##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061980_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004770
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11890880500015	32.0	32.0	32.0	32.0	32.0
2	30.84891965325398	32.0	32.0	32.0	32.0	32.0
3	30.895636812404828	32.0	32.0	32.0	32.0	32.0
4	30.870997342675437	32.0	32.0	32.0	32.0	32.0
5	30.88274630014829	32.0	32.0	32.0	32.0	32.0
6	34.47499825830787	36.0	36.0	36.0	32.0	36.0
7	34.3883963494133	36.0	36.0	36.0	32.0	36.0
8	34.340645122764414	36.0	36.0	36.0	32.0	36.0
9	34.380715984752726	36.0	36.0	36.0	32.0	36.0
10	34.22028822516596	36.0	36.0	36.0	32.0	36.0
11	34.45273943290504	36.0	36.0	36.0	32.0	36.0
12	34.33136538710352	36.0	36.0	36.0	32.0	36.0
13	34.37234292425132	36.0	36.0	36.0	32.0	36.0
14	34.31193706022274	36.0	36.0	36.0	32.0	36.0
15	34.285244384286955	36.0	36.0	36.0	32.0	36.0
16	34.27955153915821	36.0	36.0	36.0	32.0	36.0
17	34.21376533933139	36.0	36.0	36.0	32.0	36.0
18	34.22235337440409	36.0	36.0	36.0	32.0	36.0
19	34.20329627675986	36.0	36.0	36.0	32.0	36.0
20	34.14086805935687	36.0	36.0	36.0	32.0	36.0
21	34.13266319655244	36.0	36.0	36.0	32.0	36.0
22	34.097149596425055	36.0	36.0	36.0	32.0	36.0
23	34.09961284672114	36.0	36.0	36.0	32.0	36.0
24	34.08015167650308	36.0	36.0	36.0	32.0	36.0
25	33.76528260198852	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	10.0
5	73.0
6	215.0
7	35.0
8	167.0
9	128.0
10	61.0
11	22.0
12	40.0
13	46.0
14	243.0
15	338.0
16	579.0
17	723.0
18	971.0
19	1263.0
20	1838.0
21	2423.0
22	3268.0
23	4300.0
24	5782.0
25	7533.0
26	10211.0
27	12986.0
28	17583.0
29	23715.0
30	31788.0
31	43900.0
32	63654.0
33	91733.0
34	207818.0
35	471324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.889590878645016	19.500519027870254	13.311696390093983	22.29819370339075
2	14.691252109939807	21.35935857829867	41.525904487404055	22.423484824357466
3	18.36105449218128	27.87907258192936	29.134188190706713	24.62568473518265
4	10.903118888009674	17.16627599695427	37.94253920383006	33.988065911206
5	12.211930182269992	40.18133415103281	32.72317819609655	14.88355747060065
6	29.59651526860475	38.24640094328349	19.08017909639178	13.076904691719978
7	27.994544278915274	34.01197747970793	19.7947230145549	18.198755226821902
8	28.018766788085543	33.49566797650266	18.862435338471716	19.623129896940085
9	28.554773909404336	15.154746009286754	20.08110962753343	36.209370453775485
10	16.108017284678983	29.15284742762838	30.73252597086798	24.006609316824658
11	33.948876802602506	24.536523475069792	22.279763310778687	19.234836411549015
12	24.668512994888548	27.52739844115153	28.897195926384722	18.906892637575204
13	28.4101644245142	23.111808669656202	26.16462381664175	22.31340308918784
14	22.984497321231963	22.09781862493561	27.979251379737413	26.938432674095015
15	24.323392415457683	29.345505858717118	24.882955209066214	21.44814651675898
16	22.763931956412566	29.170918062320144	25.943610550901163	22.121539430366123
17	20.449107912450362	28.738422292971418	26.810157989925713	24.002311804652507
18	20.52821511195777	28.27349698410453	29.535151571912223	21.66313633202548
19	23.585866132711715	26.224061843140774	29.471225207455447	20.718846816692068
20	25.900904490576565	25.520580149021793	28.325397457863488	20.25311790253815
21	26.649792207067748	24.935749548752646	26.986982559737978	21.427475684441628
22	24.494474260204413	27.103571880445166	27.417691732162265	20.984262127188156
23	23.090185782073604	27.394688298080027	27.908447645950286	21.606678273896087
24	22.49442918717433	26.99630339089923	28.520071362187004	21.989196059739434
25	22.328330198903483	27.29294832972077	28.783529102384293	21.595192368991455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	295.0
1	295.0
2	562.5
3	830.0
4	830.0
5	830.0
6	1882.0
7	2934.0
8	2934.0
9	2934.0
10	3078.0
11	3222.0
12	3222.0
13	3222.0
14	3831.5
15	4441.0
16	4441.0
