##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25876
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206175606739837	32.0	32.0	32.0	32.0	32.0
2	31.253864584943578	32.0	32.0	32.0	32.0	32.0
3	31.451035708764877	32.0	32.0	32.0	32.0	32.0
4	31.534394805997835	32.0	32.0	32.0	32.0	32.0
5	31.458764878652033	32.0	32.0	32.0	32.0	32.0
6	35.09823774926573	36.0	36.0	36.0	36.0	36.0
7	35.10113618797341	36.0	36.0	36.0	36.0	36.0
8	35.089503787293246	36.0	36.0	36.0	36.0	36.0
9	35.14619724841552	36.0	36.0	36.0	36.0	36.0
10	35.003400834750344	36.0	36.0	36.0	36.0	36.0
11	35.131975575823155	36.0	36.0	36.0	36.0	36.0
12	35.04788220745092	36.0	36.0	36.0	36.0	36.0
13	35.130622971092905	36.0	36.0	36.0	36.0	36.0
14	35.08745555727315	36.0	36.0	36.0	36.0	36.0
15	35.07845107435462	36.0	36.0	36.0	36.0	36.0
16	35.05688669036945	36.0	36.0	36.0	36.0	36.0
17	35.031380429741844	36.0	36.0	36.0	36.0	36.0
18	34.97607821919926	36.0	36.0	36.0	36.0	36.0
19	35.03941876642448	36.0	36.0	36.0	36.0	36.0
20	35.01797031998763	36.0	36.0	36.0	36.0	36.0
21	34.982686659452774	36.0	36.0	36.0	36.0	36.0
22	34.990377183490494	36.0	36.0	36.0	36.0	36.0
23	34.90740454475189	36.0	36.0	36.0	32.0	36.0
24	34.90493121038801	36.0	36.0	36.0	32.0	36.0
25	34.931403617251505	36.0	36.0	36.0	32.0	36.0
26	34.868024424176845	36.0	36.0	36.0	32.0	36.0
27	34.84738754057814	36.0	36.0	36.0	32.0	36.0
28	34.7896506415211	36.0	36.0	36.0	32.0	36.0
29	34.76874323697635	36.0	36.0	36.0	32.0	36.0
30	34.76136187973412	36.0	36.0	36.0	32.0	36.0
31	34.811640129850055	36.0	36.0	36.0	32.0	36.0
32	34.75912042046684	36.0	36.0	36.0	32.0	36.0
33	34.701267583861494	36.0	36.0	36.0	32.0	36.0
34	34.72433915597465	36.0	36.0	36.0	32.0	36.0
35	34.66269902612459	36.0	36.0	36.0	32.0	36.0
36	34.58049930437471	36.0	36.0	36.0	32.0	36.0
37	34.65489256453857	36.0	36.0	36.0	32.0	36.0
38	34.59966764569485	36.0	36.0	36.0	32.0	36.0
39	34.64812954088731	36.0	36.0	36.0	32.0	36.0
40	34.56635492348122	36.0	36.0	36.0	32.0	36.0
41	34.525738135724225	36.0	36.0	36.0	32.0	36.0
42	34.53528366053486	36.0	36.0	36.0	32.0	36.0
43	34.47109290462205	36.0	36.0	36.0	32.0	36.0
44	34.470281341783895	36.0	36.0	36.0	32.0	36.0
45	34.3927191219663	36.0	36.0	36.0	32.0	36.0
46	34.33436388931829	36.0	36.0	36.0	32.0	36.0
47	34.33579378574741	36.0	36.0	36.0	32.0	36.0
48	34.35260473025197	36.0	36.0	36.0	32.0	36.0
49	34.29092595455248	36.0	36.0	36.0	32.0	36.0
50	33.83745555727315	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	4.0
23	6.0
24	17.0
25	40.0
26	79.0
27	151.0
28	224.0
29	378.0
30	591.0
31	869.0
32	1376.0
33	2270.0
34	5175.0
35	14694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1608040201005	18.33011209895632	12.357943563973715	27.151140316969464
2	16.606121502550625	20.559591899829957	36.48554645231103	26.34874014530839
3	18.92487246869686	25.0	28.6558973566239	27.41923017467924
4	13.750193229247179	16.146235894264958	34.71170196320915	35.391868913278714
5	14.5888081620034	38.05070335445973	32.98036790848663	14.38012057505024
6	34.98222290925955	35.13294172205905	16.10372545988561	13.781109908795797
