FastQCFastQC Report
Thu 2 Feb 2017
SRR4061981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25876
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT730.2821147008811254No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC690.2666563611068171No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG580.2241459267274695No Hit
CTATAATACCTTGGAATACTGCCTGTTGCATGTGCTGGCCACATGCCTCC540.20868758695316122No Hit
CGGTAGAACATGGCGCAGGGACAACGCAAGTTCCAGGCGCAGAAACCTAA500.19322924717885298No Hit
CCTAAATAGAATCTAAGTGGCATTTAAAACAGTAAAGTTGTAGAGAATAG490.18936466223527593No Hit
GCTGAAGGGAGCATCAAAGAAATCAGAAGCCACCATCCCTGGCAAGACAC470.18163549234812182No Hit
CTCCAGGAAAGAGTGGTTGCACATGAAGATAAAAGGAAAAGGGTGGGGGT430.16617715257381357No Hit
ACACTGGAGCATCCCCAAGTGGCTCTGGATTACTGGTGTGACAGGCTGAC420.1623125676302365No Hit
CTCCAGAGGCATCGTGGGGATGCCAGATTGGGGAATCCTCCATGACCTGG420.1623125676302365No Hit
GTACATGGGTTGCGGTAGAACATGGCGCAGGGACAACGCAAGTTCCAGGC410.15844798268665944No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT380.14685422785592828No Hit
GCCCTACTGTGTGCAGAAAGAGCTCAGGGACTGCAGTGCTACGAGTGCTA370.1429896429123512No Hit
ATACTAGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGC370.1429896429123512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.13912505796877414No Hit
TCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGG360.13912505796877414No Hit
GATTAAAAGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT350.1352604730251971No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA320.12366671819446591No Hit
GTTTTAAAAGATTGAGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAAT320.12366671819446591No Hit
GTAGAACATGGCGCAGGGACAACGCAAGTTCCAGGCGCAGAAACCTAAAA320.12366671819446591No Hit
GTACATGGGACTGTGCCTGCAACCTTGTCTGAGAGGAACCCTTCTCTGAG310.11980213325088886No Hit
GCTCAAAACATAGAATTGATTGAGGACTCTCAAAGAAGGAAACTAAAGAC300.1159375483073118No Hit
GCCTAAGAGGCTTGCACTGCTGAAGGGAGCATCAAAGAAATCAGAAGCCA300.1159375483073118No Hit
CTATTAGCTTAAGTTTAGAGTTCTAATTCTTTTTACTGCTCAATCTTTTT290.11207296336373475No Hit
GTCCAGGTGCTACCTGCAGTGGGAACTGCTGCATTGCAGAGGTCTTCGCT290.11207296336373475No Hit
CCCCACAGGCTGGCTGGACTTGGAAGAGGGCCCCTGCTCCAGTCCTGGTG280.10820837842015767No Hit
CCCTTCAGCAGTGCAAGCCTCTTAGGCAGGCTGGAGCTGGCCTTCATTAC280.10820837842015767No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT280.10820837842015767No Hit
GTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGA260.10047920853300356No Hit
GTTTAATGGAGACAGGCTGGGTAAGTTGACGACGGTTCAGGTGGCTTTGA260.10047920853300356No Hit
GTACATGGGAGTGTGGGGCCTCACCGCGACGCCGCGGTCATGAGCCGGCC260.10047920853300356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA459.860887E-734.222221
ATGGGGA353.1322378E-431.4285725
CTATAAT300.005642949629.3333321
GTACTTT300.005642949629.33333214
TTATTTA300.005642949629.33333225
AGCAGCA300.005642949629.33333244
GCTGCAT300.005642949629.33333239
TGGGGAG406.8464805E-427.56
CATGGGG609.28048E-625.6666664
AGTACTT450.00136159124.44444513
GTACATG1407.2759576E-1222.01
TATCAAC755.212091E-520.5333332
TACATGG1552.7284841E-1119.8709682
AGTACAT707.8335626E-418.85714312
GAGTACA707.8335626E-418.85714311
ACATGGG1656.91216E-1118.6666663
TCAACGC851.3635066E-418.1176474
ATCAACG902.1117424E-417.111113
ACGCAGA953.190703E-416.2105267
CAGAGTA953.190703E-416.21052610
Produced by FastQC (version 0.11.5)