FastQCFastQC Report
Thu 2 Feb 2017
SRR4061980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004770
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.17277585915184568No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG12600.12540183325537188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.10798491196990355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTCG300.005744970429.33290310
GTATCAA53250.026.3222221
GGTATCA19650.026.3152851
TATACCG450.001398169724.4440845
GTATTAG2700.022.0040591
GTACCGT703.2150227E-521.9996766
TACCGTG956.8649024E-720.8417997
CTATTAC1301.6189006E-920.3114361
TTTCGCG550.004483476319.99970425
CGATATT550.004483476319.99970427
GATATAC1900.019.687841
GGTCGCG808.987661E-519.2497187
GCGGTAT2650.019.09595944
TATCAAC76400.018.9771062
CGTCTTA3200.018.56222715
ATACTGC1557.203198E-1018.4513426
TACGCCG600.007411881318.33306516
CTATACT1801.4551915E-1118.3330634
CGCTCTA851.430689E-418.1173815
TTCGACT851.430689E-418.1173822
Produced by FastQC (version 0.11.5)