7	30.275158447982687	30.78528366053486	19.431133096305455	19.508424795177
8	30.263564693151956	32.13015922089967	18.051476271448447	19.554799814499923
9	29.397897665790694	14.476735198639664	18.4804452001855	37.64492193538414
10	18.063070026279178	27.882980367908488	29.695470706446127	24.358478899366208
11	35.70876487865203	22.101561292317204	22.491884371618486	19.697789457412274
12	25.72267738444891	23.573968155820065	27.68202195084248	23.021332508888545
13	29.70706446127686	19.80986242077601	23.77492657288607	26.708146545061062
14	25.22028134178389	18.87849744937394	25.517854382439324	30.383366826402845
15	26.866594527747722	27.079146699644458	21.077446282269285	24.97681249033854
16	26.619261091358787	25.332354305147625	23.523728551553564	24.52465605194002
17	24.292780955325398	25.5217189673829	24.053176688823623	26.132323388468077
18	25.189364662235274	25.042510434379352	25.44442726851136	24.323697634874016
19	27.117792549080228	24.32756221981759	24.9497603957335	23.60488483536868
20	27.26078219199258	24.269593445663936	23.944968310403464	24.52465605194002
21	27.438553099397122	24.466687277786367	24.27345803060751	23.821301592208997
22	26.92842788684495	24.176843407018087	23.863812026588345	25.030916679548614
23	25.347812644921934	25.97387540578142	24.223218426341013	24.455093522955636
24	25.704347826086956	24.35169082125604	24.92753623188406	25.016425120772944
25	25.417375173906322	24.72174988406245	25.74586489411037	24.115010047920855
26	25.45412383087269	23.66468269305094	26.126613588930976	24.754579887145397
27	26.24439635183181	24.354614314422633	23.523728551553564	25.87726078219199
28	26.17299219293499	24.5188219834583	24.561335703795315	24.746850119811395
29	26.466723351627113	24.368091520445233	24.039576408750097	25.12560871917755
30	26.118883821596974	24.19030687176316	24.259874777769188	25.43093452887068
31	26.279177616324006	24.23481218117174	24.443499768124905	25.042510434379352
32	25.782638942567825	24.561335703795315	25.156527788513568	24.499497565123292
33	27.297673340032464	23.139058514338718	23.37481641802582	26.188451727603002
34	25.3487922705314	24.718840579710143	24.173913043478258	25.758454106280194
35	26.731334054722524	24.84541660225692	24.246405936002475	24.176843407018087
36	26.016385840160765	23.979749574895656	25.896583706909876	24.1072808780337
37	26.357487922705314	23.157487922705315	25.093719806763286	25.39130434782609
38	26.04730251970938	24.03771834904931	24.543978976657908	25.371000154583395
39	27.036636265265113	24.323697634874016	23.693770289070955	24.94589581078992
40	26.499458958107898	24.13046838769516	24.49760395733498	24.872468696861958
41	26.697584541062803	23.416425120772946	24.5024154589372	25.383574879227055
42	27.236887875391336	23.71970780350172	23.79700846442237	25.246395856684572
43	25.5604514533086	24.331323438466296	24.741032776747062	25.367192331478044
44	27.078421520503998	24.140996405519267	24.809647122482897	23.970934951493835
45	26.508212560386475	25.055072463768113	23.6792270531401	24.757487922705316
46	24.730434782608697	24.19710144927536	24.266666666666666	26.805797101449276