17	4441.0
18	7365.0
19	10289.0
20	10289.0
21	10289.0
22	17052.5
23	23816.0
24	23816.0
25	23816.0
26	35729.5
27	47643.0
28	47643.0
29	47643.0
30	60384.0
31	73125.0
32	73125.0
33	73125.0
34	81215.5
35	89306.0
36	89306.0
37	89306.0
38	97036.5
39	104767.0
40	104767.0
41	104767.0
42	110310.0
43	115853.0
44	115853.0
45	115853.0
46	127882.0
47	139911.0
48	139911.0
49	139911.0
50	139621.0
51	139331.0
52	139331.0
53	139331.0
54	120805.0
55	102279.0
56	102279.0
57	102279.0
58	82838.0
59	63397.0
60	63397.0
61	63397.0
62	50274.0
63	37151.0
64	37151.0
65	37151.0
66	29827.5
67	22504.0
68	22504.0
69	22504.0
70	17301.0
71	12098.0
72	12098.0
73	12098.0
74	9505.5
75	6913.0
76	6913.0
77	6913.0
78	4729.0
79	2545.0
80	2545.0
81	2545.0
82	1842.0
83	1139.0
84	1139.0
85	1139.0
86	802.5
87	466.0
88	466.0
89	466.0
90	292.0
91	118.0
92	118.0
93	118.0
94	90.0
95	62.0
96	62.0
97	62.0
98	198.5
99	335.0
100	335.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.966768514187326E-4
2	1.9905052897678077E-4
3	1.9905052897678077E-4
4	0.008459647481513182
5	0.03244523622321526
6	0.06150661345382526
7	0.10480010350627507
8	0.10768633617643839
9	0.11843506474118455
10	0.13316480388546634
11	0.14222160295390984
12	0.13306527862097794
13	0.12639708590025578
14	0.11316022572329985
15	0.13017904595081461
16	0.11724076156732387
17	0.12191844899827821
18	0.10828348776336873
19	0.09355374861908695
20	0.08857748539466744
21	0.08817938433671388
22	0.10081909292673945
23	0.07942116106173552
24	0.08728365695631836
25	0.08618887904694607
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1004770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.76575752812117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.31020615110402	23.276476884068565
2	15.24629850277318	11.210834279086315
3	7.199594666419179	7.940956554189659
4	3.997821904521691	5.879318031290244
5	2.477532954300957	4.554418793287935
6	1.69979610494673	3.7496574865029784
7	1.2081239217455921	3.10923138195749
8	0.8575679116344661	2.5223307122440017
9	0.627873475919807	2.0775819576605628
>10	3.102339693240527	19.34373373218197
>50	0.16094510635863774	4.130507352616001
>100	0.09753960905843714	6.839722432580335
>500	0.011379621038237466	2.763601690530108
>1k	0.00270943358053273	1.8458544254594997
>5k	2.7094335805327306E-4	0.7557742863443727
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7587	0.7550981816734178	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4812	0.4789155727181345	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2958	0.2943957323566587	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2416	0.24045303900395112	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1541	0.15336843257660956	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1314	0.13077619753774494	No Hit
GTCCTACAGTGGACATTTCTAAATT	1205	0.11992794370851041	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1145	0.11395642783920698	No Hit
GGTATCAACGCAGAGTACATGGGGG	1100	0.10947779093722941	No Hit
GTATCAACGCAGAGTACATGGGAGA	1034	0.10290912348099564	No Hit
GTATCAACGCAGAGTACATGGGGGA	1005	0.10002289081083232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	25	0.0060251416	19.00299	7
CGGGTTC	40	1.285486E-5	18.998257	17
CGACGGG	50	4.5630404E-6	17.09843	14
GACGGGT	50	4.5630404E-6	17.09843	15
GGCGATC	35	0.0021671997	16.288277	8
CATACGC	50	8.7047505E-5	15.200877	3
GTCCTAT	85	1.720764E-8	14.525184	1
CGTCTTA	145	0.0	14.41247	15
GGTATCA	1590	0.0	14.275719	1
CGGTCCA	40	0.0052736164	14.251533	9
TCACCGA	40	0.0052753603	14.250822	3
CAGCCGA	60	2.5650768E-5	14.250821	10
GCGCCAC	80	1.2882992E-7	14.248692	13
AACGGAC	95	4.8985385E-9	13.998715	15
TGCGCCA	110	1.8553692E-10	13.818978	12
CGTGCCG	70	7.2483563E-6	13.572888	11
TTCTCGT	140	0.0	13.572888	11
CCGACGG	70	7.2632956E-6	13.570183	13
TGATCGA	50	0.0014982498	13.300768	10
TCTCGTC	145	0.0	13.104204	12
>>END_MODULE