	35.0
88	35.0
89	35.0
90	30.0
91	25.0
92	25.0
93	25.0
94	13.0
95	1.0
96	1.0
97	1.0
98	7.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.015458339774308239
5	0.034781264492193535
6	0.07342711392796414
7	0.11207296336373475
8	0.1159375483073118
9	0.12753130313804295
10	0.1429896429123512
11	0.1352604730251971
12	0.12366671819446591
13	0.11980213325088886
14	0.10820837842015767
15	0.13139588808162003
16	0.10820837842015767
17	0.1159375483073118
18	0.11207296336373475
19	0.09275003864584944
20	0.08888545370227237
21	0.10047920853300356
22	0.11207296336373475
23	0.09275003864584944
24	0.08502086875869531
25	0.08888545370227237
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	25876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.644767351986395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.34001484780994	25.544906477044364
2	15.358203414996288	12.791776163240067
3	8.092056421677803	10.109754212397588
4	3.78619153674833	6.307002627917761
5	2.830363771343727	5.893492038955016
6	1.8466963622865626	4.6143144226310095
7	1.3734224201930216	4.0037100015458345
8	1.1321455085374907	3.77183490493121
9	0.7887899034892353	2.9564074818364507
>10	3.3964365256124722	22.302519709383212
>50	0.05567928730512249	1.7042819601174832
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAATGGAGGCTGACCAGAGCAG	95	0.3671355696398207	No Hit
GTATCAACGCAGAGTACTTTTTTTT	91	0.3516772298655125	No Hit
GTGTAGCCCTGGCTGTCCTGGAACT	81	0.3130313804297419	No Hit
CTATAATACCTTGGAATACTGCCTG	60	0.2318750966146236	No Hit
CTGTAGACCAGGCTGGCCTCGAACT	58	0.2241459267274695	No Hit
GCTGAAGGGAGCATCAAAGAAATCA	56	0.21641675684031533	No Hit
GTATCAACGCAGAGTACATGGGGAG	50	0.19322924717885298	No Hit
TATCAACGCAGAGTACTTTTTTTTT	50	0.19322924717885298	No Hit
CTCCAGGAAAGAGTGGTTGCACATG	49	0.18936466223527593	No Hit
CGGTAGAACATGGCGCAGGGACAAC	47	0.18163549234812182	No Hit
CCTAAATAGAATCTAAGTGGCATTT	43	0.16617715257381357	No Hit
ACACTGGAGCATCCCCAAGTGGCTC	42	0.1623125676302365	No Hit
GTTGTAGAGAATAGTTTGAAAATGA	42	0.1623125676302365	No Hit
GTACATGGGTTGCGGTAGAACATGG	41	0.15844798268665944	No Hit
CCCTTCAGCAGTGCAAGCCTCTTAG	40	0.15458339774308238	No Hit
ATACTAGCCTAACCTTTTTGTTTTT	39	0.15071881279950533	No Hit
GTTTAGGAGATTGTAAAGGGAGGTT	38	0.14685422785592828	No Hit
GATTAAAAGCGTGCGCCACCATCGC	37	0.1429896429123512	No Hit
GTATTGGAATTAGTGAAATTGGAGT	35	0.1352604730251971	No Hit
CTCCAGAGGCATCGTGGGGATGCCA	33	0.12753130313804295	No Hit
GGTATCAACGCAGAGTACTTTTTTT	32	0.12366671819446591	No Hit
GTATCAACGCAGAGTACATGGGTTG	32	0.12366671819446591	No Hit
GCTATGGAGTGCCAATTGAGACTTC	31	0.11980213325088886	No Hit
GTAGAACATGGCGCAGGGACAACGC	31	0.11980213325088886	No Hit
ATTCTGCATTGCTCAAAACATAGAA	30	0.1159375483073118	No Hit
GTAGAGAATAGTTTGAAAATGAGGT	30	0.1159375483073118	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTG	30	0.1159375483073118	No Hit
ATCCTGAAGGCACTGGGACTTTGCT	30	0.1159375483073118	No Hit
GTGCTATGGAGTGCCAATTGAGACT	30	0.1159375483073118	No Hit
TTCCTGGAGTCCTCTAGTGACAAAT	29	0.11207296336373475	No Hit
GCCTAACACTTCCATCTTCCCTGTT	29	0.11207296336373475	No Hit
GCTTTATTGGGTAACACTGGAGCAT	28	0.10820837842015767	No Hit
GTACATGGGACTGTGCCTGCAACCT	28	0.10820837842015767	No Hit
GTACATGGGAACGCCGCCTTGTCGA	28	0.10820837842015767	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	28	0.10820837842015767	No Hit
GCATAAGACATGCTGTGATACACCA	28	0.10820837842015767	No Hit
CCCCACAGGCTGGCTGGACTTGGAA	27	0.10434379347658061	No Hit
CATTTACACCTACTACCCAACTATC	26	0.10047920853300356	No Hit
TATTTAAACAGTTTGAAAAAGGAAA	26	0.10047920853300356	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	26	0.10047920853300356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	55	1.8403183E-4	13.822418	1
TATCAAC	65	7.544491E-4	11.695893	2
TCAACGC	70	0.0014034456	10.860471	4
ATCAACG	70	0.0014034456	10.860471	3
CAACGCA	70	0.0014034456	10.860471	5
AACGCAG	75	0.002493311	10.13644	6
>>END_MODULE