47	25.641521100633792	24.748801978667494	24.47441644767352	25.135260473025195
48	25.67541452479419	24.137131372473235	24.017315348046225	26.170138754686352
49	26.06191782939744	24.597070304951107	24.620260503227303	24.72075136242415
50	26.37579223991343	23.809707837378266	24.246405936002475	25.568093986705826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.5
6	3.0
7	4.0
8	5.0
9	6.0
10	7.0
11	7.0
12	7.0
13	25.5
14	44.0
15	62.0
16	80.0
17	64.5
18	49.0
19	39.0
20	29.0
21	49.5
22	70.0
23	78.0
24	86.0
25	187.5
26	289.0
27	392.0
28	495.0
29	457.5
30	420.0
31	381.0
32	342.0
33	410.0
34	478.0
35	568.5
36	659.0
37	848.5
38	1038.0
39	1089.0
40	1140.0
41	1092.5
42	1045.0
43	1067.5
44	1090.0
45	1067.0
46	1044.0
47	1279.5
48	1515.0
49	1565.0
50	1615.0
51	1753.5
52	1892.0
53	1921.0
54	1950.0
55	2158.0
56	2366.0
57	2073.5
58	1781.0
59	1764.5
60	1748.0
61	1511.5
62	1275.0
63	1039.5
64	804.0
65	753.0
66	702.0
67	620.0
68	538.0
69	451.0
70	364.0
71	341.5
72	319.0
73	275.0
74	231.0
75	169.5
76	108.0
77	96.5
78	85.0
79	75.5
80	66.0
81	57.0
82	48.0
83	33.0
84	18.0
85	12.0
86	6.0
87	9.0
88	12.0
89	9.0
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02318750966146236
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0038645849435770597
25	0.0
26	0.007729169887154119
27	0.0
28	0.007729169887154119
29	0.007729169887154119
30	0.007729169887154119
31	0.0
32	0.007729169887154119
33	0.007729169887154119
34	0.0038645849435770597
35	0.0
36	0.0
37	0.0038645849435770597
38	0.0
39	0.0
40	0.0
41	0.0038645849435770597
42	0.01159375483073118
43	0.015458339774308239
44	0.01159375483073118
45	0.0038645849435770597
46	0.0038645849435770597
47	0.0
48	0.01159375483073118
49	0.01159375483073118
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	25876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30267429278096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.81124497991968	27.198948832895347
2	14.895136099955378	12.899984541660226
3	7.246764837126284	9.414128922553717
4	4.078536367692994	7.064461276858866
5	2.829094154395359	6.12536713556964
6	1.9901829540383758	5.170814654506106
7	1.249442213297635	3.787293244705519
8	0.9817045961624274	3.400834750347813
9	0.6961178045515395	2.712938630391096
>10	3.1860776439089697	21.243623434843098
>50	0.035698348951361	0.9816045756685732
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	73	0.2821147008811254	No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC	69	0.2666563611068171	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	58	0.2241459267274695	No Hit
CTATAATACCTTGGAATACTGCCTGTTGCATGTGCTGGCCACATGCCTCC	54	0.20868758695316122	No Hit
CGGTAGAACATGGCGCAGGGACAACGCAAGTTCCAGGCGCAGAAACCTAA	50	0.19322924717885298	No Hit
CCTAAATAGAATCTAAGTGGCATTTAAAACAGTAAAGTTGTAGAGAATAG	49	0.18936466223527593	No Hit
GCTGAAGGGAGCATCAAAGAAATCAGAAGCCACCATCCCTGGCAAGACAC	47	0.18163549234812182	No Hit
CTCCAGGAAAGAGTGGTTGCACATGAAGATAAAAGGAAAAGGGTGGGGGT	43	0.16617715257381357	No Hit
ACACTGGAGCATCCCCAAGTGGCTCTGGATTACTGGTGTGACAGGCTGAC	42	0.1623125676302365	No Hit
