FastQCFastQC Report
Thu 2 Feb 2017
SRR4061980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004770
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75870.7550981816734178No Hit
TATCAACGCAGAGTACTTTTTTTTT48120.4789155727181345No Hit
GGTATCAACGCAGAGTACTTTTTTT29580.2943957323566587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24160.24045303900395112No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15410.15336843257660956No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13140.13077619753774494No Hit
GTCCTACAGTGGACATTTCTAAATT12050.11992794370851041No Hit
CCATAGGGTCTTCTCGTCTTATTAT11450.11395642783920698No Hit
GGTATCAACGCAGAGTACATGGGGG11000.10947779093722941No Hit
GTATCAACGCAGAGTACATGGGAGA10340.10290912348099564No Hit
GTATCAACGCAGAGTACATGGGGGA10050.10002289081083232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA250.006025141619.002997
CGGGTTC401.285486E-518.99825717
CGACGGG504.5630404E-617.0984314
GACGGGT504.5630404E-617.0984315
GGCGATC350.002167199716.2882778
CATACGC508.7047505E-515.2008773
GTCCTAT851.720764E-814.5251841
CGTCTTA1450.014.4124715
GGTATCA15900.014.2757191
CGGTCCA400.005273616414.2515339
TCACCGA400.005275360314.2508223
CAGCCGA602.5650768E-514.25082110
GCGCCAC801.2882992E-714.24869213
AACGGAC954.8985385E-913.99871515
TGCGCCA1101.8553692E-1013.81897812
CGTGCCG707.2483563E-613.57288811
TTCTCGT1400.013.57288811
CCGACGG707.2632956E-613.57018313
TGATCGA500.001498249813.30076810
TCTCGTC1450.013.10420412
Produced by FastQC (version 0.11.5)