CTCCAGAGGCATCGTGGGGATGCCAGATTGGGGAATCCTCCATGACCTGG	42	0.1623125676302365	No Hit
GTACATGGGTTGCGGTAGAACATGGCGCAGGGACAACGCAAGTTCCAGGC	41	0.15844798268665944	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	38	0.14685422785592828	No Hit
GCCCTACTGTGTGCAGAAAGAGCTCAGGGACTGCAGTGCTACGAGTGCTA	37	0.1429896429123512	No Hit
ATACTAGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGC	37	0.1429896429123512	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.13912505796877414	No Hit
TCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGG	36	0.13912505796877414	No Hit
GATTAAAAGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	35	0.1352604730251971	No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA	32	0.12366671819446591	No Hit
GTTTTAAAAGATTGAGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAAT	32	0.12366671819446591	No Hit
GTAGAACATGGCGCAGGGACAACGCAAGTTCCAGGCGCAGAAACCTAAAA	32	0.12366671819446591	No Hit
GTACATGGGACTGTGCCTGCAACCTTGTCTGAGAGGAACCCTTCTCTGAG	31	0.11980213325088886	No Hit
GCTCAAAACATAGAATTGATTGAGGACTCTCAAAGAAGGAAACTAAAGAC	30	0.1159375483073118	No Hit
GCCTAAGAGGCTTGCACTGCTGAAGGGAGCATCAAAGAAATCAGAAGCCA	30	0.1159375483073118	No Hit
CTATTAGCTTAAGTTTAGAGTTCTAATTCTTTTTACTGCTCAATCTTTTT	29	0.11207296336373475	No Hit
GTCCAGGTGCTACCTGCAGTGGGAACTGCTGCATTGCAGAGGTCTTCGCT	29	0.11207296336373475	No Hit
CCCCACAGGCTGGCTGGACTTGGAAGAGGGCCCCTGCTCCAGTCCTGGTG	28	0.10820837842015767	No Hit
CCCTTCAGCAGTGCAAGCCTCTTAGGCAGGCTGGAGCTGGCCTTCATTAC	28	0.10820837842015767	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	28	0.10820837842015767	No Hit
GTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGA	26	0.10047920853300356	No Hit
GTTTAATGGAGACAGGCTGGGTAAGTTGACGACGGTTCAGGTGGCTTTGA	26	0.10047920853300356	No Hit
GTACATGGGAGTGTGGGGCCTCACCGCGACGCCGCGGTCATGAGCCGGCC	26	0.10047920853300356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0038645849435770597	0.0
29	0.0	0.0	0.0	0.007729169887154119	0.0
30	0.0	0.0	0.0	0.007729169887154119	0.0
31	0.0	0.0	0.0	0.02318750966146236	0.0
32	0.0	0.0	0.0	0.030916679548616478	0.0
33	0.0	0.0	0.0	0.04637501932292472	0.0
34	0.0	0.0	0.0	0.05410418921007884	0.0
35	0.0	0.0	0.0	0.05410418921007884	0.0
36	0.0	0.0	0.0	0.08115628381511826	0.0
37	0.0	0.0	0.0	0.11207296336373473	0.0
38	0.0	0.0	0.0	0.14685422785592828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	45	9.860887E-7	34.22222	1
ATGGGGA	35	3.1322378E-4	31.428572	5
CTATAAT	30	0.0056429496	29.333332	1
GTACTTT	30	0.0056429496	29.333332	14
TTATTTA	30	0.0056429496	29.333332	25
AGCAGCA	30	0.0056429496	29.333332	44
GCTGCAT	30	0.0056429496	29.333332	39
TGGGGAG	40	6.8464805E-4	27.5	6
CATGGGG	60	9.28048E-6	25.666666	4
AGTACTT	45	0.001361591	24.444445	13
GTACATG	140	7.2759576E-12	22.0	1
TATCAAC	75	5.212091E-5	20.533333	2
TACATGG	155	2.7284841E-11	19.870968	2
AGTACAT	70	7.8335626E-4	18.857143	12
GAGTACA	70	7.8335626E-4	18.857143	11
ACATGGG	165	6.91216E-11	18.666666	3
TCAACGC	85	1.3635066E-4	18.117647	4
ATCAACG	90	2.1117424E-4	17.11111	3
ACGCAGA	95	3.190703E-4	16.210526	7
CAGAGTA	95	3.190703E-4	16.210526	10
>>END_MODULE